GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.PCR Reagent?PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification.Sterile?Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization.10×
Synonyms
PCR Reaction Buffer (10×, pH 8.3)
Storage
Store at -20°C,Avoid repeated freezing and thawing
BioReagent,PCR Reagent,sterile,10× BioReagent,PCR Reagent,Sterili for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
PCR Amplification Buffer (10×, pH 8.3) is a core auxiliary reagent for the Polymerase Chain Reaction (PCR). It provides a stable pH environment and necessary ionic conditions for the PCR reaction, ensures the activity of Taq DNA polymerase and other PCR-related enzymes, promotes the specific binding of primers to template DNA, and thereby facilitates the efficient amplification of target fragments.
The main components of this product include 100 mM Tris-HCl buffer (pH 8.3), 15 mM Mg²⁺, and 500 mM KCl, among others.
Procedure (For Reference Only)
Operate according to the specific requirements of the experiment.
PCR Amplification Buffer (10×, pH 8.3) should be used at a working concentration of 1×.
Notes
To avoid a decrease in amplification efficiency, repeated freezing and thawing should be avoided.
Prevent microbial contamination.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This product is for research use only and is strictly prohibited for other purposes.
Specifications
Sinonimi
PCR Reaction Buffer (10×, pH 8.3)
Specifiche e purezza
BioReagent,PCR Reagent,sterile,10×
Stabilità e conservazione
Store at -20℃ long term (12 months). Avoid freeze/thaw cycle.
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.
Grado
BioReagent, PCR Reagent, Sterili
Nomi e identificatori
PH
8.3
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
What does PCR Reagent mean?
PCR Reagent indicates that the product has been processed and tested for PCR, sequencing and nucleic acid amplification. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.