Heinz Body Staining Solution (Nile Blue Method) - BioReagent,for microscopy,Biological Stain

Cat. No.: H1510337

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Synonyms

Heinz Body Stain Solution (Nile Blue Method)

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining, Hematologic staining

Panoramica

  Denatured globin bodies, also known as hemoglobin inclusions, are essentially denatured hemoglobin particles that typically adhere to the cell membrane. They often occur in susceptible individuals after taking certain medications or exposure to chemicals, which can induce hemoglobin denaturation. Such inclusions may also be observed in patients with unstable hemoglobin disorders. Therefore, they hold diagnostic significance for conditions such as glucose-6-phosphate dehydrogenase deficiency and unstable hemoglobinopathies. After staining, these inclusions appear as small blue-black dots under the microscope. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other applications.

Procedure (for reference only)

1. Add one drop of Heinz body staining solution (methylene blue method) onto one end of a glass slide and allow it to air-dry.

2. Place one drop of the test blood sample onto the dried stain film and mix gently using the corner of another glass slide.

3. Cover with a coverslip and let it stand for 5–10 minutes before observing under oil immersion.

4. Counting: Examine 500–1,000 red blood cells, count the number of Heinz body-positive red blood cells, and calculate the Heinz body positivity rate.

Staining Results

Heinz bodies	Blue-black dots
Red blood cells	Yellow-green

Precautions

1. Normally, Heinz bodies are absent or only a few tiny Heinz bodies may occasionally be observed.

2. In general, Heinz bodies are located on the red blood cell membrane or scattered within the red blood cells.

3. For your safety and health, please wear a lab coat and disposable gloves during operation.

4. After opening, the reagent should be used as soon as possible to avoid affecting subsequent experimental results.

Specifications

Sinonimi

Heinz Body Stain Solution (Nile Blue Method)

Specifiche e purezza

BioReagent,for microscopy,Biological Stain

Stabilità e conservazione

Store at room temperature long term (12 months). Store in the dark.

Condizioni di conservazione di stoccaggio

Protected from light,Room temperature

Spedito in

Normal

Grado

Biological Stain, BioReagent, per la microscopia

Product Line

Prodotti biologici

Proprietà chimiche e fisiche

• Sensibilità: Light-sensitive

Domande frequenti

What does Biological Stain mean?

Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.