LY 88329 , CAS No.78693-86-4

CAS: 78693-86-4 Cat. No.: L1444423 Formula: C₂₅H₂₉NO₆ Peso molecolare: 439.50

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

LY 88329 is a potent narcotic antagonist.

Specifications

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nomi e identificatori

Peso molecolare

439.50

Application Protocols

• No validated application protocols are provided for this product in the current listing.

General setup for small-molecule assays (informational):

• Stock preparation: Dissolve in DMSO to 10–50 mM. Vortex/sonicate briefly if needed; filter (0.22 µm PTFE) for analytical use.
• Serial dilution: Use DMSO or assay buffer to prepare a dilution series; maintain consistent final DMSO content across wells/samples.
• Controls: Include vehicle-only controls and, when available, a literature-cited positive control compound.
• Data integrity: Record lot number, preparation date, and storage conditions. Verify concentration by UV or quantitative NMR if the extinction coefficient is known (not provided here).

For any target-specific bioassay, consult primary literature and adapt parameters accordingly.

Biological Roles

• Item-specific biological function or target engagement for LY 88329 is not provided in the Product Data.

General notes:

• Many LY-series compounds are designed as receptor ligands or enzyme modulators; however, without explicit target information for LY 88329 (CAS 78693-86-4), no biological role can be reliably stated.
• Prior to biological experimentation, consult primary literature indexed by the CAS number to determine reported targets, binding constants, and selectivity profiles. Confirm identity/purity via CoA before interpreting biological results.

Research use limitation:

• This material is supplied strictly for research use only. It is not intended for human or animal therapeutic, diagnostic, or clinical applications.

Buffer Applications

This product is a small-molecule research tool and is not a buffering agent.

Practical notes for assay buffers (general guidance):

• Prepare a concentrated stock in DMSO or ethanol, then dilute into the experimental buffer immediately before use (typical final organic content 0.1–1% v/v).
• Common biological buffers for small-molecule assays include PBS (pH 7.2–7.4), HEPES (pH 6.8–8.2), or Tris (pH 7–9). Select based on target biology and compound stability.
• To mitigate precipitation upon dilution: pre-warm buffer to room temperature, add compound slowly with mixing, and consider minimal surfactant (e.g., 0.01% Tween-80) if assay-compatible.

No item-specific buffer recipes or pH ranges are available; consult literature for guidance once the target/application is identified.

Green Alternatives

Item-specific solvent/reagent hazards are not defined; however, greener practice can still be applied during handling and analysis.

General recommendations (not item-specific):

• Solvent footprint: Prefer acetonitrile or ethanol over halogenated solvents where chromatographic performance allows. For extractions, consider ethyl acetate or 2-MeTHF as replacements for dichloromethane/THF, balancing analyte stability and solubility.
• Energy use: Store at the recommended −20 °C but avoid unnecessary deep-cold storage if stability permits (confirm with CoA). Batch analytical runs to reduce instrument idle time.
• Waste minimization: Prepare small aliquots to reduce repeated freeze–thaw and disposal of expired material. Use micro-scale assays to minimize solvent and consumables.

Illustrative comparison (general):

• Halogenated solvent (e.g., DCM): high EHS burden; often replace with ethyl acetate or 2-MeTHF when compatible.
• DMF/DMSO: excellent solvency but high boiling points; consider ethanol/isopropanol for less hazardous alternatives in non-biological workflows if solubility allows.

Always validate greener substitutions for recovery, stability, and assay performance.

Pharmaceutical Uses

• No pharmacopeial status, excipient role, or formulation use is specified for this product.

Context and limitations:

• LY 88329 is provided for research use only and is not approved for therapeutic, diagnostic, or clinical applications. Do not use in humans or animals.

If used in pre-formulation research (general guidance):

• Conduct basic pre-formulation screens (solubility vs pH, cosolvent tolerance, light/oxidation sensitivity) to guide in vitro study design.
• For analytical method development supporting formulation research, establish stability-indicating LC–UV/LC–MS methods and evaluate forced-degradation pathways.

Any implication of clinical utility is outside the scope of this product listing.

