Lamin A/C Mouse mAb - Anticorpo primario, specific to Lamin A

Cat. No.: Ab210151

GRADE & PURITY Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

consiglio, Umano

Isotype

Mouse IgG2b

Clone Number

AB07/4G6

Conjugation

Unconjugated

Panoramica

Mouse anti Human Lamin A/C Antibody, clone AB07/4G6, could be used for WB, Flow cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

Lamin A/C Mouse mAb - Anticorpo primario, specific to Lamin A

Sinonimi

70 kDa lamin antibody | Cardiomyopathy dilated 1A (autosomal dominant) antibody | CDCD1 antibody | CDDC antibody | CMD1A antibody | CMT2B1 antibody | EMD2 antibody | FPL antibody | FPLD antibody | FPLD2 antibody | HGPS antibody | IDC antibody | Lamin A an

Specifiche e purezza

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Meccanismi biochimici e fisiologici

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal

Specie ospite

Mouse

Specificità

Lamin A

Immunogeno

Recombinant protein corresponding to Human Lamin A

Controllo positivo

WB: HeLa, A549, MCF7, THP-1, K562 and C6 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells

Coniugazione

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150.0 KDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Immagini

Western Blot: Lamin A/C Mouse mAb (Ab210151)

Lamin A/C Mouse mAb (Ab210151) - Western Blot
All lanes: Lamin A/C Mouse Antibody (Ab210151) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 74, 65 kDa
Observed band size: 72, 65 kDa
Exposure time: 10.0 s

Flow Cytometry: Lamin A/C Mouse mAb (Ab210151)

Lamin A/C Mouse mAb (Ab210151) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Lamin A/C Mouse mAb (Ab210151) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

ICC/IF: Lamin A/C Mouse mAb (Ab210151)

Lamin A/C Mouse mAb (Ab210151) - ICC/IF
IF analysis of Lamin A/C (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (Ab210151) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Applicazione

Applicazione

Dilution info

IF/ICC

1/200

WB

1/20000 – 1/200000

Flow Cytometry

1/800 – 1/50000

Obiettivi associati (umani)

LMNA Tbio Prelamin-A/C (0 Activities)

Discover Target: LMNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Lamin A/C Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.