The PI Staining Solution is suitable for the exclusion of dead cells from flow cytometric analysis. Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis.
The fluorescence emission maximum for DNA-bound PI is about 615 - 620 nm. When excited by a 488 nm laser, PI can therefore be detected in the B2 channel commonly used for PE or in the B3 channel commonly used for PerCP-Vio® 700, PE-Cy™5, etc.
Attention:
Propidium Iodide is a potential carcinogen. Avoid contact with skin and eyes. Wear suitable protective clothing, gloves, and eye/face protection.
Instructions for Use:
The suggested use of this solution for viability staining is 10 µl per million cells in 0.1 ml/test, and incubate for 10 minutes at 4 °C before analysis.