PECI/ECI2 Mouse mAb - Anticorpo primario, specific to ECI2

Cat. No.: Ab121138

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG2a

Clone Number

K16256_15A10

Conjugation

Unconjugated

Panoramica

Mouse anti Human PECI/ECI2 Antibody, Monoclonal (K16256_15A10), could be used for WB, IHC, IP and so on

Specifications

Product Name

PECI/ECI2 Mouse mAb - Anticorpo primario, specific to ECI2

Sinonimi

2-trans-enoyl-CoA isomerase | ACBD2 | acyl Coenzyme A binding domain containing 2 | D2-enoyl-CoA isomerase | D3 | D3 D2 enoyl CoA isomerase | DBI related protein 1 | DBI-related protein 1 | delta(2)-enoyl-CoA isomerase | Delta(3) | Delta(3) delta(2) enoyl

Specifiche e purezza

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Meccanismi biochimici e fisiologici

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.

Specie ospite

Mouse

Specificità

ECI2

Immunogeno

Recombinant protein of Human PECI/ECI2 (AA 41-390)

Controllo positivo

WB: Raji, Jurkat and K562 cell lysates. IHC: Human liver tissue. IP: Raji lysates.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -80°C,Avoid repeated freezing and thawing

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: PECI/ECI2 Mouse mAb (Ab121138)

PECI/ECI2 Mouse mAb (Ab121138) - Western Blot
All lanes: PECI/ECI2 Mouse mAb (Ab121138) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39, 35 kDa
PECI/ECI2 Mouse mAb (Ab121138) recognizes two isoforms of PECI/ECI2.
Exposure time: 109.1 s

IP: PECI/ECI2 Mouse mAb (Ab121138)

PECI/ECI2 Mouse mAb (Ab121138) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of PECI/ECI2 Mouse mAb (Ab121138) with Raji lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PECI/ECI2 Mouse mAb (Ab121138) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.
Lane 1: Raji lysate
Lane 2: ECI2 immunoprecipitated from Raji lysate by PECI/ECI2 Mouse mAb (Ab121138)
Lane 3: The same as Lane 2 but mouse lgG was used as IgG isotype control antibody
Result: PECI/ECI2 Mouse mAb (Ab121138) can immunoprecipitate ECI2.

IHC: PECI/ECI2 Mouse mAb (Ab121138)

PECI/ECI2 Mouse mAb (Ab121138) - IHC
IHC analysis of liver tissue by PECI/ECI2 Mouse mAb (Ab121138).
IHC was performed using sections of the formalin-fixed paraffin-embedded liver tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PECI/ECI2 Mouse mAb (Ab121138) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Hepatocytes are positively stained at the cytoplasm.

Applicazione

Applicazione

Dilution info

WB

1/1000

IP

1/100

IHC

1/200

Obiettivi associati (umani)

ECI2 Tbio Enoyl-CoA delta isomerase 2, mitochondrial (0 Activities)

Discover Target: ECI2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PECI/ECI2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.