🌎 Ship to: United States
Toggle Nav
Carrello
Cerca
Spedizione in giornata per tutti gli articoli disponibili in magazzino.
Ricerca avanzata
Ordine rapido SDS / CoA Structure Massivo e personalizzato
Menu
Account
Impostazioni
྾
224,000+ prodotti di ricerca · Triple ISO certified · COA & SDS Disponibile per ogni prodotto · Spedizione lo stesso giorno su articoli in magazzino

neuropeptide AF, CAS No.rp174604

CAS: rp174604 Cat. No.: rp174604
COA
neuropeptide AF, CAS No.rp174604
Disponibile su ordine
GRADE & PURITY Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
★
Size
Germania (EU)
USA*
Price
Qty
500μg
rp174604-500μg
Su ordinazione · 8–12 settimane
2.082,49€
1mg
rp174604-1mg
Su ordinazione · 8–12 settimane
3.470,87€
Enter a quantity for the sizes you want to add.
Bulk / Custom
♡ Lista dei desideri ⇅ Aggiungi al confronto ⎙ Print page
SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Specifications Stoccaggio e spedizione Documentazione Advanced Data Certificati (CoA, COO, BSE/TSE e tabella di analisi) Informazioni genetiche Calcolatori di soluzioni Recensioni Domande frequenti Application Protocols Biological Roles Buffer Applications Green Alternatives Pharmaceutical Uses Physical Properties Quality and Grades Reaction and Applications Reaction Conditions Safety and Handling Solvent Selection Storage and Reconstitution Structure and Identity Synthetic Utility Target Specificity
🧪
Why this grade

Moligand™ Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Room temperature Ships Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Product Name
neuropeptide AF, CAS No.rp174604
Sinonimi
NPAF
Grado
Moligand™
Specifiche e purezza
Moligand™
CAS
rp174604
Tipo di molecola
Peptidi
Product Line
Prodotti biologici
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Room temperature

Documentazione

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Informazioni genetiche
Alternate NamesNPAF
Reference
  • 1. Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration., The Journal of biological chemistry, Steegborn, C C and 7 more authors.
  • 2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences., Proceedings of the National Academy of Sciences of the United States of America, Strausberg, Robert L RL and 83 more authors.
  • 3. Genomic basis of cystathioninuria (MIM 219500) revealed by multiple mutations in cystathionine gamma-lyase (CTH)., Human genetics, Wang, Jian J and Hegele, Robert A RA.
  • 4. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase., Biochemical and biophysical research communications, Lu, Y Y, O'Dowd, B F BF, Orrego, H H and Israel, Y Y.
  • 5. Single nucleotide polymorphism in CTH associated with variation in plasma homocysteine concentration., Clinical genetics, Wang, J J, Huff, A M AM, Spence, J D JD and Hegele, R A RA.
  • 6. Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1., The Journal of biological chemistry, Yang, Guangdong G, Cao, Kun K, Wu, Lingyun L and Wang, Rui R.
  • 7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)., Genome research, Gerhard, Daniela S DS and 115 more authors.
  • 8. Towards a proteome-scale map of the human protein-protein interaction network., Nature, Rual, Jean-François JF and 37 more authors.
  • 9. The DNA sequence and biological annotation of human chromosome 1., Nature, Gregory, S G SG and 178 more authors.
  • 10. Polymorphisms in one-carbon metabolism and trans-sulfuration pathway genes and susceptibility to bladder cancer., International journal of cancer, Moore, Lee E LE and 14 more authors. more
Calcolatori di soluzioni
Recensioni

Recensioni dei clienti

Application Protocols

Tested applications, recommended dilutions, and positive controls: Not specified for this item; refer to CoA/Spec Sheet and the product catalog.

General note: As a research peptide ligand, typical workflows may include receptor binding assays, calcium flux, cAMP modulation, or electrophysiological recordings; however, no validated protocols are provided for this specific item. Users should optimize conditions (vehicle, concentration range, exposure time) for their particular cell line or tissue preparation.

