Hoechst 33342 Staining Solution - Suitable for Immunofluorescence(IF), BioReagent, ready-to-use, Biological Stain, for fluorescence analysis, Biological dye grade, for microscopy, for cell culture, 1.0 mg/ml in H₂O , CAS No.23491-52-3

CAS: 23491-52-3 Cat. No.: O1501802 Molecular Weight: 561.93（anhydrous basis） Beilstein Registry Number: 1234011 EC Number: 245-691-1

GRADE & PURITY BioReagent, Ready-to-use, Biological Stain, for Fluorescence analysis, Biological dye grade, for Microscopy, for Cell culture, Suitable for Immunofluorescence(IF), 1.0 mg/ml in H₂O

Synonyms

Hoechst 33342 Stain Solution (1 mg/mL) | Hoechst 33342 Staining Solution for Live Cells, 100X | Hoechst 33342 Solution

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Cell Apoptosis Analysis, Cell Cycle Analysis, Cell Stain, Flow Cytometry, IF/ICC

Overview

Hoechst 33342 solution is a reagent used for fluorescent staining of DNA and cell nuclei in living cells or fixed cells. Hoechst 33342 is a bis-benzimidazole dye with high specificity for double-stranded DNA (it is more inclined to bind to A-T base pairs). This dye is very helpful for marking double-stranded DNA, thereby enabling visualization of the cell nucleus. Hoechst 33342 can be excited by a UV light source at approximately 355 nm. When combined with DNA, it emits blue fluorescence at the emission peak of 461 nm.

Hoechst 33342 is commonly used for cell apoptosis detection. After staining, it can be observed using a fluorescence microscope or detected by a flow cytometer. It can also be used for ordinary cell nucleus staining or routine DNA staining.

The concentration of this product is 1.0 mg/mL. When used for cell nucleus staining, the recommended working concentration is 1-10 μg/mL.

Instruction for use 

1.For fixed cells or tissues:

a. The cells were inoculated onto the culture plates/flasks and cultured until they reached the logarithmic growth phase. If cell fixation was required, the cells were fixed with 4% paraformaldehyde at room temperature for 10-15 minutes, then washed twice with PBS, each time for 5 minutes.

b. Add the Hoechst 33342 working solution and incubate at 37°C in the dark for 10-30 minutes.

c. Remove the Hoechst 33342 staining solution and wash the cells twice with PBS for 5 minutes each time.

2.For living cells or tissues：

a. Collect the cells, centrifuge at 1000 rpm for 5 minutes, and discard the supernatant.

b. Resuspend the cells in PBS, add the Hoechst 33342 working solution, and incubate at room temperature in the dark for 15-20 minutes.

c. Centrifuge at 1000 rpm for 5 minutes, discard the staining solution, wash twice with PBS for 5 minutes each time; then resuspend the cells in PBS.

d. Observe directly under a fluorescence microscope or under a fluorescence microscope after mounting. When cells undergo apoptosis, the nuclei of apoptotic cells will show dense and compact staining, or fragmented and dense staining.

Matters needing attention

1.Depending on the application of Hoechst 33342 staining solution, it is recommended to perform a titration before use to determine the optimal working concentration.

2.Fluorescent dyes all have the problem of quenching. It is recommended to complete the detection as soon as possible after staining. Live cells or tissues should be observed immediately after staining.

3.This product is only for professional scientific research use. It shall not be used for clinical diagnosis or treatment, nor for food or medicine. It shall not be stored in ordinary residences.

4.For your safety and health, please wear laboratory coats and disposable gloves during operation.

Specifications

Synonyms

Hoechst 33342 Stain Solution (1 mg/mL) | Hoechst 33342 Staining Solution for Live Cells, 100X | Hoechst 33342 Solution

Specifications & Purity

Suitable for Immunofluorescence(IF), BioReagent, ready-to-use, Biological Stain, for fluorescence analysis, Biological dye grade, for microscopy, for cell culture, 1.0 mg/ml in H₂O

Stability And Storage

Store at -20℃ long term (12 months). Store in the dark. Avoid repeated freezing and thawing.

