GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Endotoxin Free?Endotoxin-free — verified below sensitive endotoxin limits. Use in cell culture, in-vivo, and immunology work where endotoxin triggers responses.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofac
Host species
Mouse
Specificity
Human NQO1
Immunogen
Recombinant full length protein corresponding human NQO1
Positive Control
WB: HepG2, MCF-7, HeLa and A549 whole cell lysates Flow Cytometry: MCF-7 cells IF/ICC: MCF-7 cells
Conjugation
Unconjugated
Grade
Carrier Free, Endotoxin Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
NQO1 Mouse mAb (Ab224768) - Western Blot All lanes: NQO1 Mouse Antibody (Ab224768) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 31 kDa Observed band size: 30 kDa Exposure time: 30.0 s
NQO1 Mouse mAb (Ab224768) - Flow Cytometry Overlay histogram showing MCF-7 cells stained with NQO1 Mouse mAb (Ab224768) (Red) at 1.0 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).
NQO1 Mouse mAb (Ab224768) - IF IF analysis of NQO1 (Green) in MCF-7 cells. The cells were fixed with 4% PFA for 15 minutes and then 0.3% Triton X-100 for 15 minutes at RT. Cells were stained with NQO1 Mouse mAb (Ab224768) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Application
Application
Dilution info
IF/ICC
1/200
WB
1/1000 - 1/200000
Flow Cytometry
1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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