Imidazole buffer Solution - UltraBio™, Ultra pure, 1 M in H2O , CAS No.288-32-4

CAS: 288-32-4 Cat. No.: I432545 Formula: C3H4N2 Peso molecolare: 68.08 Beilstein Registry Number: 103853 Numero EC: 206-019-2
Disponibile su ordine
GRADE & PURITY UltraBio™ ? UltraBio™ — Aladdin's line for molecular-biology applications. Use for nuclease-free, high-consistency reagents across molecular workflows. Ultra pure ? Ultra-pure grade with very low impurity content across the board. Use for trace analysis, electronics, or processes intolerant of contamination. 1 M in H2O
Synonyms
CHEBI:14434 | 1H-Imidazole | BRN 0103853 | SILDENAFIL CITRATE IMPURITY E [EP IMPURITY] | BDBM7882 | I0001 | Imidazole, ReagentPlus(R), 99% | FLUTRIMAZOLE IMPURITY A [EP IMPURITY] | Formamidine,N'-vinylene- | Glyoxaline solution | Him | NSC-60522 | UNII-7G
Storage
Room temperature
★
Size
Germania (EU)
USA*
Price
Qty
100ml
I432545-100ml
Su ordinazione · 8–12 settimane
86,69€
500ml
I432545-500ml
Su ordinazione · 8–12 settimane
277,59€
Enter a quantity for the sizes you want to add.
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Why this grade

UltraBio™, Ultra pure, 1 M in H2O Ultra pure,UltraBio™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Room temperature Ships Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 429 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Panoramica

Application

Imidazole can be used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins ( P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Specifications

Sinonimi
CHEBI:14434 | 1H-Imidazole | BRN 0103853 | SILDENAFIL CITRATE IMPURITY E [EP IMPURITY] | BDBM7882 | I0001 | Imidazole, ReagentPlus(R), 99% | FLUTRIMAZOLE IMPURITY A [EP IMPURITY] | Formamidine,N'-vinylene- | Glyoxaline solution | Him | NSC-60522 | UNII-7G
Specifiche e purezza
UltraBio™, Ultra pure, 1 M in H2O
Condizioni di conservazione di stoccaggio
Room temperature
Grado
Ultra pure, UltraBio™
Nomi e identificatori
Sorrisi canonici
C1=CN=CN1
IUPAC Name
1H-imidazole
InChIKey
RAXXELZNTBOGNW-UHFFFAOYSA-N
INCHI
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
Isomeri SMILES
C1=CN=CN1
WGK Germania
1
RTECS
NI3325000
Numero UN
2923
Peso molecolare
68.08
Beilstein
103853
Reaxy-Rn
103853
Reaxys-RN_link_address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=103853&ln=

Documentazione

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassOrganoheterocyclic compounds
ClasseAzoles
SubclassImidazoles
Intermediate Tree Nodes Not available
Direct ParentImidazoles
Alternative Parents Heteroaromatic compounds  Azacyclic compounds  Organopnictogen compounds  Organonitrogen compounds  Hydrocarbon derivatives  
Molecular FrameworkAromatic heteromonocyclic compounds
Substituents Heteroaromatic compound - Imidazole - Azacycle - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Organonitrogen compound - Aromatic heteromonocyclic compound
DescrizioneThis compound belongs to the class of organic compounds known as imidazoles. These are compounds containing an imidazole ring, which is an aromatic five-member ring with two nitrogen atoms at positions 1 and 3, and three carbon atoms.
External Descriptors an aromatic compound
Struttura 3D
Modello di struttura chimica interattiva