Physical Properties

Item-specific physicochemical data are not provided in this record.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point (mp): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (bp): Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• pKa / logP: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General laboratory guidance (literature/practice, not item-specific):

• For uncharacterized bioactive small molecules, initial dissolution screening commonly uses anhydrous DMSO (10–100 mg/mL stock), ethanol (5–50 mg/mL), or DMF. Aqueous buffers are used after making a concentrated organic stock.
• If weakly basic, improved solubility may be achieved in slightly acidic aqueous media (e.g., 0.1% HCl); if weakly acidic, solubility may increase in basic media (e.g., 0.1% NaOH). Validate stability before pH adjustment.
• Determine solubility empirically at small scale; inspect for haze/precipitate after dilution into assay buffers.

Important: Treat any physical constants found in secondary sources as informational until confirmed by a lot-specific CoA.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and implications (general guidance):

• When a purity grade is not listed, Aladdin Scientific provides a lot-specific CoA detailing assay purity (e.g., HPLC area%), and may include orthogonal ID tests (NMR, MS) and residual solvent metrics. Request the CoA for your lot to confirm suitability for regulated workflows.
• For bioactive reference compounds, typical priorities include: high HPLC purity, single major component by MS, and well-resolved chromatographic profile. Low UV background is less critical unless used in analytical calibration.
• If a stabilizer or salt form is used, it will be declared on the label/CoA. None is specified in the current record.

Documentation:

• For method validation, request additional documents (CoA, SDS, TDS). If elemental impurities, water content (KF), or residual solvent limits are critical to your application, specify this at purchase so a matching lot can be allocated.

Reaction and Applications

Manufacturer-specified applications are not listed for this item.

Context (general):

• LY-series designations are commonly used for bioactive small molecules developed as research tools. Without target data, LY 88329 should be treated as a generic reference compound for exploratory biology, receptor/pharmacology screening, or analytical calibration—pending verification from the CoA or literature using the CAS number.

Potential laboratory uses (general guidance; not item-specific claims):

• In vitro assay development: Prepare defined-concentration DMSO stocks for dose–response or mechanism-of-action studies once the biological target is confirmed from literature.
• Analytical reference: Use for retention-time locking, detector response factors, or LC–MS method qualification after confirming identity/purity.
• Stability studies: Subject to accelerated conditions (e.g., 40 °C, light exposure) to characterize degradation pathways; inform storage/handling.

Important:

• Do not infer a pharmacological target from the LY prefix alone. Verify the intended application and target specificity via primary references linked to CAS 78693-86-4 before use in decision-making experiments.

Reaction Conditions

No synthetic or reaction-condition information is provided for this item.

General guidance (analytical/handling rather than synthesis):

• For LC–MS: start with a water/acetonitrile gradient containing 0.1% formic acid (or 5–10 mM ammonium formate for MS-friendly buffering). Adjust pH once ionization characteristics are known.
• For stability studies: evaluate temperature (4, 25, 40 °C), light exposure, and oxidative stress (air vs inert atmosphere) to define handling conditions.

Without structural details, specific reagents, catalysts, or temperature ranges for chemical transformations cannot be recommended.

Safety and Handling

Safety details specific to this item are not provided in the current record.

• GHS Classification: Not specified for this item; refer to SDS.
• Signal Word / Pictograms / H-Statements: Not specified for this item; refer to SDS.

General laboratory precautions (good practice; defer to SDS for authoritative guidance):

• PPE: Lab coat, appropriate chemical-resistant gloves (e.g., nitrile), safety glasses or face shield when splash risk exists. Handle powders in a fume hood.
• Engineering controls: Use a certified chemical fume hood; avoid aerosol generation and inhalation of dust.
• Incompatibilities: Unknown for this specific item; as a precaution, segregate from strong oxidizers and strong acids/bases until compatibility is known.
• Hygiene: Avoid skin/eye contact; wash thoroughly after handling. Do not pipette by mouth.
• First aid (overview): In case of skin contact, wash with soap and water; eye contact, rinse cautiously with water for several minutes; inhalation, move to fresh air; ingestion, rinse mouth. Seek medical attention and provide the SDS.
• Spill response: For small quantities, absorb with inert material and collect as hazardous waste. Prevent dust formation.

