pLenti-RNF121-sgRNA

Cat. No.: P749999

Specifications

Product Name

pLenti-RNF121-sgRNA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Stored at -20 ℃, valid for at least 6 months;Stored at -80 ℃, valid for at least one year.

Application Protocols

• Tested applications, recommended conditions, and positive controls: Not specified for this item; refer to CoA/Spec Sheet.
• General, non-operational guidance: Application details for CRISPR-based tools depend on delivery format, cell model, and nuclease system. Follow your institution’s validated SOPs and biosafety requirements. No stepwise protocols are provided here.

Biological Roles

• Target gene: RNF121 (RING finger protein 121), generally annotated as an E3 ubiquitin ligase candidate localized to endoplasmic reticulum membranes (literature). It is implicated in protein quality control and may intersect with ER-associated degradation (ERAD) pathways.
• Functional context (literature-general):
  • RING-type E3 ligases facilitate substrate ubiquitination by recruiting E2~Ub conjugates, influencing protein turnover and signaling.
  • RNF121 has been reported in studies exploring NF-κB pathway regulation and ER stress responses, though the breadth of validated substrates and interactors continues to evolve.
• Research utility of RNF121-directed sgRNAs:
  • Enable perturbation of RNF121 function for pathway mapping, identification of synthetic interactions, and assessment of downstream transcriptional or proteostatic effects.
  • Provide a route to examine redundancy/compensation among ER-localized RING E3s.
• Note: The above reflects general literature context. This product’s exact spacer sequence, PAM context, and species target (human/mouse, etc.) are not specified for this item; consult the CoA/Spec Sheet for item-specific targeting information.

Buffer Applications

• Not typically applicable. This item is a genetic tool rather than a buffer component.
• If buffer details are required for handling or dilution, they should be taken from the item’s CoA/Spec Sheet. As general practice, nuclease-free aqueous buffers are used for nucleic acid constructs; item-specific composition is not provided here.

Green Alternatives

• This product is a nucleic acid–based research tool; conventional solvent/toxicity tradeoffs discussed for organic reagents are not directly applicable.
• General sustainability considerations (literature-level):
  • Prefer aqueous, nuclease-free buffers and minimize use of hazardous organic solvents in any ancillary steps (e.g., DNA precipitation or cleanup).
  • Reduce single-use plastics where biosafety allows; employ validated decontamination methods that minimize environmental impact while maintaining biosafety compliance.
  • Consolidate cold shipments and aliquot to reduce repeat shipments and waste.
• Tradeoffs: Cold-chain requirements (-80°C storage; dry ice shipping) increase energy and shipping footprint; longer-term consolidated storage and careful aliquoting can mitigate repeated shipments while preserving material integrity.

Pharmaceutical Uses

• Research-use only reagent. Not for use in diagnostic procedures, clinical applications, or as a therapeutic product or excipient.
• No pharmacopeial status or formulation roles are specified for this item; refer to CoA/Spec Sheet if any compliance statements are provided.

Physical Properties

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer composition, and additives: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight/formula: Not applicable in a small-molecule sense; plasmid length (bp) or viral titer, if relevant, are not specified for this item; refer to CoA/Spec Sheet.
• Melting/boiling point, density, refractive index, logP, pKa: Not applicable to nucleic acid constructs; not specified for this item; refer to CoA/Spec Sheet.
• Solubility: DNA/RNA constructs are typically handled in aqueous biological buffers (literature); specific solvent system for this item is not specified; refer to CoA/Spec Sheet.
• Optical properties: Any A260/A280 or UV profile suitable for nucleic acids would be literature-general; item-specific values are not specified; refer to CoA/Spec Sheet.
• Note: As a genetic tool, conventional physicochemical specifications used for small-molecule reagents do not apply. Rely on the accompanying CoA/Spec Sheet for format (e.g., plasmid DNA in TE, or lentiviral prep), concentration, and any stabilizers (if present).

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• Research Use Only: This product is indicated for research use only. Not for human or animal therapeutic, diagnostic, or clinical applications.
• Typical quality attributes for lentiviral/plasmid sgRNA tools (literature-level, general):
  • Sequence identity and integrity of the sgRNA spacer and scaffold verified by sequencing.
  • For plasmid format: bacterial host selection marker performance and plasmid restriction map confirmation.
  • For viral format: titer/functional titer, sterility, and mycoplasma testing parameters may be reported. Item-specific metrics are not provided here; refer to CoA/Spec Sheet.
• Stabilizers/additives: Not specified for this item; refer to CoA/Spec Sheet.
• Documentation: For exact backbone, insert orientation, promoters, selection markers, and any accessory elements, consult the item’s CoA/Spec Sheet and sequence map, if provided.

Reaction and Applications

• This reagent is a gene-targeting tool rather than a chemical reactant. It is intended to provide an sgRNA sequence directed at the RNF121 locus, enabling CRISPR-based genome editing workflows when paired with a compatible Cas nuclease (high-level description; not a protocol).
• Typical research applications (non-operational, literature-level):
  • Gene knockout or transcriptional modulation of RNF121 using CRISPR-Cas systems (e.g., CRISPRko, CRISPRi, CRISPRa with appropriate effectors).
  • Mechanistic studies on RNF121 function in protein ubiquitination pathways and endoplasmic reticulum–associated processes.
  • Construction of isogenic cell models to examine RNF121-dependent signaling.
• Practical notes (conceptual, not stepwise): Choice of delivery format (plasmid vs. lentiviral) impacts cell-type accessibility and downstream selection strategies. Sequence verification of the sgRNA spacer and assessment of on-/off-target potential are standard pre-use checks (literature, general). All operational details should follow institutional SOPs and are not specified for this item.

