Kindlin 2 Mouse mAb - Anticorpo primario, specific to FERMT2

Cat. No.: Ab112146

GRADE & PURITY Validated, KO Validation, ExactAb™, See COA

Application

IF/ICC, IHC, IP, WB

Host species

Mouse

Reactive species

consiglio, Mouse, Scimmia, Tuttavia, Umano

Isotype

Mouse IgG2a

Clone Number

OTI14A11

Conjugation

Unconjugated

Panoramica

Mouse anti Human Kindlin 2 Antibody, Monoclonal (OTI14A11), could be used for WB, IHC, IF/ICC, IP and so on.

Specifications

Product Name

Kindlin 2 Mouse mAb - Anticorpo primario, specific to FERMT2

Sinonimi

FLJ34213 | FLJ44462 | UNC112 | FERM2_HUMAN | Fermitin family homolog 2 | Fermt2 | KIND2 | Kindlin-2 | MIG-2 | MIG2 | Mitogen-inducible gene 2 protein | PH domain-containing family C member 1 | Pleckstrin homology domain containing family C (with FERM doma

Specifiche e purezza

KO Validation, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

Specie ospite

Mouse

Specificità

FERMT2

Immunogeno

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Controllo positivo

WB: Hep G2, HeLa, A549, NIH/3T3 and C6 cell lysates. IHC: Human pancreas tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY FERMT2, Hep G2 cells. IP: HEK293T lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, KO Validation, Validated

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 100.0 s

Western Blot: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 30.0 s

KO: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - KO
Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT) and FERMT2-Knockout HeLa cells (KO) were separated by SDSPAGE and immunoblotted with Kindlin 2 Mouse mAb (Ab112146) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

IF/ICC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Kindlin 2 Mouse mAb (Ab112146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2.

IF/ICC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Immunofluorescent staining of Hep G2 cells using Kindlin 2 Mouse mAb (Ab112146).

IHC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Kindlin 2 Mouse mAb (Ab112146). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min).

Applicazione

• Applicazione: Dilution info
• WB:

  1/1000-1/2000 ;

• IF/ICC:

  1/50-1/100

• IHC:

  1/50

• IP:

  2ug/500ul ;

Obiettivi associati (umani)

FERMT2 Tbio Fermitin family homolog 2 (0 Activities)

Discover Target: FERMT2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does KO Validation mean?

KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Kindlin 2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.