Storage

Protected from light, Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Biological dye grade, Biological Stain, BioReagent, for Cell culture, for Fluorescence analysis, for Microscopy, Ready-to-use, Suitable for Immunofluorescence(IF)

Images

Cell Staining: Hoechst 33342 Staining Solution (O1501802)

Hoechst 33342 Staining Solution (O1501802) - Cell Staining 

Apoptosis was induced in Jurkat cells by a 16-hour treatment with 2 μM camptothecin (C111281) and assessed using Hoechst 33342 staining solution (O1501802). This dye exhibits enhanced binding to condensed and fragmented chromatin in apoptotic cells, resulting in intense blue fluorescence under confocal laser scanning microscopy. In contrast, viable cells show only weak, diffuse nuclear fluorescence.

Names and Identifiers

• Isomeric SMILES: CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C
• WGK Germany: 3
• Alternate CAS: 875756-97-1
• Molecular Weight: 561.93（anhydrous basis）
• Beilstein: 1234011
• Reaxy-Rn: 9095542
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=9095542&ln=

Application

• Application: Dilution info
• Flow Cytometry:

  1-10 μg/ml ;

Chemical and Physical Properties

• Sensitivity: Light-sensitive

References

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51. Fabiao Hu, Ruihuan Zhang, Wei Guo, Ting Yan, Xiujuan He, Fengzhi Hu, Fuzheng Ren, Xingyuan Ma, Jiandu Lei, Wenyun Zheng.  (2020)  PEGylated-PLGA Nanoparticles Coated with pH-Responsive Tannic Acid–Fe(III) Complexes for Reduced Premature Doxorubicin Release and Enhanced Targeting in Breast Cancer.  MOLECULAR PHARMACEUTICS,      [PMID:32515968] [10.1021/acs.molpharmaceut.0c00321]

52. Shuai Guo, Liang Qiu, Yafei Chen, Xuzhao Wang, Biao Ma, Chang Qu, Jianmin Cui, Hailin Zhang, Chengfen Xing, Yong Zhan, Hailong An.  (2020)  TMEM16A-inhibitor loaded pH-responsive nanoparticles: A novel dual-targeting antitumor therapy for lung adenocarcinoma.  BIOCHEMICAL PHARMACOLOGY,      [PMID:32492446] [10.1016/j.bcp.2020.114062]

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56. Jizheng Song, Yuling Liu, Longfei Lin, Ye Zhao, Xiuqing Wang, Ming Zhong, Tanggui Xie, Yuting Luo, Shaojing Li, Ruocong Yang, Hui Li.  (2019)  Glycyrrhetinic acid modified and pH-sensitive mixed micelles improve the anticancer effect of curcumin in hepatoma carcinoma cells.  RSC Advances,  9  (68): (40131-40145).  [PMID:35541419] [10.1039/C9RA07250K]

57. Fei Gao, Jing Zhao, Ping Liu, Dongsheng Ji, Litao Zhang, Mengxiao Zhang, Yuqin Li, Yuliang Xiao.  (2019)  Preparation and in vitro evaluation of multi-target-directed selenium-chondroitin sulfate nanoparticles in protecting against the Alzheimer's disease.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:31593732] [10.1016/j.ijbiomac.2019.09.098]

58. Asif Jan, Chengcheng Liu, Hong Deng, Jiao Li, Wenpeng Ma, Xiaoyan Zeng, Yanhong Ji.  (2019)  In vitro photodynamic inactivation effects of hypocrellin B on azole-sensitive and resistant Candida albicans.  Photodiagnosis and Photodynamic Therapy,      [PMID:31325521] [10.1016/j.pdpdt.2019.07.014]

59. Xu-Feng Wang, Jin Ren, Hai-Qing He, Liang Liang, Xin Xie, Zi-Xin Li, Jian-Guo Zhao, Jing-Mou Yu.  (2019)  Self-assembled nanoparticles of reduction-sensitive poly (lactic-co-glycolic acid)-conjugated chondroitin sulfate A for doxorubicin delivery: preparation, characterization and evaluation.  PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY,      [PMID:30907676] [10.1080/10837450.2019.1599914]

60. Feng Bai, Ying Wang, Qianqian Han, Mingliang Wu, Qiang Luo, Hongmei Zhang, Yanqing Wang.  (2019)  Cross-linking of hyaluronic acid by curcumin analogue to construct nanomicelles for delivering anticancer drug.  JOURNAL OF MOLECULAR LIQUIDS,      [PMID:] [10.1016/j.molliq.2019.111079]

61. Yukun Song, Yanyang Wu, Haitao Wang, Shan Liu, Liang Song, Shen Li, Mingqian Tan.  (2019)  Carbon quantum dots from roasted Atlantic salmon (Salmo salar L.): Formation, biodistribution and cytotoxicity.  FOOD CHEMISTRY,      [PMID:31151626] [10.1016/j.foodchem.2019.05.017]

