His tag (C-terminal) Mouse mAb - Anticorpo primario, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
His tag (C-terminal) Mouse mAb - Anticorpo primario, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Mouse anti His tag (C-terminal) Antibody, clone IJ08/4E6, could be used for WB, IF/ICC, ELISA and so on.
Specifications
Product Name
His tag (C-terminal) Mouse mAb - Anticorpo primario, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
Sinonimi
6 His epitope tag antibody | Hexa His tag antibody | HHHHHH epitope tag antibody | HHHHHH tag antibody | His tag antibody | Polyhistidine Tag antibody | His tag Mouse Monoclonal Antibody | His tag Mouse mAb | Anti-His tag | Monoclonal Anti- His tag antibo
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized met
Specie ospite
Mouse
Specificità
Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
Immunogeno
A recombinant protein containing the sequence HHHHHH
Controllo positivo
WB: His-transfected HEK293 whole cell lysates; C-terminal His fusion proteins IF/ICC: HeLa cells transfected with eGFP-His
Coniugazione
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo di catena leggera
kappa
SDS-PAGE
150.0 KDa
Metodo di purificazione
Protein A purified
Concentrazione
1.0 mg/mL
Nota
This product can replace Ab086831, Ab155835, Ab219141 and Ab219145.
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
His tag (C-terminal) Mouse mAb (Ab219147) - Western Blot All lanes: His tag (C-terminal) Mouse Antibody (Ab219147) at 1/20000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 48,116 kDa Observed band size: 50,115 kDa Exposure time: 107.5 s
His tag (C-terminal) Mouse mAb (Ab219147) - Western Blot All lanes: His tag (C-terminal) Mouse Antibody (Ab219147) at 1/20000 dilution Samples: Lysates at 10 ng per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 35,70 kDa Observed band size: 35,67 kDa Exposure time: 100.0 s
His tag (C-terminal) Mouse mAb (Ab219147) - Western Blot All lanes: His tag (C-terminal) Mouse Antibody (Ab219147) at 1/20000 dilution Samples: His-tagged protein at various amounts Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 120.0 s
His tag (C-terminal) Mouse mAb (Ab219147) - ICC/IF IF analysis of His tag (C-terminal) (Green) in HeLa cells transfected with eGFP-His. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with His tag (C-terminal) Mouse mAb (Ab219147) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Applicazione
Applicazione
Dilution info
IF/ICC
1:500
WB
1/1000 - 1/200000
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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