Profilin 1 Mouse mAb - Anticorpo primario, specific to Profilin 1, Mouse IgG2b

Cat. No.: Ab191887

QUALITÀ & PUREZZA Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG2b

Clone Number

AB01/2E11

Conjugation

Unconjugated

Panoramica

Mouse anti Human Profilin 1 Antibody, clone AB01/2E11, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifiche

Product Name

Profilin 1 Mouse mAb - Anticorpo primario, specific to Profilin 1, Mouse IgG2b

Sinonimi

Actin binding protein antibody | ALS18 antibody | Epididymis tissue protein Li 184a antibody | OTTHUMP00000125244 antibody | PFN 1 antibody | Pfn antibody | PFN1 antibody | PROF1_HUMAN antibody | Profilin I antibody | Profilin-1 antibody | ProfilinI antib

Specifiche e purezza

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Meccanismi biochimici e fisiologici

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androg

Specie ospite

Mouse

Specificità

Profilin 1

Immunogeno

Recombinant protein corresponding to Human Profilin 1

Controllo positivo

WB: HeLa, HepG2, HT-29, K562, HEK293 and Jurkat whole cell lysates Flow: HeLa cells IF/ICC: U-2 OS cells

Coniugazione

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotipo

Mouse IgG2b

Tipo di catena leggera

lambda

SDS-PAGE

150.0 KDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Immagini

Western Blot: Profilin 1 Mouse mAb (Ab191887)

Profilin 1 Mouse mAb (Ab191887) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab191887) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 23.4 s

Western Blot: Profilin 1 Mouse mAb (Ab191887)

Profilin 1 Mouse mAb (Ab191887) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab191887) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 23.4 s

Flow Cytometry: Profilin 1 Mouse mAb (Ab191887)

Profilin 1 Mouse mAb (Ab191887) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Profilin 1 Mouse mAb (Ab191887) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).

ICC/IF: Profilin 1 Mouse mAb (Ab191887)

Profilin 1 Mouse mAb (Ab191887) - ICC/IF
IF analysis of Profilin 1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Profilin 1 Mouse mAb (Ab191887) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Applicazione

Applicazione

Dilution info

IF/ICC

1/200

WB

1/1,000 - 1/100,000; Predicted molecular weight: 15.0 KDa

Flow Cytometry

1/200 - 1/25000

Obiettivi associati (umani)

PFN1 Tbio Profilin-1 (0 Activities)

Discover Target: PFN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Profilin 1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.