GLUT4-IN-2 , CAS No.2454113-83-6

CAS: 2454113-83-6 Cat. No.: G648259 Formula: C₁₇H₁₁N₃O₄S₂ Peso molecolare: 385.42

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

GLUT4-IN-2 is a potent and selective GLUT4 inhibitor with IC 50 s of 11.4 µM and 6.8 µM for GLUT1 and GLUT4 , respectively. GLUT4-IN-2 induces cell apoptosis and cell cycle arrest at G0/G1phase. GLUT4-IN-2 shows potent antitumor activity

In Vitro

GLUT4-IN-2 (compound F18) induces cell apoptosis and ell cycle arrest at G0/G1phase in CME cells. GLUT4-IN-2 (10 µM; 6 h) decreases the expression of mTOR and CDK2, but increases the expression of GRP78, and cleaved caspase 3 proteins. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: CME, K562, KCL-22, MB-231, HS-27 cells Concentration: 1-100 µM Incubation Time: 48 h Result: Showed potent cytotoxicity with cytotoxic concentration 50% (CC 50 ) of 1.7, 91.9, 15.3, 45.1, 44.0 µM for CME, K562, KCL-22, MB-231, HS-27 cells, respectively. Apoptosis AnalysisCell Line: CEM cells Concentration: 1.7 µM Incubation Time: 24 h Result: Induced cell apoptosis with the percentage of apoptotic cells in the late and early apoptosis region was 55.87% and 1.38%, respectively. Cell Cycle AnalysisCell Line: CEM cells Concentration: 10, 25, 50 µM Incubation Time: 72 h Result: Induced cell cycle arrest at G0/G1phase in a dose-dependent manner. Western Blot AnalysisCell Line: CEM cells Concentration: 10 µM Incubation Time: 6 h Result: Decreased the phosphorylation of mTOR and CDK2 proteins and increased the expression of GRP78, and cleaved caspase 3. Cell Cytotoxicity AssayCell Line: CEM cells Concentration: 2.5-100 µM Incubation Time: 48 h Result: Showed cytotoxicity with the IC 50 s of 1.7, 187.2 µM for CEM, WBCs cells respectively.

In Vivo

GLUT4-IN-2 (50 mg/kg; i.p. on day 1–5, 8–12, 15–18) shows antitumor activity in CEM xenograft model . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: 8–10 weeks, SCID mice (CEM xenograft tumor) Dosage: 50 mg/kg Administration: I.p.; administered on day 1-5, 8-12, 15-18 Result: Showed potent antitumor activity in vivo.

Form:Solid

IC50& Target:GLUT1 11.4 μM (IC 50 ) GLUT4 6.8 μM (IC 50 )

Specifications

Meccanismi biochimici e fisiologici

GLUT4-IN-2 is a potent and selective GLUT4 inhibitor with IC 50 s of 11.4 µM and 6.8 µM for GLUT1 and GLUT4 , respectively. GLUT4-IN-2 induces cell apoptosis and cell cycle arrest at G0/G1phase. GLUT4-IN-2 shows potent antitumor activity.

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Tipo di azione

INHIBITOR

Nomi e identificatori

• Peso molecolare: 385.42

Domande frequenti

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2454113-83-6, the molecular formula is C17H11N3O4S2, and the molecular weight is 385.42 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.