This product is a special staining reagent for histopathology, mainly used for the specific staining of amyloid substances (such as AL-type and AA-type amyloidosis) in paraffin sections and frozen sections. The distribution and morphology of amyloid substances can be observed under light microscopy, and they exhibit characteristic apple-green birefringence under polarized light, which is used for pathological diagnosis and related experimental research.
Usage (for reference only, taking paraffin sections as an example):
1. Deparaffinization to water: Place the paraffin sections sequentially in Xylene I (5 min) → Xylene II (5 min) → 100% ethanol (2 min) → 95% ethanol (2 min) → 80% ethanol (2 min), and finally rinse with distilled water twice, 1 min each time.
2. Staining: Immerse the sections in 0.5% methanol Congo Red staining solution and stain at room temperature for 10–20 min. Gently agitate the staining solution during the process to ensure uniform staining.
3. Differentiation: Take out the sections and differentiate rapidly in alkaline ethanol differentiation solution (0.2% KOH dissolved in 80% ethanol) for 5–10 seconds. Monitor under a microscope until the background is nearly colorless and the amyloid regions retain red color, then remove immediately.
4. Washing and bluing: Rinse the sections under running water for 3–5 min to completely remove residual differentiation solution and avoid affecting subsequent staining results.
5. Nuclear counterstaining: Counterstain the sections in Mayer's hematoxylin solution for 1–2 min, then rinse under running water until the cell nuclei appear blue.
6. Dehydration and clearing: Place sequentially in graded ethanol (95% ethanol 2 min → 100% ethanol 2 min, twice) → Xylene (5 min, twice) to clear the sections.
7. Mounting and observation: Add neutral balsam onto the sections, cover with a coverslip, and after the balsam dries, observe under light microscopy or polarized light microscopy.
Staining Results:
Light microscopy: Amyloid substances appear orange-red, cell nuclei appear blue, and the background is colorless or pale pink.
Polarized light microscopy: Amyloid substances exhibit characteristic apple-green birefringence, indicating a positive result.
Precautions:
1. For your safety and health, please wear a laboratory coat and disposable gloves during operation.
2. This product is for research use only; strictly prohibited for other purposes.
ZJ26F0434646