Penicillinase, CAS No.9001-74-5

CAS: 9001-74-5 Cat. No.: P1523063 Numero EC: 232-628-8

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, Sterile, ≥10MU/EA; expressed in E.coli

Accession #

P00809

Expression system

E. coli

Bioactivity

≥10MU/EA

Panoramica

Penicillinase, also known as Type I β-lactamase (EC 3.5.2.6), is capable of hydrolyzing the β-lactam ring of β-lactam antibiotics, thereby rendering them devoid of antimicrobial activity. This product is a recombinant penicillinase produced by genetic engineering technology. It is prepared by constructing a high-efficiency expression engineered strain for penicillinase, followed by fermentation and purification to obtain a product with high purity and high activity. The product features high substrate specificity. It can effectively hydrolyze β-lactam antibiotics, including Penicillin G, Ampicillin, Amoxicillin, Pivampicillin, Carbenicillin, Ticarcillin, Furbenicillin, Mezlocillin, etc.

Scope of Application

It is applicable to the sterility test and microbial limit test of raw materials and preparations of β-lactam antibiotics, namely the Sterility Test (General Principles of Volume IV, Chinese Pharmacopoeia 2025 Edition) and Microbial Limit Test (Appendices & General Principles of Volume IV, Chinese Pharmacopoeia 2025 Edition) as specified in the Chinese Pharmacopoeia. It can also be used as a reagent for sterility test specified in USP-NF General Chapters <71> Sterility Test (USP 29).

Method of Use

1. Membrane Filtration Method

(1) Dissolve one vial of the product in 1 mL of pre-cooled sterile water, then dilute to 1000 mL with sterile water to prepare the rinsing solution. Sterilize all required laboratory apparatus by appropriate methods and soak them in the rinsing solution for 10 minutes. Avoid vigorous shaking during dissolution and prevent the generation of excessive air bubbles.

(2) Take the specified amount of test sample, perform filtration in accordance with the specified procedure, and rinse the membrane filter with an appropriate volume of the rinsing solution prepared in step (1).

(3) Dilute another 1 mL of penicillinase with sterile water to 200 mL. Add 10 mL of this solution to each culture medium, and store the remaining solution at 2–8°C for later use.

(4) Inoculate the membrane filter into the culture medium following the specified procedure.

2. Direct Inoculation Method

Take the specified amount of test sample (as stipulated by the Pharmacopoeia), mix well, add an appropriate volume of sterile penicillinase solution (as stipulated by the Pharmacopoeia), and inoculate into each tube of culture medium. Alternatively, inoculate the test sample directly into the culture medium containing sterile penicillinase.

Specifications

Product Name

Penicillinase, CAS No.9001-74-5

Sinonimi

β-lactamase type I | β-Lactamase I

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, Sterili, EnzymoPure™

Specifiche e purezza

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, sterile, ≥10MU/EA; expressed in E.coli

Meccanismi biochimici e fisiologici

This protein is a beta-lactamase with a substrate specificity for penicillins. The class A beta-lactamase family has a specific amino-acid numbering system, sometimes called Ambler or ABL numbering and often misspelt as Amber. A multiple sequence alignmen

Bioattività

≥10MU/EA

Numero d'ordine

P00809

CAS

9001-74-5

Numero della Commissione Enzimi

EC 3.5.2.6

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

≥10MU/EA; expressed in E.coli

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

Definizione dell'unità

One activity unit is defined as the amount of enzyme required to hydrolyze 1 unit of penicillin G in 1 hour under conditions of pH 7.0 and 37˚C.

Riferimenti

1. Wenyao Chen, Shan Shan, Juan Peng, Daofeng Liu, Jun Xia, Bing Shao, Weihua Lai.  (2020)  Sensitive and hook effect–free lateral flow assay integrated with cascade signal transduction system.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2020.128465]

2. Shan Shan, Daofeng Liu, Qi Guo, Songsong Wu, Rui Chen, Kai Luo, Liming Hu, Yonghua Xiong, Weihua Lai.  (2016)  Sensitive detection of Escherichia coli O157:H7 based on cascade signal amplification in ELISA.  JOURNAL OF DAIRY SCIENCE,      [PMID:27394946] [10.3168/jds.2016-11320]

3. Chen Chen, Jun Lv, Wanyi Chen, Yang Xiang, Juan Zhang.  (2016)  Colorimetric β-lactamase inhibitor assay with double catalyzed signal amplification.  NEW JOURNAL OF CHEMISTRY,  40  (3): (1982-1987).  [PMID:] [10.1039/C5NJ02356D]

4. Lihong Li, Zhao Li, Wen Shi, Xiaohua Li, Huimin Ma.  (2014)  Sensitive and Selective Near-Infrared Fluorescent Off–On Probe and Its Application to Imaging Different Levels of β-Lactamase in Staphylococcus aureus.  ANALYTICAL CHEMISTRY,      [PMID:24844761] [10.1021/ac501288e]

5. Junfeng Liu, Liying Zhang, Haodi Ma, Haoyang Sun, Shu-ai Ge, Jieyi Liu, Shengdi Fan, Chunshan Quan.  (2024)  Quaternary ammonium chitosan-functionalized mesoporous silica nanoparticles: A promising targeted drug delivery system for the treatment of intracellular MRSA infection.  CARBOHYDRATE POLYMERS,      [PMID:39843087] [10.1016/j.carbpol.2024.123184]