Biological Roles

General literature context (not item-specific):

  • Neuropeptide AF (NPAF) is categorized within the RFamide peptide family, defined by a C‑terminal Arg–Phe–NH2 motif. RFamide peptides are neuropeptidergic signals broadly implicated in modulating neuronal activity and intercellular communication.
  • Reported roles in experimental systems include modulation of nociceptive signaling, cardiovascular and neuroendocrine functions, and synaptic transmission, often via G protein‑coupled receptors responsive to RFamide peptides (literature). The precise receptor interactions and downstream pathways can be species- and tissue-dependent.
  • Structure–activity: The C‑terminal RF‑amide is critical for receptor recognition; N‑terminal sequence variations tune potency/efficacy and receptor selectivity (literature). Amidation enhances receptor affinity and proteolytic stability relative to non‑amidated analogs.
  • Metabolism and stability: In biological matrices, peptidases (e.g., aminopeptidases, endopeptidases) can inactivate RFamide peptides; inclusion of protease inhibitors, rapid handling on ice, and use of serum‑free media can improve experimental signal windows.

Important limitations:

  • The specific sequence, receptor affinity, and potency of the product in this listing are Not specified for this item; consult the CoA/Spec Sheet and primary references associated with your application.

Compliance note:

  • For research use only (as stated in Product Data). No medical, diagnostic, or therapeutic uses are implied.
Buffer Applications

Applicability: As a peptide ligand, buffer choice directly affects solubility, stability, and bioassay performance.

General guidance (literature/practice; not item-specific):

  • Reconstitution: Begin with ultrapure water. If incomplete dissolution occurs, adjust to mildly acidic conditions (e.g., 0.1% acetic acid) or include minimal DMSO as a cosolvent prior to dilution into buffer.
  • Working buffers: Common choices include PBS, HBSS, or HEPES-buffered saline at pH 7.2–7.4 for cell-based assays; for biochemical assays, 10–50 mM HEPES or Tris at pH 7.2–7.5 with 100–150 mM NaCl is typical.
  • Additives: 0.05–0.1% BSA can reduce adsorption losses. Include protease inhibitors when working with tissue lysates or conditioned media to mitigate degradation.
  • Storage buffer: For short-term refrigerated storage, mildly acidic buffers (pH 4–6) can improve stability for many peptides; validate for your sequence. Avoid prolonged exposure to high pH.
  • Filtration: Use low protein binding filters (PES/RC) if sterile solutions are required.

Not applicable: Classical buffer systems as “pH standards” (e.g., phosphate/citrate calibration recipes) are not the focus here; instead, prioritize assay-compatible isotonic buffers and gentle handling. Item‑specific buffer compatibility and any stabilizers are Not specified for this item; refer to the CoA/Spec Sheet.

Green Alternatives

Applicability: As a peptide ligand, this product is typically handled in aqueous buffers, which inherently reduces reliance on volatile organic solvents. “Green alternatives” are thus focused on solvent systems and handling practices, not replacement chemicals.

Greener handling strategies (general):

  • Prefer water/PBS as the primary vehicle. Use minimal DMSO only to achieve initial dissolution, then dilute to the lowest feasible final percentage compatible with the assay.
  • Avoid TFA where possible; substitute with acetic acid or HEPES-buffered saline to lessen persistence and corrosivity, provided solubility and activity are maintained.
  • Utilize low-adsorption labware to reduce the need for surfactants or large carrier protein amounts.
  • Prepare higher-concentration master stocks to minimize container usage and shipment frequency, reducing packaging waste.

Comparison (general guidance):

  • Cosolvent choice:
    • DMSO: excellent solvency, but consider cytotoxicity at >0.5–1% and challenges in waste streams.
    • Acetonitrile: volatile and lower toxicity than many chlorinated solvents; use sparingly and capture vapors.
    • Ethanol: possible for some peptides but may destabilize certain assays; assess case-by-case.

Sustainability notes:

  • Store and ship at ambient when stability permits (this item: Storage Conditions = Room temperature, per Product Data), reducing cold-chain impact for the dry form.
  • Consolidate orders and use reusable secondary containment for spill prevention.

Always balance greener choices with data quality and peptide integrity.

Pharmaceutical Uses

This product is supplied for research use only (per Product Data) and is not intended for human or veterinary use.

Context (general, non-clinical):

  • Research peptides like Neuropeptide AF are employed in pre-formulation and discovery labs as tool ligands to characterize receptor systems, assess peptide stability, and benchmark analytical methods (e.g., LC–MS peptide mapping). These activities support scientific understanding but do not constitute pharmaceutical application of this specific item.
  • Excipient/formulation role: Not typical. Peptides of this class are active ligands rather than excipients and are not used as formulation aids.
  • Pharmacopeial status: No compendial status is implied. There is no USP/EP monograph linkage assumed for this item.

