Mouse IgG1-kappa, anti-E8L - Isotype Control Antibody, specific to E8L

Cat. No.: Ab213557
AVAILABLE TO ORDER
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
ELISA, Flow Cytometry
Host species
Mouse
Reactive species
Virus
Isotype
Mouse IgG1
Clone Number
EF12/6D11
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab213557-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$27.90
1mg
Ab213557-1mg
1
$109.90
5mg
Ab213557-5mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$447.90
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse IgG1, Isotype Control Antibody (EF12/6D11), could be used for Flow Cytometry analysis, ELISA and so on.

Specifications

Product Name
Mouse IgG1-kappa, anti-E8L - Isotype Control Antibody, specific to E8L
Synonyms
Mouse IgG1, κ Isotype Control | Purified Mouse IgG1, kappa Isotype Control | Mouse IgG1 Isotype Control Antibody | Mouse IgG1 Isotype Control mAb | Immunoglobulin G1 Fc | Mouse IgG1 Isotype Control | Isotype control mAb for mouse IgG1 | IgG1 (Mouse) Isoty
Specifications & Purity
Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chai
Host species
Mouse
Specificity
E8L
Immunogen
Recombinant protein corresponding to MPXV E8L
Positive Control
Flow Cytometry: Peripheral blood mononuclear cells; Mouse splenocytes
Conjugation
Unconjugated
Grade
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

Mouse IgG1-kappa, anti-E8L (Ab213557) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood mononuclear cells labelling CD40 (Red) with CD40 Mouse mAb (Ab094768) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, compared to concentration matched Mouse IgG1-kappa, anti-E8L (Ab213557) (Blue) conjugated with biotin. Detected with SA-PE (rp156150).

Mouse IgG1-kappa, anti-E8L (Ab213557) - ICC/IF
IF analysis of HeLa cells using beta Tubulin Mouse mAb (Ab179922) (Green) compared to concentration matched Mouse IgG1-kappa, anti-E8L (Ab213557). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. A Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used at a dilution of 1/1000. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application
ApplicationDilution info
Flow Cytometry

Use at the same concentration as the detection antibody.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Animal Free grade guide → View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

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