Mouse 4-Hydroxynonenal (4-HNE) ELISA Kit
Disponibile
SKU
EJ1515455
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse 4-HNE antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse 4-HNE in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
Maggiori Informazioni
| Specifiche e purezza | BioReagent |
|---|---|
| Campo di rilevamento | 0.625ng/mL~40ng/mL |
| Specie rilevata Oggetto | Mouse |
| Specificità | Detecting Mouse 4-HNE in samples, with no significant cross-reactivity to other homologs. |
| Product Description | This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse 4-HNE antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse 4-HNE in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve. |
| Condizioni di conservazione di stoccaggio | Store at 2-8°C |
| Spedito in | Wet ice |
| Stabilità e conservazione | Store at 2-8℃ for 6 months. |
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