Neural-27 Supplement - sterile-filtered, for cell culture, BioReagent, sterile, 50×

Cat. No.: N778235

QUALITÀ & PUREZZA Sterile-filtered, BioReagent, for Cell culture, Sterile, 50×

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Specifiche

Specifiche e purezza

sterile-filtered, for cell culture, BioReagent, sterile, 50×

Stabilità e conservazione

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Condizioni di conservazione di stoccaggio

Protected from light,Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent, Filtrato asetticamente, per la coltura cellulare, Sterili

Product Line

Prodotti biologici

Proprietà chimiche e fisiche

• Sensibilità: Light-sensitive

Application Protocols

Item-specific, validated protocols are not provided for this product; refer to the CoA/Spec Sheet and SDS, and follow your laboratory’s SOPs.

• General guidance (literature; not a validated protocol for this item)
  • Thawing: Store at −20 °C protected from light. Thaw on ice or at 2–8 °C. Mix gently by inversion—avoid vigorous vortexing.
  • Aseptic handling: Work in a Class II biosafety cabinet. Disinfect exterior surfaces and use sterile, low-retention tips and tubes.
  • Aliquoting: Prepare single-use aliquots in sterile cryovials to avoid repeated freeze–thaw cycles. Refreeze promptly at −20 °C if not used immediately.
  • Dilution: Add the supplement to pre-warmed basal medium according to the manufacturer’s recommended ratio (not specified for this item). Mix by gentle swirling.
  • Quality checks: After preparation, inspect for turbidity/precipitate and confirm pH/osmolality of complete medium as required by your assay.

Any specific application parameters (e.g., recommended final concentration, compatible basal media, or validated cell types) are not specified for this item.

Biological Roles

Context: Neural culture supplements are designed to provide micronutrients and trophic support factors enabling serum-free growth and maintenance of neurons. The following describes typical roles in neuronal culture systems; it is not a composition statement for this specific item.

• General functions in neuronal culture (literature/general)

  • Antioxidant support: Vitamins and antioxidant molecules commonly mitigate oxidative stress, which is elevated in serum-free neuronal cultures.
  • Membrane and synapse support: Lipid-soluble factors and cholesterol carriers often aid membrane integrity and synaptogenesis.
  • Metabolic co-factors: Vitamins and defined nutrients sustain glycolytic and mitochondrial function.
  • Hormonal cues: Trace hormones (e.g., insulin-like factors) in some supplements assist survival signaling; exact components for this item are not specified.
  • Trace element balance: Defined minerals support enzymatic activity and neuronal excitability.

• Use cases (general)

  • Primary neuron maintenance from rodent or human sources under reduced-serum conditions.
  • Differentiation and maturation of neurons derived from pluripotent stem cells.
  • Support of neuron-like cell lines during functional assays where serum introduces variability.

• Critical considerations

  • Composition is supplement-specific; for this item, component identities and concentrations are not specified—consult the CoA/Spec Sheet.
  • Neuronal subtypes may respond differently; empirical optimization and careful monitoring (viability, morphology, electrophysiological properties) are recommended.

Buffer Applications

This product is not a buffering reagent and is not typically used to prepare analytical or biological buffers. Instead, it is added to pre-formulated basal media that supply buffering capacity (e.g., HEPES, bicarbonate systems in standard cell-culture media; general knowledge).

• Item-specific note

  • No buffer composition or pH capacity is specified for this item; refer to the CoA/Spec Sheet.

• Practical tip (general)

  • Verify the final pH and osmolarity of your complete medium after supplementation, particularly if using CO2-independent systems or custom buffer regimes.

Green Alternatives

Green chemistry framing is not directly applicable to a sterile biological supplement, but sustainable lab practices can reduce environmental impact.

• Practical sustainability measures (general)

  • Optimize shipping and storage: Consolidate orders and use manufacturer-recommended long-term storage (−20 °C) to minimize spoilage and repeat shipments. This item ships cold (ice chest + ice pads; item-specific), so plan receiving to avoid waste.
  • Reduce single-use plastics: Prepare appropriately sized aliquots to minimize tube consumption and product waste; use reusable cryoboxes and racks where possible.
  • Right-size experiments: Pilot small-scale tests to establish optimal dose and stability, preventing unnecessary media preparation and disposal.
  • Waste management: Dispose of expired media and supplement-containing waste according to institutional bio-waste guidelines; avoid mixing with chlorinated disinfectants if not required.

• Alternatives and trade-offs (general)

  • Some labs formulate in-house neuronal nutrient mixes from individual components to reduce packaging and cost. Trade-offs include increased labor, risk of variability, and sterility challenges compared with validated commercial supplements.

No composition-specific green substitutions are provided for this item; consult your institutional sustainability program for additional measures.

Pharmaceutical Uses

This product is intended for research use only in laboratory cell-culture workflows.

• Item-specific regulatory status

  • Research Use Only (from Product Data). Not intended for diagnostic procedures, therapeutic use, or as a GMP-manufactured raw material.

