Pectic substances, existing in the forms of protopectin, pectin, and pectic acid, are widely distributed in plant fruits, roots, stems, and leaves. As a cell wall component associated with cellulose, they act as the adhesive in the middle lamella between adjacent cells, contributing to the tight bonding of plant tissue cells. Among these, protopectin is water-insoluble. In unripe fruits or plant tissues, pectic substances are often bound to cellulose in the form of protopectin, lending rigidity and brittleness to the plant tissue. Protopectin can be hydrolyzed by chemical reagents such as acids, alkalis, salts, or by enzymes, converting into water-soluble pectin. Pectin, also known as polygalacturonic acid, is a linear polysaccharide polymer composed of D-galacturonic acid residues linked by α-1,4 glycosidic bonds. It contains several hundred to about 1000 dehydrated galacturonic acid residues, with an average relative molecular mass ranging from 50,000 to 150,000.
This product is based on the ferric chloride-hydroxylamine reaction for staining. After staining, pectin appears red. It is primarily suitable for staining samples such as fresh plant sections, paraffin sections, and free-hand sections. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
P1511526
| Component
| 4×50 mL
| Storage
|
P1511526A
| Acid Enhancer Solution
| 50 mL
| RT. |
P1511526B
| Hydroxylamine Solution
| 25 mL
| RT.
|
P1511526C
| Alkaline Solution
| 25 mL
| RT. Store in the dark. |
P1511526D
| Acidification Solution
| 50 mL
| RT.
|
P1511526E
| Ferric Chloride Solution
| 50 mL
| RT. |
Before use, mix Hydroxylamine Solution and Alkaline Solution at a 1:1 ratio to prepare the Alkaline Hydroxylamine Staining Solution. Prepare fresh for immediate use.
Materials to Be Prepared by User
- Glass slides
- Graded ethanol series
Procedure (For Reference Only)
1. Sectioning: Use fresh tissue sections, free-hand sections, or sections from a sliding microtome, and place them on a glass slide.
2. Enhancement: Add pre-warmed (37–45°C) Acid Enhancer Solution to cover the section. Allow it to stand at room temperature for 1-3 minutes.
3. Discard the solution and rinse briefly under running water.
4. Alkalinization: Add 250-500 μL of Alkaline Hydroxylamine Staining Solution to completely cover the section. Allow it to stand at room temperature for 5 minutes or longer.
5. Acidification: Directly add an equal amount of Acidification Solution. Allow it to stand at room temperature for 2 minutes.
6. Remove the liquid and blot dry with filter paper. Add Ferric Chloride Solution to immerse the section. Allow it to stand at room temperature for 3-8 minutes.
Staining Results
Notes
1. Staining results are generally suboptimal for paraffin sections. Ensure paraffin removal is as thorough as possible.
2. Avoid the use of chromic acid fixatives, as chromate treatment can also interfere with iron retention.
3. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
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