MSI2 Mouse mAb - Anticorpo primario, specific to MSI2, Mouse IgG1

Cat. No.: Ab116122

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

ELISA, IHC, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

2C11

Conjugation

Unconjugated

Panoramica

Mouse anti Human MSI2 Antibody, Monoclonal antibody, could be used for WB, IHC, ELISA and so on.

Application:
WB: 1/500 - 1/2000. Predicted molecular weight: 35 kDa.
IHC: 1/200-1/1000
ELISA: 1/10000

Protein Function:
Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to posttranslational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.Tissue specificity: Ubiquitous; detected at low levels.
RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

Post-translational:
Phosphorylated.

Specifications

Product Name

MSI2 Mouse mAb - Anticorpo primario, specific to MSI2, Mouse IgG1

Sinonimi

FLJ36569 antibody | MGC3245 antibody | Msi2 antibody | MSI2/HOXA9 fusion gene, included antibody | MSI2H antibody | MSI2H_HUMAN antibody | Musashi 2 antibody | Musashi homolog 2 antibody | Musashi RNA binding protein 2 antibody | Musashi, Drosophila, homo

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Specie ospite

Mouse

Specificità

MSI2

Immunogeno

Purified recombinant fragment of human MSI2(AA: 1-190) expressed in E. Coli.

Controllo positivo

WB: HeLa, Hep G2 and A549 cell. IHC: Ovarian cancer tissues, Cerebellum tissues.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

1.0mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Immagini

Western Blot: MSI2 Mouse mAb (Ab116122)

MSI2 Mouse mAb (Ab116122) - Western Blot
All lanes: MSI2 Mouse mAb (Ab116122) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35, 37 kDa
Exposure time: 126.8 s

Western Blot: MSI2 Mouse mAb (Ab116122)

MSI2 Mouse mAb (Ab116122) - Western Blot
All lanes: MSI2 Mouse mAb (Ab116122) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35, 39 kDa
Exposure time: 37.0 s

IHC: MSI2 Mouse mAb (Ab116122)

MSI2 Mouse mAb (Ab116122) - IHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer using MSI2 Mouse mAb (Ab116122) with DAB staining.

IHC: MSI2 Mouse mAb (Ab116122)

MSI2 Mouse mAb (Ab116122) - IHC
Immunohistochemical analysis of cerebellum tissues using MSI2 Mouse mAb (Ab116122) with DAB staining.

Applicazione

• Applicazione: Dilution info
• ELISA:

  1/10000 ;

• IHC:

  1/200-1/1000 ;

Obiettivi associati (umani)

MSI2 Tbio RNA-binding protein Musashi homolog 2 (0 Activities)

Discover Target: MSI2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MSI2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.