GRADE & PURITYSterile-filtered?Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.for Microscopy?Microscopy grade — reagents/stains suited to sample prep and imaging. Use in microscopy where clarity and low background are needed.Suitable for Immunofluorescence(IF)?IF grade — validated for immunofluorescence with low background staining. Use to localize targets in cells/tissue by fluorescence microscopy.1.0 mg/mL
sterile-filtered, Suitable for Immunofluorescence(IF), BioReagent, for microscopy, 1.0 mg/mL BioReagent,Filtrato asetticamente,per la microscopia,Suitable for Immunofluorescence(IF) for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
The PI Staining Solution is suitable for the exclusion of dead cells from flow cytometric analysis. Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis.
The fluorescence emission maximum for DNA-bound PI is about 615 - 620 nm. When excited by a 488 nm laser, PI can therefore be detected in the B2 channel commonly used for PE or in the B3 channel commonly used for PerCP-Vio® 700, PE-Cy™5, etc.
Attention:
Propidium Iodide is a potential carcinogen. Avoid contact with skin and eyes. Wear suitable protective clothing, gloves, and eye/face protection.
Instructions for Use：
It is recommended to titrate the PI Staining Solution to obtain optimal results as applications vary. The suggested use of this solution for viability staining is 2.5μg per million cells in 0.1 ml/test, and incubate for 10 minutes at 4 °C before analysis.
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