62. Ping Liu, Nana Chen, Lei Yan, Fei Gao, Dongsheng Ji, Shijiao Zhang, Litao Zhang, Yuqin Li, Yuliang Xiao.  (2019)  Preparation, characterisation and in vitro and in vivo evaluation of CD44-targeted chondroitin sulphate-conjugated doxorubicin PLGA nanoparticles.  CARBOHYDRATE POLYMERS,      [PMID:30879657] [10.1016/j.carbpol.2019.02.084]

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70. Dong-Xu Tang, Kun Liu, Jin-Yu Yang, Zhi-Jun Wang, Li-Li Fu, Xiao-Jie Yang, Yue-Sheng Li, Bin Huang, Yi Liu.  (2024)  Artificial nonenzymatic antioxidant Prussian blue/KGM-BSA nanocomposite hydrogel dressing as ROS scavenging for diabetic wound healing.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:38552685] [10.1016/j.ijbiomac.2024.131106]

71. Feifei Wang, Dianlong Jia, Qingcui Song, Xiaomeng Lu, Guozi Xia, Jun Li, Xiaodong Jia, Mingliang Gu, Fengjiao Yuan, Zhengping Wang, Zhangyong Xia.  (2025)  Co-delivery of TRAIL and bortezomib via liposomes demonstrates enhanced antitumor efficacy in vivo.  JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.jddst.2025.106602]

72. Rui Sun, Zhongyi Lv, Ying Wang, Menghui Li, Jianrui Qi, Kai Wang, Haihua Yang, Tianli Yue, Yahong Yuan.  (2024)  Different polysaccharide-enhanced probiotic and polyphenol dual-functional factor co-encapsulated microcapsules demonstrate acute colitis alleviation efficacy and food fortification.  CARBOHYDRATE POLYMERS,      [PMID:39227107] [10.1016/j.carbpol.2024.122572]

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74. Su-Yu Yang, Shu-Tong Li, Tian-Hua Li, De-Jin Ma, Jun-Jie Yu, Yang Liu, Yang Yu, Xue-Tao Li, Juan Zang, Zi-Min Yuan, Liang Kong.  (2025)  Dual-Functional Micelles for Codelivery of Sorafenib and Dehydrodiisoeugenol in Treatment of Hepatocellular Carcinoma.  ACS Applied Nano Materials,      [PMID:] [10.1021/acsanm.4c06867]

75. Yuan Wang, Xiaoyu Song, Ru Cui, Xinlin Luo, Juntian Dong, Hanqi Wang, Chengcheng Song, Yifa Zhou, Sisi Cui.  (2024)  Dual-Sensitive Carbohydrate-Based Nanosystem for Targeted Drug Delivery to Potentiate the Therapeutic Efficacy of Ulcerative Colitis.  International Journal of Nanomedicine,      [PMID:39185345] [10.2147/IJN.S472244]

76. Wenbo Yang, Qing Pan, Yizhong Peng, Yuxiang Hu, Yongzhi Cui, Zengwu Shao, Chunqing Meng, Hong Wang, Wei Huang.  (2025)  Dual-target nanotherapy for vascular endothelium and bone mesenchymal stem cells halt steroid-induced osteonecrosis of the femoral head progression.  JOURNAL OF CONTROLLED RELEASE,      [PMID:39761861] [10.1016/j.jconrel.2024.12.081]

77. Dongsheng Ji, Jiahui Zhang, Jihai Liang, Zhi-Shu Huang, Bing Shu, Ding Li.  (2025)  Efficient strategy for alleviating neuronal apoptosis and oxidative stress damage of Alzheimer's disease through dual targeting BCL-2 gene promoter i-motif and β-amyloid.  Redox Biology,      [PMID:40121956] [10.1016/j.redox.2025.103600]

78. Rujing Xu, Ying Lu, Mengxiao Liu, Yanzhao Yin, Junsheng Zhao, Lin Wu, Song Shen.  (2024)  Enhanced Oral Delivery of Insulin via Loading in Polysorbate-80 Micelles.  ACS Applied Nano Materials,      [PMID:] [10.1021/acsanm.4c04262]