Operational notes for formulators (general, not item-specific):

  • If preparing model dosage forms for in vitro investigations, consider isotonic, pH‑controlled aqueous vehicles and minimize adsorption by using low‑binding containers or carrier proteins.
  • For stability studies, monitor degradation pathways (oxidation, deamidation, proteolysis) under ICH-like stress conditions to inform handling; results are sequence-dependent.

Summary: No pharmaceutical/clinical uses are claimed or implied for this item; it is intended solely as a research ligand within discovery and analytical laboratories.

Physical Properties

Item-specific physical data:

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Melting point, boiling point, density, refractive index, pKa, logP/logD, solubility: Not specified for this item; refer to CoA/Spec Sheet.

General/literature context for peptide materials (not item-specific):

  • Physical state: Peptides are typically supplied as lyophilized solids or films, often off‑white to white.
  • Hygroscopicity: Many peptides are hygroscopic; exposure to ambient humidity can lead to clumping or hydrolysis over time.
  • Solubility behavior: Solubility depends strongly on sequence and terminal modifications. RFamide peptides often dissolve in aqueous buffers with mild acid (e.g., 0.1% TFA or acetic acid) or in polar aprotic cosolvents (DMSO) before dilution with water or saline (literature).
  • Charge state: The presence of a C‑terminal amide and basic side chains (e.g., Arg) typically drives net positive charge under neutral to mildly acidic conditions, improving aqueous compatibility (literature).

Practical note: Establish solubility empirically on a small scale using a stepwise approach (water → dilute acid → aqueous with minimal DMSO). Record the working stock concentration and vehicle to ensure reproducibility. Always verify item-specific specifications on the CoA/Spec Sheet.

Quality and Grades

Item-specific: Grade/Purity = Moligand™ (from Product Data).

Interpretation and implications (general description of this grade):

  • Moligand™ indicates inclusion in a small‑molecule/ligand screening collection intended for discovery workflows (e.g., receptor/target profiling, phenotypic assays). Emphasis is typically on authenticity, fitness for bioassay, and batch traceability rather than pharmacopeial compliance.
  • Documentation: Expect batch CoA with identity confirmation (e.g., LC–MS/HRMS, HPLC area %), where applicable. Exact analytical methods and acceptance criteria are item‑specific and should be verified on the CoA/Spec Sheet.
  • Use case alignment: Optimized for in vitro screening, hit validation, and mechanistic studies. UV background and extractables are generally appropriate for plate‑based assays and LC readouts, though users should confirm compatibility with their detection modality.

What is not implied:

  • This grade does not imply GMP manufacturing, compendial (USP/EP) monograph compliance, or suitability for human/clinical use.

Best practices:

  • Verify batch identity and purity on receipt if your workflow is purity‑sensitive (e.g., biophysics). If needed, perform additional polishing (e.g., HPLC) prior to biophysical measurements.
  • Record solvent/vehicle, concentration, and storage history for data integrity.

Note: When item-specific specifications (e.g., purity %, residual solvents, water content) are required, consult the CoA/Spec Sheet; they are Not specified for this item in the current listing.

Reaction and Applications

This item is a research peptide ligand rather than a synthetic reagent; it is not typically used as a substrate or catalyst in organic reactions. Accordingly, classical synthetic reaction families (e.g., cross-coupling, Grignard) are not applicable.

Research applications (general/literature context for Neuropeptide AF/RFamide peptides):

  • GPCR pharmacology: Employed as a tool ligand in receptor binding and functional assays that interrogate RFamide-responsive receptors. Useful for potency, efficacy, and signaling pathway profiling in heterologous expression systems or native tissues.
  • Neuroscience and physiology research: Applied in ex vivo tissue bath experiments, electrophysiology, and cellular assays to probe peptide-mediated modulation of neuronal excitability and transmitter release (literature). No clinical/therapeutic claims.
  • Assay development: Serves as a positive control or reference agonist/ligand in high-content or high-throughput screening formats. Suitable for concentration–response studies and orthogonal assay validation.

Practical tips (general best practice):

  • Prepare concentrated, clarified stocks (e.g., in water or minimal DMSO) and perform serial dilutions in assay buffer to minimize adsorption and vehicle effects.
  • Include carrier protein (e.g., 0.1% BSA) when nonspecific adsorption to plastics is observed.
  • Minimize light/oxidant exposure; consider adding antioxidant (e.g., 0.1 mM methionine) for oxidation‑prone sequences during extended incubations.

