GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote
Specie ospite
Mouse
Specificità
PCNA
Immunogeno
Full length recombinant human PCNA
Controllo positivo
IF/ICC: Hela cells Flow Cytometry: Hela cells
Coniugazione
AF405
Eccitazione (nm)
Violet Laser(405nm)
Emissione (nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grado
ExactAb™, Validated
Tipo di catena leggera
kappa
Concentrazione
0.5 mg/mL
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Immagini
PCNA Mouse mAb (AF405) (Ab219140) - ICC/IF IF analysis of PCNA (Blue) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (AF405) (Ab219140) at a dilution of 1/1000 in blocking buffer overnight at 4°C. Cells were counterstained with PI (Red). Images were taken on the confocal laser scanning microscope.
PCNA Mouse mAb (AF405) (Ab219140) - Flow Cytometry Flow cytometry analysis of PCNA expression by Hela cells (Red) compared to an isotype control (Blue). Cells were fixed and permeabilized with 100% methanol and then stained with PCNA Mouse mAb (AF405) (Ab219140) (Red) at 1/150 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF405) (Ab190076).
Applicazione
Applicazione
Dilution info
IF/ICC
1:50-1:500
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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