hHR23b Mouse mAb - Anticorpo primario, specific to hHR23b, Mouse IgG2b

Cat. No.: Ab107446

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Mouse

Reactive species

consiglio, Criceto, Mouse, Scimmia, Umano

Isotype

Mouse IgG2b

Clone Number

5H1-A10-A7

Conjugation

Unconjugated

Panoramica

Mouse anti Human hHR23b Antibody, Monoclonal (5H1-A10-A7), could be used for WB, IHC, ICC, IF and so on.

Application:

WB: 1/500-1/1000 

IHC: 1/50-1/100

ICC/IF: 1/50-1/200

Protein Function:

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmatic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

Specifications

Product Name

hHR23b Mouse mAb - Anticorpo primario, specific to hHR23b, Mouse IgG2b

Sinonimi

hHR23B | HR23B | p58 | XP-C repair-complementing complex 58 kDa protein | hHR 23b antibody | hHR23B antibody | HR 23B antibody | HR23B antibody | mHR 23B antibody | mHR23B antibody | p58 antibody | RAD 23B antibody | RAD23 (S. cerevisiae) homolog B antibo

Specifiche e purezza

ExactAb™, Validated, 1.0 mg/mL

Specie ospite

Mouse

Specificità

hHR23b

Immunogeno

Recombinant protein of human hHR23b (AA 1-409)

Controllo positivo

WB: HeLa, K562, MCF7, NIH/3T3 and C6 cell lysates. IHC: Prostate cancer tissue. ICC/IF: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Mouse IgG2b

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

1.0 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: hHR23b Mouse mAb (Ab107446)

hHR23b Mouse mAb (Ab107446) - Western Blot
All lanes: hHR23b Mouse mAb (Ab107446) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 54 kDa
Exposure time: 5.2 s

IF/ICC: hHR23b Mouse mAb (Ab107446)

hHR23b Mouse mAb (Ab107446) - IF/ICC
Immunocytochemistry analysis of Rad23B (5H1) in HeLa using hHR23b Mouse mAb (Ab107446).

IHC: hHR23b Mouse mAb (Ab107446)

hHR23b Mouse mAb (Ab107446) - IHC
Immunohistochemistry analysis of paraffin-embedded Prostate Cancer using hHR23b Mouse mAb (Ab107446). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

Obiettivi associati (umani)

RAD23B Tbio UV excision repair protein RAD23 homolog B (0 Activities)

Discover Target: RAD23B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (hHR23b Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.