• Formulation/excipient context (general)

  • Cell-culture supplements of this class are sometimes analogously formulated as defined media additives in bioproduction research. Transition to clinical or manufacturing use would require GMP-grade sourcing and pharmacopeial or internal specifications; such status is not specified for this item.

• Documentation

  • No pharmacopeia monograph or excipient grade is specified for this item; refer to the CoA/Spec Sheet if manufacturing-grade documentation is required.

Physical Properties

• Item-specific facts (from Product Data)

  • Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Concentration/solvent system: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight / formula: Not applicable (mixture).

• Common physical characteristics (literature/general for neuronal supplements)

  • Typical form: Sterile-filtered liquid concentrate (e.g., 50×) or frozen aliquots intended for dilution into basal neuronal media. Specific concentration and appearance for this item are not specified.
  • pH/osmolality: Governed by the basal medium after dilution; concentrate pH is manufacturer-specific.

• Storage and stability (item-specific)

  • Store at −20 °C, protected from light (from Product Data).
  • Shipped in: Ice chest + ice pads (from Product Data).

Note: Properties such as refractive index, density, solubility, UV cutoff, and component assay values are not specified for this item; refer to CoA/Spec Sheet.

Quality and Grades

• Item-specific quality designations (from Product Data)

  • Sterile-filtered: Product has been membrane filtered (typically 0.2 µm) to remove microbial contaminants; preserves sterility during manufacturing.
  • BioReagent: Suitable for laboratory biological research. This indicates additional attention to bioburden, component quality, and performance in cell-based systems (not a GMP designation).
  • For Cell culture: Intended for use as a culture supplement; typically evaluated for sterility and compatibility with mammalian cell culture.
  • Sterile: Supplied as sterile; maintain aseptic technique to prevent contamination after opening.

• What these grades imply (general context)

  • Sterility: The product is provided sterile at release; sterility after opening depends on user handling. If endotoxin and mycoplasma specifications are critical, verify on the CoA/Spec Sheet (not specified here).
  • Lot-to-lot consistency: Cell-culture supplements are formulated to minimize variability; nevertheless, for sensitive neuronal applications, we recommend pilot testing new lots and documenting performance metrics (viability, morphology, electrophysiology where relevant).

• Documentation and testing

  • CoA/Spec Sheet should be consulted for any component disclosures, recommended dilution (e.g., 1× final), sterility/endotoxin test methods, and storage life. Any numerical specifications not listed here are not specified for this item.

Reaction and Applications

As a biological cell-culture supplement, this product is not used in synthetic organic reactions. Instead, it is applied to in vitro cell culture workflows.

• Cell-culture applications (general literature guidance; not item-specific claims)

  • Neuron support: Supplements of this class are formulated to support survival, maturation, and maintenance of primary neurons and neuron-like cells under serum-free or low-serum conditions.
  • Stem-cell workflows: Often incorporated during directed differentiation of pluripotent stem cells toward neural lineages and during maturation of neuron cultures prior to functional assays (e.g., electrophysiology, calcium imaging).
  • Disease modeling and screening: Used to stabilize culture conditions for neurobiology assays, toxicity testing, and phenotypic screens where serum-free, defined conditions are desired.

• Experimental design tips (general)

  • Titrate concentration: Start at the manufacturer-recommended dilution (not specified for this item) and evaluate a small matrix around that value to optimize for cell type and endpoints.
  • Control conditions: Include basal medium alone and, if relevant, serum-containing controls to benchmark viability and morphology.
  • Batch validation: Record media lot numbers and supplement lot numbers; maintain consistent feeding schedules, as antioxidant- and vitamin-rich supplements can degrade over time in incubator conditions.

Item-specific recommended use levels, component lists, or validated cell types are not specified for this item; refer to the CoA/Spec Sheet.

Reaction Conditions

Not applicable to chemical synthesis. For culture conditions, see below.

• Cell-culture conditions (general literature guidance; not item-specific)
  • Temperature/atmosphere: Typical mammalian neuronal cultures are maintained at 37 °C, 5% CO2, high humidity. The supplement is diluted into a compatible basal medium; specific dilution for this item is not specified.
  • Light sensitivity: Protect supplemented media from light to preserve stability of light-sensitive constituents.
  • Medium exchange: Partial medium changes (e.g., 25–50%) at defined intervals are common to sustain nutrient levels while retaining autocrine factors; optimize empirically for your system.

Item-specific use levels, compatible media, and stability in working solution are not specified for this item; consult the CoA/Spec Sheet.

Safety and Handling

• Regulatory note

  • Research Use Only (from Product Data). Not for human or veterinary use, diagnostic, or therapeutic applications.

• GHS and hazard information (item-specific)

  • Signal word / H-statements / Pictograms / GHS classification: Not specified for this item; refer to SDS.

