IDH1 Mouse mAb - Anticorpo primario, specific to IDH1, Mouse IgG2a

Cat. No.: Ab109324

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, See COA

Application

IHC, WB

Host species

Mouse

Reactive species

Mouse, Umano

Isotype

Mouse IgG2a

Clone Number

C12

Conjugation

Unconjugated

Panoramica

Mouse anti Human IDH1 Antibody, Monoclonal antibody (C12), could be used for WB,IHC and so on.
Application:
WB: 0.01-2µg/mL
IHC:5-30µg/mL
Protein Function

Specifications

Product Name

IDH1 Mouse mAb - Anticorpo primario, specific to IDH1, Mouse IgG2a

Sinonimi

Cytosolic NADP isocitrate dehydrogenase antibody | Cytosolic NADP-isocitrate dehydrogenase antibody | Epididymis luminal protein 216 antibody | Epididymis secretory protein Li 26 antibody | HEL-216 antibody | HEL-S-26 antibody | ICDH antibody | IDCD antib

Specifiche e purezza

Carrier Free, ExactAb™, Validated, High Performance, See COA

Specie ospite

Mouse

Specificità

IDH1

Immunogeno

Recombinant IDH1 expressed in E.coli (AA 74-333).

Controllo positivo

WB: Hep G2 and NIH/3T3 cell lysates. IHC: Human colorectal cancer, stomach, kidney, prostate, glioma tissues.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Mouse IgG2a

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A + Protein G purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - Western Blot
All lanes: IDH1 Mouse mAb (Ab109324) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 47 kDa
Observed band size: 44 kDa
Exposure time: 25.4 s

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Colorectal cancer Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Stomach Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Glioma Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Applicazione

• Applicazione: Dilution info
• IHC:

  5-30 µg/mL;

• WB:

  0.01-2 µg/mL ;

Obiettivi associati (umani)

IDH1 Tclin Isocitrate dehydrogenase [NADP] cytoplasmic (0 Activities)

Discover Target: IDH1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

IDH1 Tclin Isocitrate dehydrogenase [NADP] cytoplasmic (40980 Activities)

Discover Target: IDH1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IDH1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.