IRF5-IN-1 (compound C5) is an IRF5 pathway-specific inhibitor. IRF5-IN-1 significantly reduces IRF5 nuclear translocation without affecting the activation of NF-κB p65. IRF5-IN-1 acts through SLC15A4 to selectively inhibit TLR7/8-induced IRF5 responses in cells.

Specifiche

Specifiche e purezza

Moligand™, 10 mM in DMSO

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

Moligand™

Nomi e identificatori

Peso molecolare

431.57

Dati avanzati

Certificati (CoA, COO, BSE/TSE e tabella di analisi)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Calcolatori di soluzioni

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Recensioni

Application Protocols

Tested applications, recommended concentrations/dilutions, and positive controls: Not specified for this item; refer to CoA/Spec Sheet or technical note if available.

General guidance for small-molecule screening (non-item-specific):

Stock preparation: Prepare a 10–50 mM stock in DMSO, filter (0.2 µm PTFE), and aliquot to avoid freeze–thaw cycles.
Biochemical assays: Titrate compound across a logarithmic concentration range (e.g., 10-point 1:3 serial dilution). Keep final DMSO constant across all wells.
Cell-based assays: Verify cytotoxicity windows (e.g., resazurin/CellTiter-type assay) and perform time-course to distinguish acute vs transcriptional effects.
Orthogonal confirmation: Use a secondary readout (e.g., reporter assay, qPCR of target genes, CETSA/DSF) to verify mechanism.

These are general practices and are not validated specifically for this item.

Biological Roles

Item-specific biological target/role: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general context (for orientation only):

The acronym IRF commonly refers to interferon regulatory factors, a family of transcription factors involved in pattern-recognition receptor signaling and innate immune gene expression. IRF5, in particular, is implicated in the regulation of type I interferon and proinflammatory cytokine transcription in response to upstream signals (e.g., TLR–MyD88 pathways).
Small molecules described as “IRF5 inhibitors” in literature are often used to interrogate transcriptional programs, dissect upstream signaling crosstalk, and explore structure–activity relationships affecting nuclear translocation, dimerization, DNA binding, or cofactor recruitment.

Practical considerations (general):

When evaluating modulators of transcription factors, include appropriate reporter assays, qPCR of target genes, and orthogonal target engagement methods (e.g., CETSA or ChIP-based readouts) to confirm on-target activity.
Control for cytotoxicity and off-target effects via cell viability assays and counter-screens against related pathways.

Note: The above is general biological background and does not constitute a specification or claim for this particular item. Consult primary data and the item’s CoA to verify target relevance.

Buffer Applications

This product is a small-molecule screening compound, not a buffering agent. Therefore, standard buffer system recipes (e.g., phosphate, HEPES, Tris) are not directly applicable to the compound itself.

Practical handling guidance (general):

Prepare a concentrated stock in DMSO (if compatible), then dilute into the assay buffer of choice (PBS, HEPES, or cell culture media) with vigorous mixing to avoid precipitation.
Maintain a consistent final solvent percentage across all wells/samples to avoid solvent-driven artifacts.

Item-specific buffer compatibility or pH stability: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

As a small-molecule screening compound, IRF5-IN-1 itself is not a process solvent or reagent for which green replacements typically apply. Greenness considerations focus on handling solvents and assay media.

Greener handling choices (general guidance):

Prefer water-based buffers with minimal DMSO content where feasible, consistent with assay requirements.
When a nonaqueous solvent is necessary, consider ethanol as a more benign alternative to DMF, provided solubility and assay tolerance permit.
Reduce solvent volumes by preparing higher-concentration DMSO stocks and performing precise microdilutions.

Comparison (general; literature):

DMSO vs Ethanol: DMSO offers broader solubility and lower volatility but is less biodegradable; ethanol is renewable and greener but provides lower solvency for many hydrophobic ligands.
DMF: Avoid in biological assays when possible due to toxicity and EHS concerns; if required, use minimal volumes and capture waste appropriately.

Operational sustainability:

Consolidate thaw/freeze cycles to limit material waste.
Use amber vials or light-protective wraps to avoid photodegradation and reduce the need for repeated preparations.
Segregate halogenated vs non-halogenated waste; keep aqueous DMSO waste separate to facilitate downstream treatment.

Pharmaceutical Uses

This material is supplied strictly for research use only and is not intended for human or veterinary use, nor for diagnostic or therapeutic applications.

Item-specific pharmacopeial status, excipient role, or GMP suitability: Not specified for this item; refer to CoA/Spec Sheet.

General formulation context for research compounds:

For in vitro studies, DMSO stock solutions are commonly diluted into aqueous buffers or cell culture media. Confirm precipitation risk and assay tolerance to solvent.
For exploratory in vivo research (if permitted under institutional policies), vehicles may include aqueous solutions with biocompatible cosolvents/surfactants (e.g., PEG400, Captisol, tween, or cremophor systems). Such use requires full structural information and stability data; none are provided here.
No clinical claims, dosage forms, or regulatory submissions are associated with this listing.

