GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Suitable for mass spectrometry (MS)?MS grade — low non-volatile and ionic residues compatible with mass spectrometry. Use to minimize background and ion suppression in MS workflows.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥99%(HPLC)≥50 U/μg protein
EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),≥50 U/μg protein Adatto alla spettrometria di massa (MS),Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
IdeZ (immunoglobulin IgG-specific degrading enzyme) is an engineered protease recombinantly expressed in Escherichia coli.
It specifically cleaves IgG at a single recognition site below the hinge region to generate homogeneous F(ab´)₂ and Fc fragments.
Both IdeS and IdeZ proteases can efficiently cleave human IgG1, IgG2, IgG3 and IgG4, monkey, sheep, rabbit, humanized and chimeric IgG, as well as Fc fusion proteins.
IdeZ protease can cleave mouse IgG2a and IgG3.
Reconstitution:
Reconstitute with 40 μL of ultrapure water.
Digestion Buffer:
10× buffer containing 50 mM PBS, 150 mM NaCl (pH 6.6).
pH Range:
Optimal activity at pH 6–8.
Application:
IdeZ protease is an IgG-specific degrading enzyme that generates homogeneous F(ab´)₂ and Fc fragments.
Recommended Digestion SOP:
· Take 10 μL of mAb sample at 10 μg/μL;
· Add 5 μL of 10× digestion buffer and 31 μL of ultrapure water, mix to a total reaction volume of 50 μL;
· Add 4 μL of IdeZ protease at 0.5 μg/μL;
· Incubate at 37 °C for 30 minutes.
Specifications
Product Name
IdeZ Protease (MS)
Grado
Adatto alla spettrometria di massa (MS), Recombinant, EnzymoPure™
Specifiche e purezza
EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),≥50 U/μg protein
Tipo di molecola
Enzima
Stoccaggio e spedizione
Concentrazione
≥50 U/μg protein
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Definizione dell'unità
The amount of IdeZ protease required to completely cleave 50 μg of IgG antibody within 30 minutes at 37 °C.
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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