NUCB2 Mouse mAb - Primary antibody, specific to NUCB2

Cat. No.: Ab118866

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

K70044_2H5

Conjugation

Unconjugated

Overview

Mouse anti Human NUCB2 Antibody, Monoclonal (K70044_2H5), could be used for WB, IHC, IP and so on.

Specifications

Product Name

NUCB2 Mouse mAb - Primary antibody, specific to NUCB2

Synonyms

DNA binding protein NEFA antibody | DNA-binding protein NEFA antibody | Gastric cancer antigen Zg4 antibody | HEL S 109 antibody | NEFA antibody | Nefastin 1 antibody | Nefastin-1 antibody | Nefastin1 antibody | Nucb2 antibody | NUCB2_HUMAN antibody | Nuc

Specifications & Purity

ExactAb™, Carrier Free, Validated, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Calcium-binding protein. May have a role in calcium homeostasis.

Host species

Mouse

Specificity

NUCB2

Immunogen

Recombinant protein of human NUCB2 (AA 31-410)

Positive Control

WB: HL-60 lysates. IHC: Human stomach tissue. IP: HL-60 lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: NUCB2 Mouse mAb(Ab118866)

NUCB2 Mouse mAb(Ab118866) - Western Blot
All lanes: NUCB2 Mouse mAb(Ab118866) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 100.0 s

IHC: NUCB2 Mouse mAb(Ab118866)

NUCB2 Mouse mAb(Ab118866) - IHC
IHC-P analysis of stomach tissue by NUCB2 Mouse mAb(Ab118866).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded stomach tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NUCB2 Mouse mAb(Ab118866) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells are positively stained at the cytoplasm.

IP: NUCB2 Mouse mAb(Ab118866)

NUCB2 Mouse mAb(Ab118866) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of NUCB2 Mouse mAb(Ab118866) with HL-60 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NUCB2 Mouse mAb(Ab118866) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was mouse IgG1.
Lane 1: HL-60 lysate
Lane 2: NUCB2 immunoprecipitated from HL-60 lysate by NUCB2 Mouse mAb(Ab118866)
Lane 3: The same as Lane 2 was used as IgG isotype control antibody
Result: NUCB2 Mouse mAb(Ab118866) can immunoprecipitate NUCB2.

Application

• Application: Dilution info
• IP:

  1/100;

• IHC:

  1/200 ;

• WB:

  1/500;

Associated Targets(Human)

NUCB2 Tbio Nucleobindin-2 (0 Activities)

Discover Target: NUCB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)