PBS-EDTA Buffer (4×, pH 7.4, Sterile) - BioReagent,for cell culture,sterile,4×

Cat. No.: A1509645

GRADE & PURITY BioReagent, for Cell culture, Sterile, 4×

Synonyms

PBS-EDTA Buffer

Storage

Room temperature

Shipped In

Normal

Panoramica

  Balanced Salt Solution (BSS) matches the environmental conditions such as pH value and osmotic pressure required for cell growth. It functions to maintain osmotic pressure, regulate acid-base balance, and supply energy and inorganic salt components essential for cell survival and metabolism. BSS can meet the basic needs of cell survival and sustained metabolism in in vitro experiments. It is mainly composed of inorganic ions, and sometimes contains sodium bicarbonate, glucose, phenol red, etc. If necessary, HEPES can also be added to maintain osmotic pressure balance. Balanced Salt Solution can be used as the base solution of complete medium, or for diluting concentrated amino acid and vitamin solutions to prepare complete medium. The formulation of BSS is often modified. For example, Hank's BSS is available in variants without calcium, magnesium or phenol red, as well as those containing calcium, magnesium and phenol red; Dulbecco's PBS also has versions with or without calcium and magnesium. HBSS, EBSS, PBS and others are all salt solutions related to weakly buffered phosphate solutions. Common balanced salt solutions include Earle's solution, Hank's solution, Phosphate-Buffered Saline (PBS) and so on.

  PBS-EDTA Buffer (4×PE, pH 7.4) is one of the commonly used phosphate-buffered solutions. It is mainly composed of EDTA, Na₂HPO₄, NaH₂PO₄, etc., with a pH value of approximately 7.4. This reagent has undergone autoclaving sterilization. It is often used for chelating metal ions (especially Ca²⁺, Mg²⁺) and can also be applied in the detection of nuclear import of substances. This reagent is a 4× concentrated solution and should be diluted to 1× working concentration before use. This reagent is for research use only and not intended for clinical diagnosis or any other purposes.

Operating Procedures (for reference only)

1. Operate according to the specific requirements of the experiment.

2. It should generally be diluted to 1× working concentration before use.

Precautions

1. Ensure aseptic operation to avoid microbial contamination.

2. This reagent has been autoclaved. Please use the reagent as soon as possible after opening to prevent affecting the results of subsequent experiments.

3. For your safety and health, wear a lab coat and disposable gloves during operation.

Specifications

Sinonimi

PBS-EDTA Buffer

Specifiche e purezza

BioReagent,for cell culture,sterile,4×

Stabilità e conservazione

Store at room temperature long term (12 months).

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Grado

BioReagent, per la coltura cellulare, Sterili

Nomi e identificatori

• PH: 7.4

Domande frequenti

What does for cell culture mean?

for cell culture indicates that the product has been processed and tested for cell culture and tissue engineering. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.