Human Mitofusin 2 (MFN2) ELISA Kit

Cat. No.: EJ1514542

GRADE & PURITY BioReagent

Panoramica

This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MFN2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MFN2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.

Specifications

Specifiche e purezza

BioReagent

Grado

BioReagent

Applicazione

ELISA

Tipo di campione

Lisato cellulare, omogenati tissutali, supernatanti di colture cellulari

Campo di rilevamento

0.156ng/mL~10ng/mL

Specie rilevata Oggetto

Human

Specificità

Detecting Human MFN2 in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Store at 2-8℃ for 6 months.