79. Shengxia Hu, Dan Sun, Ling Tang, Liang Kong, Yang Liu, Fang Liu, Dongmei Tang, Xuhong Lu, Yuanyuan Wang.  (2025)  Follicle-stimulating hormone peptide-conjugated liposomes in the treatment of epithelial ovarian cancer through the induction of M2-to-M1 macrophage repolarization.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,      [PMID:39933608] [10.1016/j.ijpharm.2025.125334]

80. Xiaomeng Lu, Xinyu Zhan, Guozi Xia, Feifei Wang, Mingjia Lv, Renwei Liu, Yuxue Liu, Chen Zi, Guangyong Li, Rui Wang, Jun Li, Fengjiao Yuan, Dianlong Jia.  (2024)  Improving Targeted Delivery and Antitumor Efficacy of TRAIL through Fusion with a B7H3-Antagonistic Affibody.  MOLECULAR PHARMACEUTICS,      [PMID:39620978] [10.1021/acs.molpharmaceut.4c00891]

81. Chen Yukun, Chen Yuanyuan, Wang Zhenzhi, Yang Lian, Zhang Yu, Zhang Zhanxia, Jia Lijun.  (2024)  Iron-based MOF with Catalase-like activity improves the synergistic therapeutic effect of PDT/ferroptosis/starvation therapy by reversing the tumor hypoxic microenvironment.  JOURNAL OF NANOBIOTECHNOLOGY,  22  (1): (1-18).  [PMID:39543666] [10.1186/s12951-024-02921-7]

82. Xing-Jie Zhang, Yu-Kun Pu, Peng-Yun Yang, Meng-Ru Wang, Rui-Han Zhang, Xiao-Li Li, Wei-Lie Xiao.  (2024)  Isolicoflavonol ameliorates acute liver injury via inhibiting NLRP3 inflammasome activation through boosting Nrf2 signaling in vitro and in vivo.  INTERNATIONAL IMMUNOPHARMACOLOGY,      [PMID:39366075] [10.1016/j.intimp.2024.113233]

83. Xinxi Cai, Aftab Ullah, Rui Qian, Junming Cui, Lin Wu, Song Shen.  (2024)  Membrane-coated protein nanoparticles for mRNA delivery.  JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.jddst.2024.105427]

84. Shutong Li, Suyu Yang, Tianhua Li, Dejin Ma, Yang Yu, Yang Liu, Juan Zang, Liang Kong, Ruibo Guo, Xuetao Li.  (2025)  Modulation of Macrophage Polarization in Rheumatoid Arthritis Therapy via Targeted Delivery of Bulleyaconitine A.  ACS Applied Nano Materials,      [PMID:] [10.1021/acsanm.4c06849]

85. Yuan Gao, Mengyao Mu, Yiju Wei, Bowen Yan, Hui Liu, Kai Guo, Mengmeng Zhang, Xiaohui Dai, Xiao Sun, David Tai Leong.  (2024)  Novel ultrathin ferrous sulfide nanosheets: Towards replacing black phosphorus in anticancer nanotheranostics.  Bioactive Materials,      [PMID:40115876] [10.1016/j.bioactmat.2024.09.035]

86. Guiqi Ma, Xinyu Zhang, Kunlong Zhao, Shuxuan Zhang, Ke Ren, Mengyao Mu, Chenyu Wang, Ximing Wang, Hui Liu, Jian Dong, Xiao Sun.  (2024)  Polydopamine Nanostructure-Enhanced Water Interaction with pH-Responsive Manganese Sulfide Nanoclusters for Tumor Magnetic Resonance Contrast Enhancement and Synergistic Ferroptosis–Photothermal Therapy.  ACS Nano,      [PMID:38251846] [10.1021/acsnano.3c10249]

87. Chaoting Wen, Jialuo Tang, Maowei Wu, Huimin Liu, Xinying Lin, Meidi Fan, Guoyan Liu, Jixian Zhang, Li Liang, Xiaofang Liu, Youdong Li, Yuqing Duan, Xin Xu.  (2025)  Preparation, characterization, and stability of pectin-whey protein isolate-based nanoparticles with mitochondrial targeting ability.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:39880250] [10.1016/j.ijbiomac.2025.140383]

88. Jingmou Yu, Qinglan Yuan, Chuan Li, Sile Hong, Yuting Li, Yafen Li, Jing Ren, Dengzhao Jiang, Pu Chen, Lei Zhang.  (2024)  Reactive oxygen species-sensitive chondroitin sulfate A-cholesteryl hemisuccinate micelles for targeted doxorubicin delivery in tumor therapy.  JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.jddst.2024.105690]

