I-A/I-E Rat mAb (APC) - Anticorpo primario, specific to I-A/I-E, Rat IgG2b

Cat. No.: Ab190261

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

consiglio

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

M5/114

Conjugation

Unconjugated

Panoramica

Rat anti Mouse I-A/I-E Antibody, Monoclonal (M5/114), could be used for Flow.

Specifications

Product Name

I-A/I-E Rat mAb (APC) - Anticorpo primario, specific to I-A/I-E, Rat IgG2b

Sinonimi

IAb | Ia2 | Ia-2 | Abeta | H-2Ab | H2-Ab | Rmcs1 | I-Abeta | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | E-alpha-f | H-2 class II histocompatibility antigen, E-K alpha chain | H-2 class II histocompatibility antigen, E-U alpha chain | H-2E | H2-Ea | H-2Ea | H2-Ea-ps

Specifiche e purezza

ExactAb™, Validated, 0.5 mg/mL

Meccanismi biochimici e fisiologici

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides

Specie ospite

consiglio

Specificità

I-A/I-E

Immunogeno

Activated C57BL/6 mouse spleen cells

Controllo positivo

Flow: C57BL/6 mouse splenocytes.

Coniugazione

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Rat IgG2b

Tipo di catena leggera

kappa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: I-A/I-E Rat mAb (APC) (Ab190261)

I-A/I-E Rat mAb (APC) (Ab190261) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with I-A/I-E Rat mAb (APC) (Ab190261) at 0.012μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (APC) (Ab180313).

Flow Cytometry: I-A/I-E Rat mAb (APC) (Ab190261)

I-A/I-E Rat mAb (APC) (Ab190261) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with I-A/I-E Rat mAb (APC) (Ab190261) at 0.012μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  ≤0.1 μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.;

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (I-A/I-E Rat mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.