LC pH Gradient Buffer B - BioReagent

Cat. No.: L1523265

GRADE & PURITY BioReagent

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

pH gradient buffer is an innovative elution buffer (or mobile phase) that changes pH linearly for separation and purification of mAb antibodies, glycoproteins and PTM modified proteins by ion exchange (IEX) chromatography. In pH gradient buffer-based IEX chromatography, the pH of the initial buffer is kept at a constant level to ensure that the charged proteins bind to oppositely charged ions of the stationary phase. Then, the protein is eluted by linearly changing the pH of the buffer so that the protein has a zero net charge and can elute from the column. Reagent Composition: Each bottle contains 2.5 mg BT Surfactant, 850 mg NaCl, and 6 g sulfonate. Resuspension: Dissolve reagents in 500 mL deionized, double-distilled water. pH Adjustment: At room temperature, use pH meter to ensure the pH error is less than 0.2, and use 2 M NaOH or hydrochloric acid to adjust the pH. Elution Buffer (mAb): pH Buffer A, pH 5.5 Elution Buffer B (mAb): pH Buffer B, pH 10.2 Linear pH Range: Linear correlation coefficient > 0.99 at pH 5.5 - 10.2 in columns of IEX chromatography. Reference Procedure of mAb subunit and intact protein: Column: SCX, 10 μm, 2.1 * 250 mm or 2.1 * 50 mm Flow rate: 0.3 mL/min Eluent A: 1×mAb pH buffer A (pH 5.5) Eluent B: 1× mAb pH Buffer B (pH 10.2) Gradient: 0 - 1 min, 0% B; 1 - 31 min, 0 - 100% B; 31 - 34 min, 100% B; 34 - 40 min, 0% B Temperature: 30 °C FLR detection: Ex: 280 nm, Em: 360 nm UV detection: 280 nm Load: 0.2 - 10 μg Inject volume: 4 μL

Specifications

Specifiche e purezza

BioReagent

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent

Domande frequenti

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.