"Black‑goo contaminant" is a common cell‑culture contaminant that symbiotically coexists with cells and is passaged along with them. It competes with cells for growth resources, impairing cell proliferation and even causing cell death in severe cases. At present, "black‑goo contaminant" pollution occurs frequently in numerous cell lines, which severely compromises cell culture and subsequent downstream experiments.
The Nanobacteria Removal Agent by Aladdin has been validated on hundreds of cell lines, including mouse embryonic stem cells / iPS cells, human embryonic stem cells / iPS cells, HEK293, HeLa, HCT116, COS‑7, Vero, Huh‑7, MDCK, PANC‑1, SW620, U2OS, MCF‑7, MRC‑5 and NIH‑3T3. It exhibits no cytotoxicity to cells, can effectively eliminate most black‑goo contamination, and maintains normal cell viability for experiments.
Instructions for Use:
1. Upon receipt of the product, briefly centrifuge the reagent vial (3000 rpm, 3‑5 s) before storage.
2. For treatment with Nanobacteria Removal Agent, prepare the working solution freshly before use, and keep cell confluency at 50%‑60%.
3. The recommended dilution ratio is 1:1000. For example, add 10 μL of Nanobacteria Removal Agent into 10 mL culture medium.
Discard the old medium, wash the cells thoroughly with PBS, then add fresh culture medium supplemented with Nanobacteria Removal Agent. Refresh the medium every 2 days for one consecutive week.
Precautions:
1. This product shall not be mixed with antibiotics.
2. For personal safety and health, perform operations while wearing a lab coat and disposable gloves.
3. This product is intended for research‑only use by professionals. It must not be used for clinical diagnosis or treatment, nor for food‑ or pharmaceutical‑related applications.
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