Native-PAGE Loading Buffer (5X) - BioReagent, for western blot, for PAGE, 5X

Cat. No.: N752097

GRADE & PURITY BioReagent, for PAGE, for Western blot, 5X

Synonyms

Native Gel Sample Loading Buffer (5X)

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

WB

Overview

Native-PAGE Loading Buffer (reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into gel pores and indicating the movement position of protein samples in gel. The buffer does not contain denaturants such as SDS, but contains reducing agents such as DTT/β - mercaptoethanol. It is suitable for the preparation and electrophoresis of non denatured reduced protein samples.
Precautions：

1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution;
2. This product is only used for scientific research and cannot be used for human experiments or treatments;
3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.
Instructions for Use：

1. Reagent preparation
1.1 Mix the protein tracer loading buffer (non denaturing, 5x) with the protein sample in a ratio of 1:4;
2. Protein sample processing
2.1 Centrifuge the protein sample at less than 3000rpm for 30 seconds;
3. Protein loading and electrophoresis
3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole;
3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.

Specifications

Synonyms

Native Gel Sample Loading Buffer (5X)

Specifications & Purity

BioReagent, for western blot, for PAGE, 5X

Stability And Storage

Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.

Storage

Protected from light, Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for PAGE, for Western blot

Names and Identifiers

• PH: 6.2-7.2

Chemical and Physical Properties

• Sensitivity: Light sensitive