GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Endotoxin Free?Endotoxin-free — verified below sensitive endotoxin limits. Use in cell culture, in-vivo, and immunology work where endotoxin triggers responses.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofac
Specie ospite
Mouse
Specificità
Human NQO1
Immunogeno
Recombinant full length protein corresponding human NQO1
Controllo positivo
WB: HepG2, MCF-7, HeLa and A549 whole cell lysates Flow Cytometry: MCF-7 cells IF/ICC: MCF-7 cells
Coniugazione
Unconjugated
Grado
Carrier Free, Endotoxin Free, ExactAb™, High Performance, PBS Only, Validated
Tipo di catena leggera
kappa
SDS-PAGE
150.0 KDa
Metodo di purificazione
Protein A purified
Concentrazione
1.0 mg/mL
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
NQO1 Mouse mAb (Ab224768) - Western Blot All lanes: NQO1 Mouse Antibody (Ab224768) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 31 kDa Observed band size: 30 kDa Exposure time: 30.0 s
NQO1 Mouse mAb (Ab224768) - Flow Cytometry Overlay histogram showing MCF-7 cells stained with NQO1 Mouse mAb (Ab224768) (Red) at 1.0 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).
NQO1 Mouse mAb (Ab224768) - IF IF analysis of NQO1 (Green) in MCF-7 cells. The cells were fixed with 4% PFA for 15 minutes and then 0.3% Triton X-100 for 15 minutes at RT. Cells were stained with NQO1 Mouse mAb (Ab224768) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Applicazione
Applicazione
Dilution info
IF/ICC
1/200
WB
1/1000 - 1/200000
Flow Cytometry
1/1000
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Recensioni
Recensioni dei clienti
Domande frequenti
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
What does Endotoxin Free mean?
Endotoxin Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (NQO1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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