HA tag Mouse mAb - Anticorpo primario, specific to HA, Mouse IgG1

Cat. No.: Ab106776

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Indipendente dalla specie

Isotype

Mouse IgG1

Clone Number

4G3

Conjugation

Unconjugated

Panoramica

Mouse anti HA tag Antibody, Monoclonal (4G3), could be used for WB, IF, ICC and so on.
Application:
WB: 1/1000 - 1/2000
IF/ICC: 1/1000
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Specifications

Product Name

HA tag Mouse mAb - Anticorpo primario, specific to HA, Mouse IgG1

Sinonimi

HA epitope tag antibody | HA1 antibody | HA2 antibody | hemagglutinin antibody | Hemagglutinin HA1 chain antibody | Hemagglutinin HA2 chain antibody | HA Epitope Tag | HA Tag | HA | Hemagglutinin | HA tag antibody | Anti- HA tag antibody

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Specie ospite

Mouse

Specificità

HA

Immunogeno

Synthetic peptide HA-tag.

Controllo positivo

WB: Over-expressed HA-tagged protein in HEK293T cell lysate. IF/ICC: HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62 #PPL00549-2b.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

1.0 mg/mL

Nota

This product is being discontinued. Replacement product is Ab106781.

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: HA tag Mouse mAb (Ab106776)

HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 75 kDa
Observed band size: 77 kDa
Exposure time: 30.0 s

Western Blot: HA tag Mouse mAb (Ab106776)

HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 30.0 s

IF/ICC: HA tag Mouse mAb (Ab106776)

HA tag Mouse mAb (Ab106776) - IF/ICC
IF image of HA tag Mouse mAb (Ab106776) stained HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62. The cells were 4% para-formaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1 h to permeabilise the cells and block non-specific pro-tein-protein interactions. The cells were then incubated with the HA tag Mouse mAb (Ab106776) (1/200) and the antibody Flag-tag pAb(1/200) at 5 µg/mL overnight at 4°C. The secondary anti-body (Red) was Goat anti-Mouse IgG (H+L)-TRITC and the secondary antibody (Green) was Goat anti-Rabbit IgG (H+L)-FITC used at 1/1000 dilution for 1 h. Hoechst33342 was used to stain the cell nuclei (blue).

Applicazione

• Applicazione: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/1000;

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HA tag Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.