Hygromycin B is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus. Its mechanism of action involves interference with the translocation process of the 70S ribosome and induction of misreading of the mRNA template, thereby inhibiting protein synthesis and ultimately leading to the death of both prokaryotic cells (e.g., bacteria) and eukaryotic cells (including yeasts, fungi, and higher mammalian cells).
In the laboratory, Hygromycin B is widely used for the selection and maintenance of prokaryotic or eukaryotic cells harboring the hygromycin resistance gene. This resistance gene encodes a hygromycin kinase that inactivates Hygromycin B through phosphorylation. Since the discovery of the hygromycin resistance gene, Hygromycin B has become a standard selection reagent in transgenic experiments. It is particularly suitable for plant tissue culture and can be used in combination with other selective agents to establish dual‑selection systems.
Instructions for Use
This product is a sterile Hygromycin B solution and can be diluted directly for use.
Working concentrations: 50‑1000 µg/mL for cell culture. A typical working concentration for mammalian cell culture is 200 µg/mL; 20‑200 μg/mL for bacteria and plant cells; 200‑1000 μg/mL for fungi. However, the optimal working concentration should be determined empirically through preliminary titration experiments, as it varies depending on the specific cell type, culture medium composition, and experimental system.
Precautions
1.This product is a toxic compound. Avoid contact with skin and eyes; handle with care.
2.Wear a lab coat and disposable gloves for personal safety and health during operation.