89. Huang Yuanding, Chen Ping, Niu Meng, Peng Weng Kung.  (2024)  Robust and efficient separation of white blood cells from blood using a microfluidic chip with a pair of linearly tapered crossflow filter arrays.  MICROCHIMICA ACTA,  192  (1): (1-12).  [PMID:39738679] [10.1007/s00604-024-06913-0]

90. Jingmou Yu, Mengqi Liu, Chao Zhang, Lizhen Cheng, Changchun Peng, Dengzhao Jiang, Wenbo Liu, Hongguang Jin, Jin Ren.  (2024)  ROS-responsive glycol chitosan-linked prodrug nanoparticle as a nanoplatform for tumor chemo-photodynamic therapy.  PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY,      [PMID:39329286] [10.1080/10837450.2024.2411027]

91. Zihuan Wu, Haiqin Chen, Bo Yang, Jianxin Zhao, Wei Chen.  (2024)  Structural identification and antioxidant activity of trans-9, trans-11, cis-15-conjugated linolenic acid converted by probiotics.  FOOD RESEARCH INTERNATIONAL,      [PMID:38609236] [10.1016/j.foodres.2024.114258]

92. Cong Xu, Jiahui Guo, Baoyue Chang, Qingyun Wang, Yiming Zhang, Xianhui Chen, Wanyi Zhu, Jiage Ma, Shanshan Qian, Zhanmei Jiang, Juncai Hou.  (2024)  Study on encapsulation of Lactobacillus plantarum 23-1 in W/O/W emulsion stabilized by pectin and zein particle complex.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:39242010] [10.1016/j.ijbiomac.2024.135346]

93. Zihan He, Zhangwei Yang, Mingjie Hu, Kexing Wang, Zhenpeng Qiu, Qi Wang, Xinyan Chen, Cong Chang, Junjie Hu, Yan Meng.  (2024)  The β-glucan nanotube carrier achieves detoxification and efficacy enhancement of celastrol in intrahepatic cholangiocarcinoma therapy by increasing targeted controlled release and macrophage polarization.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:39326626] [10.1016/j.ijbiomac.2024.135848]

94. Yang Xin, Cui Limei, Liu Zhonglu, Li Yumei, Wu Xinxin, Tian Ruxian, Jia Chuanliang, Ren Chao, Mou Yakui, Song Xicheng.  (2024)  TMEM16A inhibits autophagy and promotes the invasion of hypopharyngeal squamous cell carcinoma through mTOR pathway.  CARCINOGENESIS,      [PMID:38470063] [10.1093/carcin/bgae020]

95. Yinuo Yuan, Liya Tian, Xinyu Liu, Kai Guo, Ke Ren, Lei Yan, Mengyao Mu, Shangui Liu, Feifei Teng, Cun Liu, Ping Liang, Xiao Sun.  (2025)  Transferrin-Targeted Nanoarchitectonics with Efficient Metastasis Inhibition Enabling Tumor T1-T2 Dual-Mode Magnetic Resonance Contrast Enhancement.  Advanced Healthcare Materials,      [PMID:40693301] [10.1002/adhm.202500690]

96. Lei Huang, Xinbo Li, Hongyan Zhang, Feng Liu, Zheng Dai, Fang Xiao, Lin Wang, Zheng Wang.  (2025)  Zinc Ion-Coordinated Sericin Calcium Phosphate Nanovaccines Induce Hyperactive Dendritic Cells and Synergistic Activation of T Cells for Cancer Immunotherapy.  ACS Nano,      [PMID:40177975] [10.1021/acsnano.4c17491]

97. Ying Lu, Mengxiao Liu, Yanzhao Yin, Junsheng Zhao, Jin Cao, Xueyong Qi, Lin Wu, Song Shen.  (2025)  Self-Hijacking Ferritin Nanostructures for Targeted Delivery of Iron to Enhance Synergistic Anticancer Therapy.  ACS Applied Nano Materials,      [PMID:] [10.1021/acsanm.5c01231]

98. Wu Lihuang, Wang Yuqi, Zhao Xinyue, Zhao Tingting, Li Junhua, Kuang Yi, He Yiyan, Yang Shengxiang, Gu Zhongwei, Mao Hongli.  (2025)  A self-adhesive hierarchical nanofiber patch for dynamic and multistage management of full-thickness cutaneous wounds.  JOURNAL OF NANOBIOTECHNOLOGY,  23  (1): (1-23).  [PMID:40524144] [10.1186/s12951-025-03513-9]

