Mycoplasma Broth Medium (ChP) - BioReagent

Cat. No.: M1528684

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Panoramica

Product application: For the cultivation of Mycoplasma. Usage: Weigh 27.5 g of this product in 1 L of purified water, heat to boiling until completely dissolved, dispense, autoclave at 121°C for 15 minutes, and set aside for use. Formula: g/L Porcine stomach digest 10.0 g Beef extract powder 5.0 g Yeast extract powder 5.0 g Sodium chloride 2.5 g Glucose 5.0 g Phenol red 0.02 g pH 7.6 ± 0.2 (25°C)

Specifications

Specifiche e purezza

BioReagent

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Grado

BioReagent

Product Line

Prodotti biologici

Application Protocols

No vendor-validated protocols are provided in the Product Data. Below is general, non-item-specific guidance for research use. Always adjust based on your method and consult the CoA/Spec Sheet.

General enrichment workflow (literature/general)

1. Preparation: Reconstitute the dehydrated medium in purified water at the labeled concentration. Sterilize by autoclaving if compatible, or sterile-filter. Cool to 45–50 °C before adding heat-labile supplements.
2. Supplementation: Add required sterile supplements (e.g., serum/sterols, selective antibiotics) if your method specifies them. Mix gently to avoid foaming.
3. Inoculation: Introduce test sample (e.g., cell culture supernatant, raw material extract) under aseptic conditions. Include positive and negative controls.
4. Incubation: Culture at the specified temperature/time for your method (often multiple days). Agitation may be gentle or static per protocol.
5. Monitoring: Check for turbidity or indicator color change if present. At defined intervals, subculture to mycoplasma agar or perform PCR/qPCR to confirm detection.
6. Documentation: Record lot numbers, preparation details, incubation parameters, and control results.

Note

• Exact preparation grams per liter, pH targets, and supplement identities are Not specified for this item; refer to CoA/Spec Sheet.
• For research use only (per Product Data).

Biological Roles

Mycoplasma Broth Medium (ChP) is designed to support the biological requirements of Mycoplasma spp., which are fastidious, sterol-requiring, wall-less bacteria. While the medium itself has no intrinsic biological role, it supplies nutrients and physicochemical conditions that enable growth and metabolic activity of these organisms (general/literature context).

Key biological considerations (general)

• Sterol dependence: Many mycoplasmas require cholesterol/sterols for membrane integrity; broths may be supplemented with serum or defined sterol solutions.
• Minimal biosynthetic capacity: Reduced genomes lead to extensive nutrient auxotrophies (amino acids, nucleotides, fatty acids), necessitating rich, complex media.
• pH and redox: Mycoplasma growth can shift medium pH; inclusion of buffers and indicators (e.g., phenol red) facilitates monitoring (when present).
• Osmotic sensitivity: Wall-less cells are sensitive to osmotic stress; isotonic formulations support viability during enrichment.

Use contexts (research)

• Contaminant detection/enrichment from mammalian cell cultures prior to analysis.
• Isolation and propagation of reference strains for assay development and basic microbiological studies.

Item-specific composition that determines exact biological performance is Not specified for this item; refer to CoA/Spec Sheet. Always verify growth-promotion with relevant strains when adapting the medium to a new workflow. For research use only (per Product Data).

Buffer Applications

This product is a complex culture medium, not a defined buffering reagent. It is not typically used to make analytical buffers or electrophoresis running solutions.

• If buffering capacity is required, the medium’s built-in buffer system (if any) should be considered part of the growth formulation and not altered without validation (general guidance).
• For analytical buffer preparation, choose dedicated buffer components (e.g., phosphate, HEPES, MOPS) rather than microbiological media.

For exact prepared pH targets or buffering components present in this product, details are Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Sustainability considerations for culture media focus on resource use, waste, and animal-derived components rather than solvent replacement.

Opportunities (general)

• Dehydrated format: Shipping a dry powder reduces mass and refrigeration needs compared with ready-to-use liquid media, lowering transport emissions.
• Right-sizing batches: Prepare only the volume required to minimize expired waste; reconstituted broths have limited shelf life.
• Energy-aware sterilization: Use validated cycle loads and avoid excessive autoclave time; consider sterile filtration for heat-labile supplements to reduce repeated thermal cycles.
• Animal-origin reduction: Where scientifically appropriate, evaluate serum-free or chemically defined supplements to reduce reliance on animal-derived materials (note: applicability depends on method requirements; many mycoplasma require sterols/serum—literature/general).
• Waste management: Deactivate biological waste efficiently (e.g., consolidated autoclave runs) and segregate packaging for recycling where permitted.

