GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.for Fluorescence analysis?Fluorescence-analysis grade — very low fluorescent impurities for clean spectra. Use in fluorescence assays where background interferes.for Microscopy?Microscopy grade — reagents/stains suited to sample prep and imaging. Use in microscopy where clarity and low background are needed.≥98%(HPLC)
BioReagent, for Fluorescence analysis, for Microscopy, ≥98%(HPLC) BioReagent,per l'analisi a fluorescenza,per la microscopia for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Desiccated Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
L-ANAP (ANAP) is a bright fluorescent amino acid that can be site-specifically incorporated into proteins using amber stop codon suppression.
Application
L-ANAP may be used to fluorescently label proteins and study its localization and dynamics within live mammalian cells.
Biochem/physiol Actions
L-ANAP (ANAP), a fluorescent structural probe, enables real-time monitoring of ion channel dynamics through site-specific incorporation and environmental sensitivity. ANAP features optimal spectral properties with λex ~ 360 nm and environment-dependent λem ~ 490 nm, Φ of 0.22–0.5, and an ϵ of 17.5 mM⁻¹ cm⁻¹ in water. Due to its environmental sensitivity and ability to serve as a FRET donor, ANAP enables simultaneous monitoring of protein conformational changes, ligand binding, protein-protein interactions, and temperature sensitivity while maintaining channel function. ANAP’s versatility is demonstrated through successful applications in various cell types (Xenopus oocytes, HEK-293, CHO, tSA-201 cells), where it can be combined with electrical recordings to provide direct correlation between structural states and channel function. ANAP’s small size, environment-sensitive fluorescence, and the capability to introduce it at specific sites in the proteome through simple mutagenesis make it a unique and valuable tool for eukaryotic cell biology.
Specifications
Specifiche e purezza
BioReagent, for Fluorescence analysis, for Microscopy, ≥98%(HPLC)
Stabilità e conservazione
Store at 2-8℃ long term (12 months). Store in the dark. Desiccated.
Condizioni di conservazione di stoccaggio
Store at 2-8°C,Protected from light,Desiccated
Spedito in
Wet ice
Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.
Grado
BioReagent, per l'analisi a fluorescenza, per la microscopia
Purezza
≥98%(HPLC)
Nomi e identificatori
CAS alternativo
1313516-26-5 (free base)
Peso molecolare
386.32
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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