Lysozyme Solution (10 mg/mL) - BioReagent,Suitable for molecular biology,10 mg/mL

Cat. No.: L1520263

GRADE & PURITY Suitable for molecular biology, BioReagent, 10 mg/mL

Synonyms

Muramidase Solution

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

DNA Extraction

Panoramica

Lysozyme, also known as muramidase, is an alkaline enzyme capable of hydrolyzing the mucopolysaccharides present in pathogenic bacteria. Its mechanism of action involves breaking the β-1,4 glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine in the bacterial cell wall. This breaks down the insoluble mucopolysaccharides in the cell wall into soluble glycopeptides, leading to cell wall rupture, leakage of cellular contents, and ultimately, bacterial lysis.

The lysozyme solution (10 mg/mL) is composed of lysozyme and TRIS among other components, with a pH of approximately 8.0. It is primarily used to lyse bacterial cell walls to facilitate subsequent plasmid DNA extraction procedures.

This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other uses.

Product Features

• High heat resistance, maintaining activity within a certain temperature range.
• Insensitive to organic solvents, which cannot completely inactivate it.
• Stable activity, ensuring long-term effective enzymatic effects.
• Exhibits antibacterial properties with a broad antibacterial spectrum.
• Possesses effective preservative qualities, aiding in the protection of experimental samples.

Operating Procedure (For reference only)

Follow specific experimental requirements. Generally, add this reagent at a volume ratio of bacterial suspension to lysozyme solution ranging from 6:1 to 10:1.

Precautions

• If using small volumes each time, it is advisable to aliquot the reagent appropriately to avoid repeated freeze-thaw cycles that may affect activity.
• To ensure the safety and health of experimenters, please wear a lab coat and disposable gloves during operation.
• Use the reagent promptly after opening to prevent a decrease in activity that could affect subsequent experimental results.

Specifications

Sinonimi

Muramidase Solution

Specifiche e purezza

BioReagent,Suitable for molecular biology,10 mg/mL

Stabilità e conservazione

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent, Suitable for molecular biology

Nomi e identificatori

• PH: 8.0

Domande frequenti

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

What does Suitable for molecular biology mean?

Suitable for molecular biology indicates that the product has been processed and tested for molecular biology and proteomics. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.