• General laboratory safety (best practice for sterile cell-culture supplements; general guidance)

  • Handle in a certified biosafety cabinet (Class II) to maintain sterility and protect the work area.
  • Wear appropriate PPE: lab coat, gloves (nitrile), and safety glasses. Change gloves frequently to avoid contamination.
  • Avoid repeated freeze–thaw cycles to maintain product performance; prepare single-use aliquots upon receipt.
  • Protect from light during handling to minimize degradation of light-sensitive components (e.g., certain vitamins and antioxidants; general literature note).

• Incompatibilities and precautions (general)

  • Do not mix with undiluted strong acids/bases or oxidants; use only in compatible, sterile, cell-culture media systems.
  • If the supplement contains lipophilic factors or solvents (e.g., trace ethanol), ensure compatibility with your cells; composition for this item is not specified—consult the CoA.

• First aid (general)

  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing. Seek medical advice if irritation persists.
  • Ingestion/inhalation: Move to fresh air and seek medical attention as needed.

Refer to the product SDS for authoritative hazard, toxicology, and disposal guidance.

Solvent Selection

This product is a cell-culture supplement rather than a reaction solvent. Classical solvent selection does not apply.

• Item-specific note

  • Intended diluent: Cell-culture basal media (e.g., serum-free neuronal media) are typically used. For this item the exact medium compatibility is not specified; consult the CoA/Spec Sheet.

• Practical guidance (literature/general)

  • Common pairing: Neuronal supplements are often diluted into media such as Neurobasal/Neurobasal-A or DMEM/F12, with L-glutamine or GlutaMAX as appropriate. Verify osmolarity and pH of the final medium after supplementation.
  • Order of addition: Thaw supplement on ice, mix gently, and add aseptically to pre-warmed basal medium. Filter-sterilize the complete medium only if recommended and compatible with all components.
  • Avoid organic solvent carryover: If any component is solubilized in ethanol or other carriers (composition not specified for this item), ensure final concentrations remain within tolerated limits for your cells.

Storage and Reconstitution

• Item-specific storage conditions (from Product Data)

  • Store at −20 °C.
  • Protect from light.
  • Shipped in ice chest with ice pads to maintain cold chain.

• Stability and handling (general best practice)

  • Minimize freeze–thaw: Upon first receipt, thaw under controlled conditions (2–8 °C or on ice), mix gently, and aliquot into sterile, light-protective vials sized for single use. Repeated freeze–thaw can degrade activity of sensitive components (general literature note).
  • Working solution stability: Stability in complete medium depends on composition, temperature, and light exposure. Prepare fresh complete medium regularly and avoid prolonged exposure to ambient light. Specific in-medium stability for this item is not specified; consult the CoA/Spec Sheet.
  • Light protection: Use amber tubes or wrap containers in foil during handling and storage.

• Reconstitution/dilution

  • Physical form and recommended dilution are not specified for this item; refer to CoA/Spec Sheet.
  • If provided as a concentrate, dilute aseptically into sterile basal medium to the desired final concentration. Gently invert to homogenize; avoid foaming.

• Expiry

  • Shelf life, post-opening dating, and in-use dating are not specified for this item; refer to CoA/Spec Sheet and adhere to internal SOPs for time-in-use tracking.

Structure and Identity

Brief overview: Neural-27 Supplement is a complex, multi-component cell-culture supplement formulated for neuronal cell applications. It is not a single, well-defined small molecule and therefore lacks a single molecular structure.

• Item-specific facts (from Product Data)

  • Product name/alias: Neural-27 Supplement (SKU: N778235)
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey / SMILES: Not applicable (multi-component mixture); not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula / Molecular weight: Not applicable to a mixture; not specified for this item; refer to CoA/Spec Sheet.
  • Grade/Purity: Sterile-filtered, BioReagent, for Cell culture, Sterile.

• Structural identity considerations (general knowledge)

  • Composition: Cell culture supplements of this class are typically formulated as aqueous or aqueous/cryoprotectant concentrates containing defined micronutrients, vitamins, antioxidants, lipophilic factors and hormones; exact composition is product-specific and proprietary.
  • As a mixture, no single 2D/3D structure description applies. Components (if disclosed on the CoA) should be considered individually for structural representation and inventory management.

• Traceability & documentation

  • Use the product lot number to obtain the CoA for component listing (if disclosed), sterility testing results, endotoxin testing (if applicable), and recommended dilution. Without the CoA, conventional cheminformatics identifiers (SMILES/InChIKey) are not meaningful for this product class.

Synthetic Utility

Not applicable. Neural-27 Supplement is a biological culture additive, not a synthetic building block or reagent. It does not play a role in named organic reactions or retrosynthetic planning.

For relevant information, see the sections on Reaction & Applications (cell-culture context), Biological Roles, and Storage & Reconstitution.

Target Specificity

Not applicable. This product is not an antibody, probe, or ligand with target-binding specificity. No clone, isotype, antigen, or species-reactivity information applies.

Domande frequenti

What does sterile-filtered mean?

sterile-filtered indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.