Comparison (general)

• Dehydrated broth vs. ready-to-use liquid: lower shipping energy and longer shelf life for dehydrated powder; liquid is more convenient but heavier and often requires cold chain.
• Serum-containing vs. serum-free supplements: serum supports robust growth for many mycoplasma species; serum-free defined systems may reduce variability and animal use but can compromise recovery of unknown strains.

Note

• Product-specific green attributes (e.g., certified animal-free, renewable content) are Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

No therapeutic/clinical claims are made. In a research and manufacturing quality context, mycoplasma broth media are commonly employed in laboratory workflows related to bioprocess support and method development (general information).

Potential roles in regulated environments (general)

• Method development/verification: Used to develop and verify compendial or in-house mycoplasma detection assays (e.g., enrichment steps preceding agar subculture and/or nucleic-acid-based methods).
• Environmental or raw-material screening: Research-stage assessments of bioprocess streams and raw materials for mycoplasma presence.
• Reference/culture maintenance: Propagation of reference strains for positive controls in assay qualification.

Standards and documentation

• The “ChP” designation signals alignment with Chinese Pharmacopoeia guidance for mycoplasma test media. However, item-specific conformity statements, performance criteria, and release tests are Not specified for this item; refer to CoA/Spec Sheet.

Notes

• Any use in GMP-regulated testing requires user qualification of the specific lot, documented growth-promotion testing with relevant strains, and adherence to internal quality procedures. This product is for research use only per Product Data.

Physical Properties

As a dehydrated culture medium mixture, physical constants are not defined by a single value.

Item-specific properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet.
• Osmolality (prepared medium): Not specified for this item; refer to CoA/Spec Sheet.
• Solubility in water: Readily soluble (general for dehydrated media); exact dissolution characteristics for this item not specified.
• BP/MP/Density/RI/LogP: Not applicable to a heterogeneous solid mixture.

General/literature characteristics for mycoplasma broths (for context only)

• Form: Free-flowing dehydrated powder or granules that dissolve in purified water to form a clear to slightly opalescent broth (literature/general).
• Typical preparation: Dissolution in water at the labeled grams per liter, with sterile filtration or autoclaving as appropriate for supplement stability (literature/general).
• Supplementation: Some mycoplasma broths require addition of sterols (e.g., serum or cholesterol), selective antibiotics, and phenol red as an indicator (literature/general). This product’s specific supplement requirements are not specified here.

Important

• Always consult the lot-specific CoA/Spec Sheet for the target prepared pH at 25 °C, clarity, and performance acceptance (e.g., growth promotion with compendial strains), as these are the authoritative specifications for this item.

Quality and Grades

• Item grade: BioReagent (Product Data)

  • Indicates suitability for laboratory research applications. This is not a clinical/diagnostic product. Performance characteristics are intended for research workflows; consult the CoA for acceptance criteria.

• Standard: ChP

  • The “ChP” designation indicates conformance to requirements aligned with the Chinese Pharmacopoeia for mycoplasma testing media preparation and performance (general note). In practice, this often involves growth-promotion suitability with designated mycoplasma strains and inhibitory performance for non-target organisms (literature/general). The exact acceptance tests for this item are Not specified for this item; refer to CoA/Spec Sheet.

• What to expect in documentation

  • Lot-specific CoA typically includes: prepared pH range, clarity, and growth promotion results with compendial strains. For this catalog item, values are Not specified for this item; refer to CoA/Spec Sheet.
  • SDS covers safety, handling, and regulatory statements.

• Stabilizers/additives

  • If any stabilizers or selective supplements are included in the dehydrated base, they will be listed on the CoA/label. Not specified for this item; refer to CoA/Spec Sheet.

• Practical quality notes

  • Use high-purity water and aseptic technique to preserve medium performance.
  • Avoid moisture ingress; caking can compromise homogeneity and performance.
  • Validate performance in your specific assay (e.g., enrichment before PCR/qPCR or culture-based detection) with internal positive controls.

Reaction and Applications

Chemical reaction guidance is not directly applicable to a microbiological medium. Instead, consider application domains for Mycoplasma Broth Medium (ChP) in research and assay workflows.

