Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated per flussi di lavoro cromatografici e analitici sensibili che richiedono un'interferenza minima della linea di base.

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Stoccaggio e spedizione

Store at -20°C,Avoid repeated freezing and thawing Spedizione Ice chest + Ice pads. Consultare il COA specifico del lotto per le specifiche esatte.

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Documenti di qualità

SDS, COA, scheda tecnica e scheda delle specifiche disponibili per il download. COA specifico del lotto accessibile tramite ricerca per numero di lotto.

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Prova documentale

Citato in 0 pubblicazioni peer-reviewed su cromatografia, sintesi organica e reazioni di accoppiamento incrociato.

Panoramica

Mouse anti Human HDAC1 Antibody, Monoclonal (E22), could be used for WB, IHC, IF, ICC and so on.

Application:

WB: 0.2-2μg/mL

IHC: 5-20μg/mL

ICC/IF: 5-20μg/mL

Protein Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Post-translational

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.

Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.

Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Specifiche

Product Name

HDAC1 Mouse mAb - Anticorpo primario, specific to HDAC1, Mouse IgG1

Sinonimi

Specifiche e purezza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Specie ospite

Mouse

Specificità

HDAC1

Immunogeno

Recombinant HDAC1 expressed in E.coli (AA 1-482) .

Controllo positivo

WB: A549, HeLa, HepG2, Jurkat, THP-1, HEK293 and NIH/3T3 cell lysates; IHC: Human Placenta Tissue, Human Lung cancer Tissue, Rat Colon Tissue, Rat Testis Tissue, Mouse Pancreas Tissue, Mouse Cerebellum Tissue; IF: HeLa.

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A+G Affinity Chromatography

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: HDAC1 Mouse mAb (Ab107009)

HDAC1 Mouse mAb (Ab107009) - Western Blot
All lanes: HDAC1 Mouse mAb (Ab107009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 63 kDa
Exposure time: 1.2 s