MMRi62 - 10mM in DMSO , CAS No.352693-80-2

CAS: 352693-80-2 Cat. No.: M655463 Formula: C₂₁H₁₅Cl₂N₃O Peso molecolare: 396.27

QUALITÀ & PUREZZA 10mM in DMSO

Storage

Protected from light,Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

MMRi62, a ferroptosis inducer targeting MDM2-MDM4 (negative regulators of tumor suppressor p53). MMRi62 shows a P53-independent pro-apoptotic activity against pancreatic ductal adenocarcinoma (PDAC) cells and induce autophagy. MMRi62 induces ferroptosis , resulting in a increase of reactive oxygen and lysosomal degradation of ferritin heavy chain (FTH1). MMRi62 also leads to proteasomal degradation of mutant p53, also inhibits orthotopic xenograft PDAC mouse model in vivo with high frequency mutation characteristics of KRAS and TP53.12.

In Vitro

MMRi62 inhibits proliferation, clonogenic, and spheroid growth of pancreatic ductal adenocarcinoma cell (PDAC) by induction of cell death. 1\nMMRi62 (3 nM-100 μM;4 h) binds to RING–RINGheterodimers of MDM2 and MDM4 withthe K d value of 1.39 μM. MMRi62 (10 nM-1 μM;72 h) induces apoptosis and inhibits leukemic cells with IC 50 sof 0.34 µM (HL60) and 0.22 µM (HL60VR). MMRi62 (5 μM and 10μM; 24 h) decreases MDM2B autoubiquitination, increasesMDM4 ubiquitination in a dose-dependent manner. MMRi62 is an E3 ligase modifier capable ofswitching substrate preference from MDM2 to MDM4. MMRi62 (5 μM; 24and 72 h) induces apoptosis in a p53-independent manner. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: WT-p53 bearing MV4-11 cells; 293cells transfected with MDM2B and MDM4 Concentration: 2, 2.5, 5, 10, 40, 80, 160 μM Incubation Time: 24 hours Result: Increased cleaved PARP protein and activatedcaspase 3 level in wt-p53 bearing MV4-11 cells at 2 μM for 24 h. Decreased MDM2B autoubiquitination, increasedMDM4 ubiquitination at 5 μM and 10 μM for 24 h. Induced MDM2-dependent degradation of MDM4protein at 5 μM in NALM6 cells. Cell Proliferation AssayCell Line: Primary AML patient cells, NALM6cells and NALM6shp53 cells Concentration: 1, 10, 25, and 50 µM Incubation Time: 24 hours and 72 hours Result: Induced NALM6 cells apoptosis at 24 hand induced Primary AML patient cells at 72 h.

In Vivo

MMRi62 shows anti-tumor activity in orthotopic xenograft PDAC mouse models, by inhibiting tumor growth in mice associated with downregulation of NCOA4 and mutant p53 . MMRi62 also completely abrogates metastasis of orthotopic tumors . MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Specifiche

Specifiche e purezza

10mM in DMSO

Meccanismi biochimici e fisiologici

MMRi62, a ferroptosis inducer targeting MDM2-MDM4 (negative regulators of tumor suppressor p53). MMRi62 shows a P53-independent pro-apoptotic activity against pancreatic ductal adenocarcinoma (PDAC) cells and induce autophagy . MMRi62 induces ferroptosis

Condizioni di conservazione di stoccaggio

Protected from light,Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nomi e identificatori

Peso molecolare

396.27

Domande frequenti

How should this product be stored?

Store at ?80 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 352693-80-2, the molecular formula is C21H15Cl2N3O, and the molecular weight is 396.27 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.