Obiettivi associati (umani)
EPHX1 Tchem Epoxide hydrolase 1 (644 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
TBXAS1 Tchem Thromboxane-A synthase (3355 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CYP1B1 Tchem Cytochrome P450 1B1 (1148 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
NOS1 Tchem Nitric-oxide synthase, brain (1786 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ALDH1A1 Tchem Aldehyde dehydrogenase 1A1 (77053 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PTGIS Tchem Prostaglandin I2 synthase (104 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CYP3A4 Tclin Cytochrome P450 3A4 (53859 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HL-60 (67320 Activities)
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MCF7 (126967 Activities)
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SW480 (6023 Activities)
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A549 (127892 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Lymphoblastoid cell (5959 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
QPCT Tchem Glutaminyl-peptide cyclotransferase (1121 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SMMC-7721 (5516 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
APEX1 Tchem DNA-(apurinic or apyrimidinic site) lyase (38016 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
FKBP5 Tchem Peptidyl-prolyl cis-trans isomerase FKBP5 (259 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Obiettivi associati (non umani)
Nos2 Nitric oxide synthase, inducible (3573 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Saccharomyces cerevisiae (19171 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Plasmodium falciparum (966862 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CHO (4503 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Rorc Nuclear receptor ROR-gamma (89407 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Meccanismi d'azione
Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Proprietà chimiche e fisiche
Punto di infiammabilità (°C)145℃
Punto di ebollizione (°C)257°C
Punto di fusione (°C)90-91°C
Peso molecolare68.080 g/mol
XLogP3-0.100
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass68.0374 Da
Monoisotopic Mass68.0374 Da
Topological Polar Surface Area28.700 Ų
Heavy Atom Count5
Formal Charge0
Complexity28.100
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count1
Domande frequenti e articoli
UltraBio™: Defining Excellence in Molecular Biology Applications
Comparing Guanidine Hydrochloride and Urea for Inclusion-Body Solubilization
Why Did Nickel Agarose Beads Lose Their Color?
Why Did My Ni-Agarose Beads Turn Black or Brown?
How to Optimize Buffer Components for Ni-Agarose Beads
Stripping, Cleaning, and Metal Recharging Workflow for Ni²⁺–Agarose Resins
Obtaining soluble and biologically active recombinant proteins from inclusion bodies
How to Choose the Best Protein Purification Technique
His-Tagged Protein Purification
His-Tag Protein Purification: Common Troubleshooting Questions and Practical Solutions
Scientific Decision-Making for Chromatography Buffer Selection: Tris-HCl vs Phosphate Systems
OSF/ASF Dual-Channel Toolbox: Divergent Reactions of Four-Membered Ring Sulfonyl Fluorides, Condition “Knobs,” and Selection Navigation
Protein Tags Demystified: Classification, Properties, and Suitable Experimental Applications
Why Copper Develops Local Pinholes in Water Systems: The Film-Forming Protection Mechanism of Benzotriazole (BTA), Key Variables, and a Selection Guide (Tables 1–3)
Tuning Logic of Imidazole Heteroaromatic Building Blocks: How Dual Nitrogen Sites and Substitution / Salt Forms / Ring Fusion Modulate Charge, Hydrogen Bonding, and Coordination (with Tables A–E)
Invertebrate Oxygen Carrier Proteins in Immunology and Vaccine Research
Silicon Reagent-Assisted Peptide Synthesis: Research Progress and Methodological Value of Peptide Bond Formation from Unprotected Substrates
Decision Logic for Peptide Synthesis Routes: Applicability and Criteria for Choosing among SPPS, LPPS, and Recombinant Expression
DMAP Is More Than Just an Organic Base: Experimental Judgment from Nucleophilic Acyl Transfer to Esterification and Macrolactonization
Experimental Selection of SEM-Cl: From O/N-SEM Protection and Directed Functionalization of N-Heterocycles to the Introduction of Protected C1 Fragments
4-[(tert-Butyldimethylsilyl)oxy]butan-1-ol: Experimental Selection Logic as a Monoprotected 1,4-Butanediol Building Block and a Protected C4 Linker
Formulation Design and Selection of Amine Curing Agents in Epoxy Systems
Protein Structure Analysis: An Experimental Path from Defining the Question to Functional Validation
Understanding Amine Curing Agents: Structure, Types, and Application Selection
Adding a Temperature-Triggered Switch to NCO: How Blocked Isocyanates Affect the Storage, Curing, and Film Performance of 1K Baking Coatings
Epoxy Phenolic Coatings: Why Are They Commonly Used for Chemical Resistance, Solvent Resistance, Heat Resistance, and Internal Metal Surface Protection?
What Is Synthetic Biology? The Industrialization Pathway from Upstream Reagent Toolchains to Biomanufacturing Applications
Why Is the meta-Fluoropyridine Motif Difficult to Make? Understanding the Synthetic Logic of Stable Enamine Building Blocks from the Risks of the Balz–Schiemann Reaction
Structural Features, Property Modulation, and Typical Applications of Triazole Compounds
Synthetic Application Logic of Triazoles and Related Compounds: Structural Introduction, Reactive Sites, and Target Molecule Construction
One-Pot Arylation of the Piperidine α-C–H Bond: From Directed Lithiation and Li→Zn Transmetalation to Negishi Coupling
Overcoming the Electronic-Effect Limitations of Nucleophilic Aromatic Substitution in Fluoroarenes: Anion Activation, Concerted Substitution, and HF Capture by t-Bu-P4
What Is “Plant Collagen”? Distinguishing Ingredient Types, Structural Differences, Production Methods, and Skincare Effects
From 459,000 Recorded Ring Systems to 3,902 Candidate Ring Systems: Selection Patterns of Clinically Relevant Ring Systems
Citations of This Product
Calcolatori di soluzioni
Recensioni

Recensioni dei clienti

Domande frequenti

What does UltraBio? mean?
UltraBio? indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at room temperature.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 288-32-4, the molecular formula is C3H4N2, and the molecular weight is 68.08 g/mol. InChIKey RAXXELZNTBOGNW-UHFFFAOYSA-N.
What does Ultra pure mean?
Ultra pure is a defined analytical purity tier suitable for general analytical and reagent applications. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Ultra pure is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

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