Native-PAGE Loading Buffer (5X) - BioReagent, for PAGE, for western blot, 5X

Cat. No.: N752097

QUALITÀ & PUREZZA BioReagent, for PAGE, for Western blot, 5X

Synonyms

Native Gel Sample Loading Buffer (5X)

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

WB

Panoramica

Native-PAGE Loading Buffer (reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into gel pores and indicating the movement position of protein samples in gel. The buffer does not contain denaturants such as SDS, but contains reducing agents such as DTT/β - mercaptoethanol. It is suitable for the preparation and electrophoresis of non denatured reduced protein samples.
Precautions：

1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution;
2. This product is only used for scientific research and cannot be used for human experiments or treatments;
3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.
Instructions for Use：

1. Reagent preparation
1.1 Mix the protein tracer loading buffer (non denaturing, 5x) with the protein sample in a ratio of 1:4;
2. Protein sample processing
2.1 Centrifuge the protein sample at less than 3000rpm for 30 seconds;
3. Protein loading and electrophoresis
3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole;
3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.

Specifiche

Sinonimi

Native Gel Sample Loading Buffer (5X)

Specifiche e purezza

BioReagent, for PAGE, for western blot, 5X

Stabilità e conservazione

Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.

Condizioni di conservazione di stoccaggio

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent, for PAGE, per il Western blot

Product Line

Prodotti biologici

Nomi e identificatori

PH

6.2-7.2

Proprietà chimiche e fisiche

• Sensibilità: Light sensitive