Note: Item‑specific sequence, potency, and receptor selectivity are Not specified for this item; consult the CoA/Spec Sheet or primary literature for the exact analog in use.

Reaction Conditions

Classical chemical reaction conditions are not applicable to this peptide ligand. Instead, consider assay and handling conditions relevant to biological testing (general literature guidance; not item-specific):

  • Stock preparation: 1–10 mM stocks in water or minimal DMSO; verify clarity and absence of particulates. Filter sterilize if needed (0.22 μm, low‑binding membranes).
  • Working concentrations: Typically nM–μM in cellular/biochemical assays; determine empirically via concentration–response curves using your receptor/cell system.
  • Temperature: Prepare and store working solutions on ice when feasible; conduct biological assays at 25–37°C as required by the system.
  • Buffer: Use assay‑compatible buffered saline (e.g., HEPES or PBS) at physiological pH; include BSA (0.05–0.1%) to limit adsorption when needed.
  • Stability window: Limit time in aqueous solution at room temperature; aliquot stocks and freeze for longer-term storage to avoid multiple freeze–thaw cycles.

Expected outcomes and yields: Not applicable in a chemical synthesis sense. For bioassays, report responses as Emax/EC50/IC50 with appropriate replicates and controls.

Note: Item-specific potency, receptor subtypes, and binding kinetics are Not specified for this item; consult primary literature and the CoA/Spec Sheet to tailor exact conditions.

Safety and Handling

Regulatory/GHS (item-specific):

  • Signal word: Not specified for this item; refer to SDS.
  • Hazard statements (H‑statements): Not specified for this item; refer to SDS.
  • GHS classification and pictograms: Not specified for this item; refer to SDS.

General laboratory safety for peptides (literature/best practice):

  • Handle as a laboratory chemical. Avoid inhalation of powders and contact with skin/eyes. Use appropriate PPE: lab coat, safety glasses, and disposable nitrile gloves.
  • Work in a clean, dry area; prevent aerosolization when opening lyophilized vials (allow vial to equilibrate to room temperature before opening to reduce condensation and clumping).
  • Avoid repeated freeze–thaw of reconstituted solutions to prevent degradation or aggregation.
  • Incompatibilities: Strong oxidizers may degrade susceptible residues (Met, Cys, Trp). Elevated pH and high temperatures can accelerate deamidation/oxidation and peptide bond hydrolysis.
  • First aid (overview): If inhaled, move to fresh air; if on skin, wash with soap and water; if in eyes, rinse cautiously with water for several minutes; if ingested, rinse mouth. Seek medical attention if symptoms persist. Defer to SDS for authoritative guidance.

Waste: Dispose of unused material, solutions, and contaminated consumables according to institutional chemical waste procedures. Do not release to drains unless permitted.

Research use: For research use only (as stated in Product Data); not for diagnostic or therapeutic use.

Solvent Selection

Item-specific solubility data: Not specified for this item; refer to CoA/Spec Sheet.

General guidance for RFamide-class peptides (literature/practice):

  • Primary solvents: Start with high‑purity water or PBS. If insoluble, acidify slightly (e.g., 0.1% acetic acid or 0.1% TFA in water) to protonate basic residues and improve dissolution.
  • Cosolvents: DMSO can be used to prepare concentrated stocks (e.g., 10–50 mM) for hydrophobic sequences, then dilute into aqueous media to ≤0.1–1% v/v DMSO in assays, as compatible.
  • Alternative vehicles: 10–20% acetonitrile in water with 0.1% acid can assist dissolution for some sequences; immediately dilute into buffer to assay-compatible organic content.
  • pH considerations: Maintain pH that preserves integrity (often pH 4–7 for storage; adjust to assay pH immediately before use). Avoid high pH for prolonged periods to limit deamidation/isoaspartate formation.
  • Filtration: If needed, filter through low‑protein‑binding membranes (PTFE for organic content, PES/RC for aqueous) to remove particulates.

When to choose alternatives:

  • If the peptide remains intractable in water, prepare a minimal DMSO stock first, then titrate aqueous buffer while monitoring clarity.

Compatibility snapshot (general):

  • Miscible with: water, saline buffers; conditionally with DMSO/MeCN as cosolvents.
  • Incompatible with: strong oxidizers; prolonged exposure to high base; high % organic in cell-based assays.

Always confirm final vehicle compatibility with the biological readout.