99. Yuanyuan Wang, Ruibo Guo, Baoli Xu, Ming Zou, Liang Kong, Chuan Tang, Runchao Jiang, Wei Wang, Xuan Zhang, Guangyao Lv.  (2025)  Dual-targeted hybrid micelles mediated by VCAM-1 suppress synovial angiogenesis in rheumatoid arthritis.  INTERNATIONAL IMMUNOPHARMACOLOGY,      [PMID:41275827] [10.1016/j.intimp.2025.115910]

100. Pengkai Ma, Ziqi Jing, Xue Wang, Xiaoya Liu, Zirui Tan, Yujie Zhang, Zhijun Wang.  (2025)  Carrier-free multi-components self-delivery nanocomplex for tumor synergistic therapy.  International Journal of Pharmaceutics-X,      [PMID:41323844] [10.1016/j.ijpx.2025.100443]

101. Wen Ou, Na Wen, Huitong Deng, Qianqing Jiang, Dianyi Liu.  (2025)  Antibody-Modified Photovoltaic Biointerface for Neural Stimulation.  ACS Nano,      [PMID:41264884] [10.1021/acsnano.5c12629]

102. Yingqi Wang, Qian Zhang, Bowen Yan, Lihua Pang, Yuhang Ren, Muhammad Inam, Mengmeng Zhang, Nengyi Ni, Xueli Xu, Xiao Sun, Song Zhou.  (2025)  Engineered Gadolinium-Decorated Magnetic Resonance Nanoprobes for Enhanced Liver Fibrosis Theranostics.  Advanced Healthcare Materials,      [PMID:41334670] [10.1002/adhm.202503891]

103. Qian-ni Wu, Zi-ren Feng, Qin Tang, Jiu-ping Jin, Xue-hui Liu, Zhi-bin Mai, Sheng-nan Zhao, Yong-qi Lan, Kai-xin Chen, Jin-duan Lin, Peng-cheng Xu, Ji-jun Fu.  (2025)  Redox-Active Cerium Oxide Nanoparticles Protect Against Acetaminophen-Induced Acute Liver Injury by Modulating Oxidative Stress and Inflammatory Pathways.  MOLECULAR PHARMACEUTICS,      [PMID:41472557] [10.1021/acs.molpharmaceut.5c01647]

104. Ju Ma, Lingling Lv, Yating Guo, Weijie Chen, Ziyu Lin, Xiaoling Qiu, Xiangbin Yu, Yue Yu, Qing Hu.  (2025)  Functionalized Biomimetic Carrier-Free Metal-Coordinated Nanoplatform for Multidimensional Synergistic Therapy of Breast Cancer.  ACS Applied Materials & Interfaces,      [PMID:41453147] [10.1021/acsami.5c20577]

105. Fei Chen, Sisi Wu, Jian Kuang, Sijia Xiang, Shenghu Wang, Siming Yuan, Kaiming Cao, Lu Yu, Wenming Yang, Liqin Tang, Yangzhong Liu.  (2026)  Rosmarinic Acid Is an hCtr1 Inhibitor that Interferes with Copper Transport Activity.  INORGANIC CHEMISTRY,      [PMID:41494012] [10.1021/acs.inorgchem.5c04906]

106. An Chang, Yi Wang, Zan Cui, Yuan-Yuan Liu, Ling-Qi Meng, Jia-Shi, Zhi-Yong Qin, Jia-Ning Gong.  (2026)  Dual-drug nanoplatform HPA@NPs reprograms the fibrotic microenvironment by suppressing EMT and M2 macrophage polarization in pulmonary fibrosis.  INTERNATIONAL IMMUNOPHARMACOLOGY,      [PMID:41679177] [10.1016/j.intimp.2026.116362]

107. Chaoying Kong, Chuwen Luo, Fuxin Xue, Yajun Xu, Na Shen, Zhaohui Tang, Xuesi Chen.  (2026)  Ultrasound-Triggered Charge-Reversal Nanoparticles via Golgi-Dependent Iterative Transcytosis for Enhanced Deep Tumor Penetration.  ACS Nano,      [PMID:41619197] [10.1021/acsnano.5c14557]

108. Xiaojing Chen, Fan Ye, Jianan Song, Shengye You, Xiaobi Fang.  (2026)  Polycatechin nanoparticle-enhanced hydrogel as a multifunctional platform for targeted diabetic oral ulcer repair.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2026.140828]