Research applications (general)

• Enrichment and cultivation of fastidious Mycoplasma spp. from cell culture contaminants, raw materials, or environmental samples prior to downstream analysis (culture, PCR/qPCR, NGS) (literature/general).
• Method development/validation for mycoplasma detection per pharmacopeial chapters (e.g., enrichment steps before agar subculture and indicator systems). The specific compendial chapter alignment for this item is not specified here.
• Antimicrobial evaluation: assessing inhibitory effects of compounds on mycoplasma growth in broth microdilution formats (research use only).

Practical tips

• Use aseptic technique to avoid overgrowth by adventitious bacteria/fungi; supplement with selective agents only if validated for your method.
• Include positive controls (reference mycoplasma strains) and negative controls (uninoculated medium) to verify performance.
• For detection sensitivity, perform multi-day enrichment with periodic subculture or molecular sampling; mycoplasma can have slow growth and require sterols (literature/general).
• Monitor pH indicators (if present) or turbidity changes; confirm presumptive positives using orthogonal assays (e.g., DAPI staining, species-specific PCR) in research settings.

Note

• No medical or diagnostic claims are made. This medium is for research use only, per Product Data.

Reaction Conditions

Not applicable in the sense of chemical reactions. For microbiological use, the closest analogue to “reaction conditions” is the preparation and incubation parameters for culture.

General cultivation guidance (literature/general; not item-specific)

• Reconstitution: Dissolve the labeled mass of dehydrated medium per liter of purified water with mixing. Heat gently if needed to facilitate dissolution. Exact mass and instructions are Not specified for this item; refer to CoA/Spec Sheet.
• Sterilization: Autoclave at validated conditions (e.g., 121 °C for an appropriate time) for the base medium if compatible. Alternatively, sterile-filter the prepared broth when heat-labile components are present.
• Supplements: Add sterile-filtered supplements (e.g., serum, sterols, selective antibiotics) after cooling the base medium to 45–50 °C, if required by your method.
• Incubation: Grow inoculated cultures at the temperature and atmospheric conditions suitable for the target mycoplasma species (often 35–37 °C, CO2 usage per method). Exact conditions vary by species and are not specified for this item.
• Monitoring: Observe turbidity/pH indicator changes where applicable; subculture or perform molecular detection at defined intervals to confirm presence/absence.

Always verify conditions with method references and perform internal growth-promotion controls using relevant strains. For research use only.

Safety and Handling

Hazard information provided with this listing is incomplete; treat the product according to standard microbiological media safety practices and defer to the SDS for authoritative guidance.

GHS/Classification

• Signal word, H-statements, pictograms: Not specified for this item; consult the SDS.

Primary hazards (general for dehydrated microbiological media)

• Low acute toxicity expected; dust may cause mechanical eye/respiratory irritation (literature/general).
• Some formulations may contain animal-derived materials or antibiotics; handle with care to avoid sensitization or environmental release.

PPE and engineering controls

• Wear lab coat, safety glasses, and appropriate gloves (e.g., nitrile). Avoid inhaling dust; handle powders in a fume hood or with local exhaust if dusting occurs.
• Use aseptic technique to avoid contamination and potential exposure to cultured mycoplasma.

First aid (general)

• Inhalation: Move to fresh air; seek medical advice if symptoms persist.
• Skin/eyes: Rinse with water for several minutes; remove contaminated clothing; obtain medical attention if irritation persists.
• Ingestion: Rinse mouth; seek medical advice if unwell.

Storage incompatibilities and precautions

• Store tightly closed in a dry place at room temperature (per Product Data), protected from humidity and microbial contamination.
• Avoid contact with strong oxidizers; prevent moisture uptake which can degrade performance.

Biological safety note

• After inoculation, broth cultures may contain viable mycoplasma. Treat all cultures and waste as biohazardous; autoclave or disinfect before disposal according to institutional biosafety rules.

Solvent Selection

This product is a dehydrated culture medium intended to be reconstituted with water; classical “solvent selection” is not applicable as for organic reagents.

• Recommended diluent (general)

  • Purified water meeting laboratory or pharmacopeial specifications (e.g., water for injection or purified water) is typically used to prepare microbiological broths. The exact water grade required for this item is Not specified for this item; refer to CoA/Spec Sheet.

• Miscibility and polarity

  • The medium components are formulated for complete dissolution/dispersal in aqueous systems. Organic cosolvents are generally not recommended as they can inhibit mycoplasma growth (literature/general).