Reaction Conditions

• Not applicable as a chemical reaction reagent. No temperatures, catalysts, or stoichiometries pertain to this product in a synthetic-chemistry sense.
• High-level, non-operational note: In gene-editing research, effective use of sgRNA constructs depends on compatible nuclease expression, cell-type delivery strategy, and suitable controls. Specific operational conditions are not provided for this item; consult institutional SOPs and the CoA/Spec Sheet.

Safety and Handling

• GHS classification, Signal Word, H/P statements, pictograms: Not specified for this item; refer to SDS.
• Storage conditions (item-specific, provided): Store at -80°C. Shipped on dry ice packs + cold packs to maintain cold chain integrity.
• General biosafety considerations (non-operational):
  • Lentiviral vectors and CRISPR tools should be handled under institutional biosafety guidelines (commonly BSL-2 practices for many vector formats); consult your IBC and the SDS for this specific item.
  • Prevent nuclease contamination; use clean, nuclease-free tools and PPE (lab coat, gloves, eye protection). Avoid generating aerosols.
• Incompatibilities and stability: Avoid repeated freeze–thaw cycles and prolonged exposure to ambient temperatures, which can degrade nucleic acids and reduce functional performance (literature, general).
• First aid/general response (overview; defer to SDS): In case of exposure to buffers or accidental contact, rinse affected area with water and seek appropriate medical evaluation per institutional policy. For spills, follow local biosafety procedures and decontamination methods recommended by your institution.
• Waste: Dispose of nucleic acid materials and consumables in accordance with institutional biosafety and local regulations.

Solvent Selection

• Not a small-molecule reagent; conventional solvent selection is not typically applicable.
• Handling context (general): Nucleic acid constructs are usually supplied or maintained in aqueous, nuclease-free buffers (e.g., TE or similar) per supplier documentation. Item-specific buffer composition is not specified for this item; refer to CoA/Spec Sheet.
• If downstream workflows require dilution or aliquoting, use nuclease-free water or appropriate biological buffers validated by your laboratory and institutional guidelines (general, literature).

Storage and Reconstitution

• Storage (item-specific): Store at -80°C.
• Shipping: Shipped on dry ice packs + cold packs to preserve stability during transit.
• Upon receipt (general, non-operational): Maintain the cold chain. Minimize freeze–thaw by preparing single-use aliquots in nuclease-free tubes under clean conditions. Exact buffer composition and concentration are not specified for this item; refer to CoA/Spec Sheet.
• Reconstitution: If supplied lyophilized or concentrated, follow the CoA/Spec Sheet for diluent and target concentration. For all handling, use nuclease-free materials. Item-specific reconstitution instructions are not provided here.
• Stability: Working stability and shelf life are not specified for this item; refer to CoA/Spec Sheet and SDS. Always verify integrity prior to critical experiments (e.g., by sequencing or titer assessment as appropriate to format, general).

Structure and Identity

• Item type: lentiviral CRISPR guide RNA construct targeting RNF121 (gene-level tool), not a small-molecule chemical.
• Name: pLenti-RNF121-sgRNA (indicative of a lentiviral vector backbone bearing an sgRNA cassette directed at the RNF121 locus).
• CAS, InChIKey, SMILES: Not applicable to plasmid/viral constructs; not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula / MW: Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general, literature-level description for typical pLenti sgRNA tools):
  • A lentiviral backbone with an RNA polymerase III promoter (e.g., U6) driving an sgRNA expression cassette containing a 20 nt spacer sequence complementary to the RNF121 genomic target and a conserved gRNA scaffold.
  • Long terminal repeats (LTRs) suitable for lentiviral packaging, plus common backbone elements (e.g., antibiotic resistance marker and origin of replication) for bacterial propagation. Specific backbone map is not provided here.
• 2D “structure” description (conceptual): circular double-stranded DNA plasmid (if supplied as plasmid) or an integrated lentiviral expression cassette (if used via lentiviral delivery) encoding a single guide RNA; no protein-coding information is implied by the product name. Exact construct organization and any selection markers are not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

• Not applicable in the context of small-molecule or polymer synthesis. This reagent is a genetic construct used for gene-targeting studies.
• For retrosynthetic or functional group transformation purposes in organic chemistry, please refer instead to small-molecule building blocks or catalysts. The present item serves as a biological tool for modulating RNF121 at the genomic level in research settings.

Target Specificity

• Intended target: RNF121 gene (general from product name).
• Species, spacer sequence, PAM context, and any validated off-target assessment: Not specified for this item; refer to CoA/Spec Sheet.
• Format (plasmid vs. packaged lentiviral particles), selectable markers, and promoter details: Not specified for this item; refer to CoA/Spec Sheet.
• Note: sgRNA specificity is determined by the 5′ spacer sequence and PAM requirements of the chosen Cas nuclease. Confirm sequence identity and species compatibility from item documentation prior to use (general guidance).

Domande frequenti

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.