Hsp70 Mouse mAb - Primary antibody, specific to HSPA1A, Mouse IgG2b

Cat. No.: Ab108897
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. See COA
Application
IF/ICC, IHC, WB
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG2b
Clone Number
2#
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab108897-10μl
4
$99.90
50μl
Ab108897-50μl
4
$259.90
100μl
Ab108897-100μl
3
$419.90
1ml
Ab108897-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$2,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human Hsp70 Antibody, Monoclonal (2#), could be used for WB, IHC, ICC, IF and so on.

Application:

WB: 0.01-3µg/mL IHC: 5-20µg/mL ICC/IF: 5-20µg/mL

Protein Function

In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.Tissue specificity: HSPA1B is testis-specific.

Specifications

Product Name
Hsp70 Mouse mAb - Primary antibody, specific to HSPA1A, Mouse IgG2b
Synonyms
DnaK type molecular chaperone HSP70 1 antibody | Epididymis secretory protein Li 103 antibody | FLJ54303 antibody | FLJ54370 antibody | FLJ54392 antibody | FLJ54408 antibody | FLJ75127 antibody | Heat shock 70 kDa protein 1 antibody | Heat shock 70 kDa pr
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species
Mouse
Specificity
HSPA1A
Immunogen
Recombinant HSPA1A expressed in E.coli (AA 1-641)
Positive Control
WB: Hep G2, HeLa, Jurkat and A549 cell lysates. IHC: Human breast cancer, prostate tissues. ICC/IF: Hep G2 cells.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotype
Mouse IgG2b
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Hsp70 Mouse mAb (Ab108897) - Western Blot
All lanes: Hsp70 Mouse mAb (Ab108897) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 0.8 s

Hsp70 Mouse mAb (Ab108897) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 cell
Primary Ab: 20 μg/mL Hsp70 Mouse mAb (Ab108897)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Hsp70 Mouse mAb (Ab108897) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 20 µg/mL Hsp70 Mouse mAb (Ab108897)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Hsp70 Mouse mAb (Ab108897) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 20 µg/mL Hsp70 Mouse mAb (Ab108897)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application
ApplicationDilution info
WB

0.01-3 µg/mL

IHC

5-20 µg/mL

IF/ICC

5-20 µg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
HSPA1A Tchem Heat shock 70 kDa protein 1A (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
HSPA1A Tchem Heat shock 70 kDa protein 1 (164 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404796Certificate of AnalysisFeb 10, 2026 Ab108897
ZJ23F1101839Certificate of AnalysisNov 29, 2023 Ab108897
ZJ23F1101840Certificate of AnalysisNov 29, 2023 Ab108897
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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