GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
The degree of pectin methylesterification (PMD) determines the number of negative charges and is closely related to the adsorption of heavy metals by plant root cell walls and the resistance to heavy metal toxicity. The pectin content of the cell wall and its degree of methylesterification directly affect the plant's ability to withstand heavy metal toxicity. In the assay, pectin methyl ester bonds are released by saponification to generate methanol, which is oxidized by alcohol oxidase to formaldehyde. Formaldehyde then reacts with 2,4-pentanedione to produce a colored product with a characteristic absorbance peak at 412 nm. Meanwhile, galacturonic acid undergoes a condensation reaction with carbazole in a sulfuric acid solution to form a purple-red product with a characteristic absorbance peak at 530 nm. The degree of pectin methylesterification (PMD) is calculated as the ratio of methanol production to galacturonic acid content.
Specifications
Specifiche e purezza
BioReagent
Grado
BioReagent
Detection instrument
Microplate reader
Detection wavelength
412mm,530nm
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at 2-8°C
Spedito in
Wet ice
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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