• Practical guidance

  • Add the labeled mass of dehydrated medium to an appropriate volume of water with mixing. Heat gently if needed to aid dissolution, avoiding boiling that can cause Maillard reactions or caramelization.
  • If heat-labile supplements are required (e.g., serum, antibiotics, sterols), sterilize the base by autoclaving and then add sterile-filtered supplements after cooling to 45–50 °C (general practice).

• Comparison to alternatives

  • Broth vs. agar: Use broth for enrichment and high-sensitivity detection; agar media enable colony isolation and morphology assessment (literature/general).
  • Ready-to-use liquids can save time but have shorter shelf life; dehydrated powder offers longer storage and flexible batch sizing.

Storage and Reconstitution

Storage

• Storage conditions: Room temperature (per Product Data). Keep container tightly closed, dry, and protected from humidity and light. Avoid temperature extremes and repeated opening that may introduce moisture.
• Shipping: Normal (per Product Data). Inspect on receipt; if caked or humidified, performance may be affected.

Reconstitution (general guidance; not item-specific)

• Water quality: Use purified water meeting your laboratory or pharmacopeial requirements. Degas if excessive foaming occurs.
• Dissolution: Add the labeled mass of dehydrated medium to water with continuous stirring until fully dissolved. Gentle heating can assist dissolution; do not boil.
• Sterilization: Autoclave the base medium if heat-stable. If the formulation includes heat-labile components, prepare by sterile filtration or add those components aseptically after autoclaving and cooling to 45–50 °C.
• Supplements: Add sterile-filtered supplements (e.g., serum/sterols, antibiotics) only if required by your method. The need for and identity of supplements for this item are Not specified for this item; refer to CoA/Spec Sheet.

Prepared medium handling (general)

• Store prepared broth at 2–8 °C, protected from light if indicators are present; use within the validated shelf life for your laboratory. Exact shelf life is Not specified for this item; refer to CoA/Spec Sheet.

Research Use Note

• For research use only (per Product Data). Always consult the CoA and SDS for authoritative, lot-specific instructions.

Structure and Identity

Mycoplasma Broth Medium (ChP) is a complex, multi‑component microbiological growth medium formulated for the enrichment and cultivation of Mycoplasma species. As a mixture, it does not possess a single defined molecular structure.

• Item identity
  • Product name: Mycoplasma Broth Medium (ChP) (SKU: M1528684)
  • Grade/Purity: BioReagent (per Product Data)
  • Category: Life Science – Culture Media
  • Standard: ChP indicates alignment with Chinese Pharmacopoeia guidance for mycoplasma test media (general note; consult method/monograph for details).
• Composition nature
  • Complex mixture typically comprising peptones/tryptones, yeast-derived nutrients, buffering salts, and selective supplements (literature/general). Exact composition for this catalog item is not specified here.
• Structural identifiers
  • Molecular formula: Not applicable to mixtures.
  • Molecular weight: Not applicable to mixtures.
  • SMILES: Not applicable to mixtures.
  • InChIKey: Not applicable to mixtures.
  • CAS: Not applicable to mixtures.
  • CID: Not applicable to mixtures.
• Structural features (general)
  • No discrete 2D structure can be described; rather, a heterogeneous blend of amino acids/peptides, carbohydrates, minerals, and growth factors is present (literature/general).

Notes

• For precise lot-specific composition, performance parameters, and acceptance criteria, refer to the product CoA/Specification Sheet and the SDS.

Synthetic Utility

Not applicable. Mycoplasma Broth Medium (ChP) is a biological culture medium and is not used as a synthetic reagent or building block in chemical synthesis.

Guidance

• For chemical transformations, refer to dedicated reagents and solvents. This medium should be reserved for microbiological cultivation and enrichment workflows.
• If your project involves biocatalysis or cell-based synthesis, use this medium solely for growing mycoplasma in controlled research settings and not for producing chemical intermediates.

Target Specificity

This section is primarily relevant to antibodies and affinity reagents. It does not apply to a culture medium.

• Target/epitope, clone, isotype, species reactivity: Not applicable.

Practical note

• The “target” for this product in a functional sense is the broad support of Mycoplasma spp. growth during enrichment. Exact selectivity or inhibitory spectrum (if any) is Not specified for this item; refer to CoA/Spec Sheet and method guidance.

Domande frequenti

How should this product be stored?

Store at room temperature.