Physical Properties

Item-specific properties:

Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
Melting point, boiling point, density, refractive index, logP, pKa, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.
Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance for screening compounds (literature/general):

Many discovery-stage small molecules are supplied as solids or as DMSO stocks. If solid, typical first-line solvents for reconstitution are DMSO, DMF, ethanol, or aqueous buffer with co-solvent, selected after a small-scale solubility screen.
A pragmatic workflow: perform a 1–2 mg solubility scout across DMSO and ethanol (room temperature), then across PBS or assay buffer with 1–2% DMSO. Use visual clarity and LC/UV to confirm.
If the compound is sparingly soluble, gentle warming (≤40 °C), sonication, or stepwise co-solvent addition can help. Avoid strong base/acid adjustments unless the ionizable groups are known and compatible with the assay.

Note: Do not treat the above as specifications. For exact property values and solvent recommendations applicable to this specific item, consult the CoA/Spec Sheet.

Quality and Grades

Item-specific facts:

Grade: Moligand
Purity: Not specified for this item; refer to CoA/Spec Sheet.

What Moligand grade typically entails (general description):

Moligand indicates inclusion in a curated small-molecule/ligand collection designed for screening, target validation, or chem-bio probe development. Such lines commonly emphasize confirmed identity (e.g., LC-MS, 1H NMR) and research-ready form.
While high purity is usually targeted for screening libraries to minimize assay artifacts, explicit specifications (percent purity, residual solvent levels, metal content, or stabilizers) are not provided here and must be verified on the CoA.

Analytical documentation and verification:

For method development or regulatory-facing work, request: certificate of analysis (purity by HPLC/UPLC, LC-MS, NMR), residual solvent data, water content (Karl Fischer), and any stabilizer/additive information.
If using in quantitative assays, obtain extinction coefficients (if UV-active) and stability data in your assay matrices or vehicles.

Handling implications:

If no stabilizer is listed, assume none is added and minimize exposure to air/light until stability is known. Filter solutions (0.2 µm PTFE) prior to critical assays to remove particulates and ensure baseline stability.

Reaction and Applications

This catalog entry is part of a small-molecule/ligand library intended for biological screening and chemical biology studies rather than as a synthetic reagent.

Item-specific functional reactivity or application notes: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance for screening compounds:

Use in target engagement assays (e.g., reporter gene assays, transcription factor activity assays, thermal shift/DSF, CETSA) after confirming solubility and stability in assay buffers.
For biochemical assays, determine DMSO tolerance of the enzyme/cellular system and maintain constant DMSO across controls and treatments.
Evaluate compound stability under assay conditions (time, temperature, light) via LC-MS or HPLC to ensure the active entity is present throughout the readout window.
If mechanism involves transcriptional regulation, time-course and washout studies can help distinguish primary from downstream effects in cell-based systems.

Note: If you intend to use this material as a synthetic intermediate, request full structural information and assess compatibility with planned transformations; otherwise, treat primarily as a probe candidate for research use.

Reaction Conditions

Not typically applicable. This product is not supplied as a synthetic reagent, and no reaction condition data are provided.

Item-specific conditions (solvent, temperature, catalysts): Not specified for this item; refer to CoA/Spec Sheet.

General laboratory handling conditions:

For solution preparation, use anhydrous DMSO or ethanol as needed and work at ambient temperature unless stability indicates cooling.
Protect from light and moisture until solution stability is characterized.

If you intend to employ this compound in chemical reactions, obtain the full structural information first and verify compatibility with intended reagents and conditions.

Safety and Handling

Item-specific hazard classification: Not specified for this item; refer to SDS.

Signal Word: Not specified for this item; refer to SDS.
H-Statements: Not specified for this item; refer to SDS.
GHS Classification/Pictograms: Not specified for this item; refer to SDS.

General laboratory safety (best practice):

PPE: lab coat, safety glasses, and appropriate chemically resistant gloves (e.g., nitrile). Work in a fume hood to avoid inhalation of dust or vapor.
Avoid contact with skin and eyes. Prevent aerosol generation when handling powders. Use secondary containment for cryogenic shipments.
Incompatibilities: Without structure-specific data, avoid strong oxidizers and extreme pH until compatibility is known. Keep away from moisture if hygroscopicity is unknown.
First-aid (overview): If inhaled, move to fresh air; in case of skin/eye contact, rinse cautiously with water for several minutes; if ingested, rinse mouth and seek medical attention. Always follow site SOPs and SDS guidance.
Spill/cleanup: Capture solids with damp disposable towels or HEPA vacuum suitable for fine powders; contain liquids with inert absorbent. Dispose in accordance with institutional and local regulations.

Special notes:

Product ships on dry ice/cold packs and is stored at −80 °C. Handle frozen vials with cryogenic gloves; avoid thermal shock. Thaw on ice when feasible.
Always consult the product-specific SDS for authoritative hazard and response measures.

