HERPUD1 Mouse mAb - Anticorpo primario, specific to HERPUD1

Cat. No.: Ab107327

GRADE & PURITY Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Mouse

Reactive species

consiglio, Mouse, Umano

Isotype

Mouse IgG2a

Clone Number

OTI1A2

Conjugation

Unconjugated

Panoramica

Mouse anti Human HERPUD1 Antibody, Monoclonal (OTI1A2), could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

HERPUD1 Mouse mAb - Anticorpo primario, specific to HERPUD1

Sinonimi

SUP | HERP | Mif1 | HERPUD1 | HERP antibody | HERP1_HUMAN antibody | HERPUD1 antibody | Homocysteine inducible endoplasmic reticulum stress inducible ubiquitin like domain member 1 antibody | Homocysteine inducible endoplasmic reticulum stress inducible u

Specifiche e purezza

ExactAb™, Validated, High Performance, See COA

Meccanismi biochimici e fisiologici

Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated beta-amyloid prote

Specie ospite

Mouse

Specificità

HERPUD1

Immunogeno

Recombinant full length protein of human HERPUD1 produced in HEK293T.

Controllo positivo

WB: Hep G2, HeLa, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human kidney, pancreas, pancreas carcinoma, endometrium and prostate tissues. IF/ICC: COS7 cells transfected with the pCMV6-ENTRY HERPUD1.

Coniugazione

Unconjugated

Grado

ExactAb™, High Performance, Validated

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - Western Blot
All lanes: HERPUD1 Mouse mAb (Ab107327) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 51 kDa
Exposure time: 80.0 s

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IF/ICC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IF/ICC
HERPUD1 Mouse mAb (Ab107327) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HERPUD1.

Applicazione

• Applicazione: Dilution info
• IF/ICC:

  1/100;

• IHC:

  1/150

• WB:

  1/400-1/4000

Obiettivi associati (umani)

HERPUD1 Tbio Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (0 Activities)

Discover Target: HERPUD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HERPUD1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.