Storage and Reconstitution

Item-specific storage (from Product Data):

  • Storage Conditions (dry material): Room temperature.
  • Shipped In: Not specified for this item; refer to CoA/Spec Sheet.

General best practices for peptides (not item-specific):

  • Upon arrival, allow the sealed container to equilibrate to room temperature before opening to avoid condensation. Keep desiccated and protected from light.
  • Long-term dry storage: Stable at ambient when dry if kept tightly closed with desiccant; for extended periods, some users prefer 2–8°C as an added precaution—ensure consistency with any stability data supplied on the CoA.

Reconstitution (general guidance):

  • Solvent choice: Start with sterile water. If needed, use 0.1% acetic acid or minimal DMSO to aid dissolution, then dilute with buffer (e.g., PBS or HEPES) to the desired concentration.
  • Concentration: Prepare a concentrated stock solution to minimize storage container count. Record exact vehicle and concentration.
  • Aliquoting and solution storage: Dispense single‑use aliquots; store frozen (−20 to −80°C) to minimize freeze–thaw. Avoid frost-free freezers. Typical solution stability is sequence- and vehicle-dependent; verify empirically.
  • Thawing: Thaw on ice and mix gently; do not vortex aggressively to avoid foaming/adsorption.

Note: Item-specific solubility, sequence, and stability in solution are Not specified for this item. Refer to the CoA/Spec Sheet and SDS for authoritative guidance.

Structure and Identity

Brief description: Neuropeptide AF (often abbreviated NPAF) is a research peptide ligand commonly used in neuropeptide/GPCR screening collections.

Item-specific identifiers (from Product Data):

  • SKU: rp174604
  • Product name: neuropeptide AF
  • Grade/Purity: Moligand™
  • CAS: rp174604 (catalog identifier; not a registry CAS)
  • InChIKey: 443149 (as provided)
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general literature context, not item-specific):

  • Neuropeptide AF belongs to the RFamide peptide family, characterized by a C‑terminal Arg–Phe–NH2 motif.
  • Typical members are short to medium-length peptides (8–26 residues) with an amidated C‑terminus; the amidation is often critical for bioactivity (literature).
  • 2D description in words: a linear peptide backbone of L‑amino acids terminating in –Arg–Phe–CONH2, with side chains presenting mixed polar, basic, and hydrophobic functionalities. The presence/position of basic residues (e.g., Arg/Lys) and aromatics (Phe/Trp/Tyr) define charge/hydrophobic patches (literature).

Note: Exact sequence, stereochemistry, and any modifications for this specific item are not provided in the Product Data. Consult the CoA/Spec Sheet for definitive structural identity.

Synthetic Utility

Not typically applicable. Neuropeptide AF is a peptide ligand used as a biological probe rather than a synthetic reagent or building block in classical organic synthesis.

General context (literature):

  • While peptides can, in principle, serve as templates or catalysts in specialized settings, RFamide neuropeptides are primarily utilized to interrogate biological signaling rather than to drive chemical transformations.
  • If derivatization is desired for research (e.g., fluorescent tagging, biotinylation), standard peptide conjugation chemistries (NHS ester acylation of Lys side chains, maleimide coupling to Cys if present, or N‑terminal oxime/aldehyde chemistry) may be employed. These modifications are sequence‑dependent and outside the scope of the supplied item unless explicitly specified on the CoA.

Item-specific reactivity or protected functionalities are Not specified for this item; refer to CoA/Spec Sheet before attempting any chemical modification.

Target Specificity

Not an antibody product; classical antigen/epitope/clone specificity does not apply.

General literature context (not item-specific):

  • RFamide peptides, including Neuropeptide AF, are studied as ligands for RFamide‑responsive receptors (GPCRs). Receptor subtype preferences and efficacies are sequence- and species-dependent and must be determined experimentally in the user’s system.

Item-specific target details (receptor name, EC50/IC50, species reactivity) are Not specified for this item; refer to CoA/Spec Sheet and primary literature relevant to your assay.

Domande frequenti

What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
How should this product be stored?
Store at room temperature.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is rp174604. InChIKey YPBGORNZMUQFSF-KWFINLGMSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Moligand™ grade guide →
Bulk & Custom Packaging Need larger quantities or custom specifications? Contact our team for a tailored quote.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.

Privacy Policy Terms of Use Cookie Policy Terms and Conditions of Sale Sales Policy
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Copyright © 2023–present Aladdin Scientific Corp. All rights reserved.