Solvent Selection

Item-specific solubility and polarity: Not specified for this item; refer to CoA/Spec Sheet.

General guidance for reconstitution and working solutions (literature/general):

Primary solvent: DMSO is the default for small-molecule screening stocks due to broad solvating power and biological assay compatibility at low percentages (typically ≤0.5–1% v/v in final wells). Prepare a 10–50 mM master stock when solubility permits.
Secondary options: DMF and ethanol can be useful cosolvents. For aqueous delivery, pre-dissolve in DMSO, then dilute into buffer (e.g., PBS, HEPES) with vigorous mixing to avoid precipitation. Maintain final DMSO ≤1% unless your assay tolerates more.
If compound is hydrophilic (once known), direct dissolution in water or buffer may be possible; adjust pH only if ionization behavior is characterized.

Comparison snapshot (general):

DMSO: highest solvency; broad assay compatibility; hygroscopic; can affect some enzymes at >1%.
Ethanol: volatile; limited aqueous compatibility at higher fractions; may perturb membranes.
DMF: strong solvent; more toxic; less preferred in cell-based assays.

Practical tips:

Conduct a mini solubility screen (DMSO, ethanol, buffer + 1–2% DMSO) at target concentrations. Inspect for clarity and perform LC to confirm concentration.
Filter working solutions through 0.2 µm PTFE or PVDF to remove particulates.
Record exact solvent composition in assay notebooks for reproducibility.

Storage and Reconstitution

Item-specific storage and shipping:

Storage Conditions: Store at −80 °C
Shipped In: Dry ice packs + Cold packs

Additional item-specific details (appearance, solvent, stabilizer): Not specified for this item; refer to CoA/Spec Sheet.

General reconstitution guidance (non-item-specific):

If supplied as a solid: Equilibrate to room temperature in a desiccator before opening to avoid moisture condensation. Dissolve in anhydrous DMSO to prepare a 10–50 mM stock, subject to solubility. Vortex/sonicate gently to aid dissolution.
If supplied as a solution: Keep frozen at −80 °C. Thaw on ice, mix gently, and minimize time at room temperature. Do not refreeze thawed master vials; aliquot immediately.
Aliquoting: Dispense single-use aliquots (e.g., 10–50 µL) in inert, low-bind, amber vials or PCR strips. Avoid repeated freeze–thaw cycles.
Light and moisture protection: Use amber containers or wrap in foil. Work under dry atmosphere if hygroscopicity is suspected.
Working solution stability: Confirm by LC/UV or LC-MS over the assay window. Record solvent composition, concentration, and preparation date.

Disclaimers:

Exact solubility, pH stability, and long-term stability are not specified for this item; consult the CoA/Spec Sheet and SDS for definitive instructions.

Structure and Identity

Brief overview: IRF5-IN-1 is listed in our Moligand small-molecule collection. Item-specific structural identifiers are not provided in the current record.

Item-specific facts (from Product Data):

SKU: I1497619
Product Name: IRF5-IN-1
CAS: 689270-18-6
Grade: Moligand
Storage: Store at -80°C
Shipment: Dry ice packs + Cold packs
Research use: For research use only
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

General/literature context (not item-specific):

Naming convention “IN-1” is commonly used in literature to denote a first-in-series small-molecule inhibitor against a target (here, presumably IRF5), but this has not been confirmed for this specific catalog item. Please verify with the CoA or supporting documentation.

Structural features (descriptive):

2D structure, ring systems, heteroatoms, and functional groups are not disclosed in this entry. If needed for computational work or method development, request the full chemical structure from the CoA/Spec Sheet or supplier technical support.

Synthetic Utility

This catalog entry is positioned as a screening/ligand compound rather than a building block or reagent. Without structural disclosure, synthetic transformation guidance is not applicable.

Item-specific functional groups or reactivity: Not specified for this item; refer to CoA/Spec Sheet.

General notes (if the structure becomes available):

If future workflows require derivatization (e.g., for probe conjugation or SAR expansion), identify functional handles (amines, phenols, carboxylates, halides) and assess stability to common coupling conditions (amide coupling, Suzuki, click chemistry).
For bioconjugation or immobilization, consider introducing linkers via robust reactions (e.g., NHS ester formation from carboxylates, CuAAC from alkyne/azide) while monitoring retention of bioactivity.

Until the full structure is confirmed, treat this material strictly as a finished test article rather than a synthetic intermediate.

Target Specificity

Only item-specific data may be listed here.

Molecular target, binding site/epitope, potency (IC50/Ki), species selectivity, and off-target profile: Not specified for this item; refer to CoA/Spec Sheet and any available technical datasheet.

Note: The product name suggests a potential connection to IRF5, but no specificity or potency data are provided in this listing. Do not assume target engagement without supporting documentation.

Domande frequenti

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 689270-18-6, the molecular formula is C27H33N3O2, and the molecular weight is 431.57 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

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