LAT2 Mouse mAb - Anticorpo primario, specific to LAT2, Mouse IgG1

Cat. No.: Ab112832

QUALITÀ & PUREZZA Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

K52022_7A12

Conjugation

Unconjugated

Panoramica

Mouse anti Human LAT2 Antibody, Monoclonal (K52022_7A12), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/100-1/200
Protein Function
Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
Post-translational
Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
May be polyubiquitinated.

Specifiche

Product Name

LAT2 Mouse mAb - Anticorpo primario, specific to LAT2, Mouse IgG1

Sinonimi

LAB antibody | LAT2 antibody | Linker for activation of B cells antibody | Linker for activation of B-cells antibody | Linker for activation of T cells family member 2 antibody | Linker for activation of T cells transmembrane adaptor 2 antibody | Linker f

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Specie ospite

Mouse

Specificità

LAT2

Immunogeno

Recombinant protein of human LAT2 (AA 121-220).

Controllo positivo

WB: U937, Raji and THP-1 cell lysates. IHC: Human tonsil tissue.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -80°C,Avoid repeated freezing and thawing

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: LAT2 Mouse mAb (Ab112832)

LAT2 Mouse mAb (Ab112832) - Western Blot
All lanes: LAT2 Mouse mAb (Ab112832) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 27 kDa
Exposure time: 62.8 s

IHC: LAT2 Mouse mAb (Ab112832)

LAT2 Mouse mAb (Ab112832) - IHC
IHC analysis of tonsil tissue by LAT2 Mouse mAb (Ab112832).IHC was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with LAT2 Mouse mAb (Ab112832) at 7.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm and membrane. 

Western Blot: LAT2 Mouse mAb (Ab112832)

LAT2 Mouse mAb (Ab112832) - Western Blot
All lanes: LAT2 Mouse mAb (Ab112832) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 27 kDa
Exposure time: 100.0 s

Applicazione

Applicazione

Dilution info

IHC

1/100 - 1/200

WB

1/1000

Obiettivi associati (umani)

LAT2 Tbio Linker for activation of T-cells family member 2 (0 Activities)

Discover Target: LAT2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (LAT2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.