GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Hydroxyproline (4‑hydroxyproline, Hyp) is a non‑essential amino acid and one of the major components of collagen. In mammals, Hyp is found almost exclusively in collagen and elastin, whereas in plants, it is widely distributed in various proteins. Numerous diseases associated with abnormal collagen metabolism can lead to alterations in Hyp levels in blood, urine, and tissues. Therefore, Hyp level is an important reference indicator for the clinical evaluation of collagen metabolism‑related disorders.
This kit employs a colorimetric method for the determination of Hyp content. After acid hydrolysis, free Hyp is released from the sample. The free Hyp is oxidized by Chloramine T, and the oxidation product reacts in the presence of p‑dimethylaminobenzaldehyde (DMAB) to form a purple‑red compound, which exhibits a characteristic absorption peak at 560 nm. By measuring the absorbance of the reaction solution at 560 nm (which is directly proportional to the Hyp content), the hydroxyproline concentration in the sample can be quantitatively calculated.
Specifications
Specifiche e purezza
BioReagent
Grado
BioReagent
Detection instrument
Visible Spectrophotometer
Detection wavelength
560nm
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at 2-8°C
Spedito in
Wet ice
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
