IL-6 Mouse mAb - Anticorpo primario, specific to Human IL-6

Cat. No.: Ab188014

QUALITÀ & PUREZZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Capture Antibody, ELISA

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

C05/5G3

Conjugation

Unconjugated

Panoramica

Mouse anti Human IL-6 Antibody, clone C05/5G3, could be used for a Capture Antibody in sandwich ELISAs

Specifiche

Product Name

IL-6 Mouse mAb - Anticorpo primario, specific to Human IL-6

Sinonimi

Interleukin BSF 2 antibody | B cell differentiation factor antibody | B cell stimulatory factor 2 antibody | B-cell stimulatory factor 2 antibody | BSF 2 antibody | BSF-2 antibody | BSF2 antibody | CDF antibody | CTL differentiation factor antibody | Cyto

Specifiche e purezza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Meccanismi biochimici e fisiologici

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induc

Specie ospite

Mouse

Specificità

Human IL-6

Immunogeno

Recombinant protein corresponding to Human IL-6

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Coppie abbinate

[Ab168996] IL-6 Mouse mAb, Detection Antibody

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Immagini

Sandwich ELISA - IL-6 Antibody Pair (Ab188014 & Ab168996)

Sandwich ELISA - IL-6 standard curve
Sandwich ELISA detection of Recombinant Human IL-6 Protein (rp156005) using IL-6 Mouse mAb (Ab188014) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-6 Mouse mAb (Ab168996) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Detection range: 10.0 ng/mL - 10000.0 ng/mL

Indirect ELISA - IL-6 Mouse mAb (Ab188014)

Indirect ELISA - IL-6 standard curve
Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with IL-6 Mouse mAb (Ab188014). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.

EC₅₀: 30.23 ng/mL

Applicazione

Applicazione

Dilution info

ELISA

1.0 µg/mL as a Capture antibody in ELISA

Obiettivi associati (umani)

IL6 Tclin Interleukin-6 (0 Activities)

Discover Target: IL6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IL-6 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.