Note: Obtain and consult the product-specific SDS prior to first use. This product is supplied strictly for research use only.

Solvent Selection

Item-specific solvent preferences are not provided.

Practical strategy for small-molecule tool compounds (general guidance):

• Primary stock solvent: Start with anhydrous DMSO due to broad solvating power and compatibility with biochemical assays. Prepare 10–50 mM stocks unless solubility dictates otherwise.
• Secondary solvents: Ethanol or DMF for orthogonal dissolution checks. For analytical prep, acetonitrile or methanol are common.
• Assay dilution: Dilute DMSO stocks into buffer just before use; maintain final DMSO ≤0.1–1% v/v to minimize biological interference.
• Solubility verification: After dilution, inspect for precipitation within 10–30 minutes; if present, increase co-solvent fraction or include non-ionic surfactant (e.g., 0.01–0.05% Tween-80) if compatible with the assay.

Selection notes:

• If the compound is hydrophobic (unknown here), consider limited aqueous compatibility and use higher organic content for LC method development (e.g., 60–90% MeCN with 0.1% formic acid).
• If ionizable (pKa unknown), manipulate mobile phase pH to control retention and peak shape.

Conclusion: Choose DMSO for stocks and validate compatibility with your downstream system. Confirm item-specific solubility from the CoA when available.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Store at −20 °C (per Product Data).
• Shipped In: Ice chest + ice pads (per Product Data).

Practical guidance (general best practices):

• Aliquoting: Upon receipt, equilibrate to room temperature before opening to prevent condensation. Prepare small, single-use aliquots to minimize freeze–thaw cycles.
• Container: Use amber vials if the compound is light-sensitive (sensitivity not specified). Purge headspace with inert gas (N2 or Ar) if oxidation is a concern.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. As a general starting point, dissolve in anhydrous DMSO to prepare a concentrated stock, then dilute into working buffer immediately before use.
• Stability: Not specified for this item; refer to CoA/Spec Sheet. Monitor for discoloration or precipitation; discard if degradation is suspected.

Research use note:

• For research use only. Not for human or animal use.

Structure and Identity

Brief overview: This listing refers to LY 88329 (CAS 78693-86-4). Detailed structural identifiers are not provided in the current product record.

• SKU: L1444423
• Product Name: LY 88329
• CAS: 78693-86-4
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• Synonyms: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general guidance):

• Item-specific functional groups, ring systems, and stereochemistry are not provided in the Product Data. Please consult the CoA/SDS or primary literature indexed by the CAS number for definitive structural details.

2D structure description:

• Not available in the current record. If you require a structure diagram for method development or regulatory documentation, please request the CoA/Spec Sheet or retrieve from a structure database using the CAS number.

Notes on identity confirmation (general):

• For small-molecule research tools like LY-series compounds, identity is typically verified by a combination of NMR, LC–MS/HRMS, and HPLC purity. Request the lot-specific CoA for analytical details.

Synthetic Utility

• This item is supplied as a bioactive reference/tool compound rather than as a synthetic building block. No reactive handles or transformation guidance are provided in the Product Data.

General considerations (if using as a reference standard or analyte):

• Develop a robust analytical route (e.g., LC–MS with graded aqueous/organic mobile phase) for purity confirmation and stability tracking.
• If derivatization is desired for analytical purposes (e.g., to improve detection), choose conditions that are minimally intrusive and reversible; however, without a known structure, no derivatization scheme can be recommended.

If synthetic modification is required, obtain full structural information (CoA/SDS/literature) before planning transformations.

Target Specificity

• No target, selectivity profile, or binding data are provided for LY 88329 in the Product Data.

Guidance:

• If target engagement is critical, consult peer-reviewed literature using CAS 78693-86-4 to source Ki/IC50/EC50 values, off-target panels, and species cross-reactivity. Confirm the salt form and stereochemistry (if applicable) to ensure data comparability.