Protease from Streptomyces sp., CAS No.9036-06-0

CAS: 9036-06-0 Cat. No.: P755282 Numero EC: 232-909-5

GRADE & PURITY Type XXI,≥15 units/mg solid

Panoramica

Pronase E can be used to degrade antheraea pernyi silk fibroin films.

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Completely inactivated by heating above 80 °C for 15-20 minutes.

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.

This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein. Proteases from Streptomyces griseus and Streptomyces omiyaensis have been used in a study to identify residue 71 as a crucial residue for differences in topological specificities. Proteases have also been used in a study to investigate production of protease as a by-product of streptomycin production.

Specifications

Product Name

Protease from Streptomyces sp., CAS No.9036-06-0

Specifiche e purezza

Type XXI,≥15 units/mg solid

Meccanismi biochimici e fisiologici

Alkaline protease that is approximately twice as active at pH 11.0 than at the usual assay conditions for protease, pH 7.5 and 37 °C. By comparison, P5147 Type XIV protease is only approximately 25% as active at pH 11.0, 30 °C.

CAS

9036-06-0

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

Type XXI,≥15 units/mg solid

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Riferimenti

1. Liyun Zheng, Zhongwei Zhao, Li Chen, Wenjing Yang, Giorgio Ercolani, Pan Qin, Bin Lin, Mengzhu Han, Qiaoyou Weng, Yeyu Zhang, Bin Wu, Minjiang Chen, Zouying Yao, Shiji Fang, Jiansong Ji.  (2025)  Astragalin Induces Immunogenic Cell Death in Liver Cancer by Targeting NQO2 to Promote ROS-Mediated Endoplasmic Reticulum Stress Pathway.  FREE RADICAL BIOLOGY AND MEDICINE,      [PMID:40749898] [10.1016/j.freeradbiomed.2025.07.047]

2. Zheng Zhang, Wenjia Lu, Pengfei Liu, Mengjie Li, Xinyi Ge, Bin Yu, Zhengzong Wu, Guimei Liu, Nannan Ding, Bo Cui, Xiao Chen.  (2023)  Microbial modifications with Lycium barbarum L. oligosaccharides decrease hepatic fibrosis and mitochondrial abnormalities in mice.  PHYTOMEDICINE,      [PMID:37690228] [10.1016/j.phymed.2023.155068]

3. Jiajun Pan, Zhaopeng Xia, Haibao Zhang, Yang Lu, Yong Liu.  (2024)  One-step bleaching method for animal fiber by persulphate/hydrogen peroxide: Degradation mechanism of eumelanin.  Journal of Cleaner Production,      [PMID:] [10.1016/j.jclepro.2024.142256]

4. Xiang Ming, Lin Xiangdong, Chen Haiying.  (2025)  Luteolin Inhibits Ferroptosis of HUVEC by Regulating the Sirt1/Nrf2 Pathway.  CELL BIOCHEMISTRY AND BIOPHYSICS,      [PMID:40721691] [10.1007/s12013-025-01831-1]

5. Jun-Han Wang, Min Li, Peng-Fei Xie, Jia-Yao Si, Zhen-Jie Feng, Chuan-Feng Tang, Jian-Mei Li.  (2025)  Procyanidin C1 ameliorates aging-related skin fibrosis through targeting EGFR to inhibit TGFβ/SMAD pathway.  PHYTOMEDICINE,      [PMID:40315640] [10.1016/j.phymed.2025.156787]

6. Min Li, Jia-Yao Si, Peng-Fei Xie, Zhen-Jie Feng, Sheng-Jie Li, Feng Zhou, Jian-Mei Li.  (2025)  Procyanidin C1 from hawthorn modulates metabolic-inflammatory axis to combat vascular senescence in atherosclerosis.  FOOD RESEARCH INTERNATIONAL,      [PMID:41652755] [10.1016/j.foodres.2025.118224]

7. Yongjuan Wang, Xiaoli Xie, Jiajun Tian, Xiaoyu Jiang, Huiqing Jiang.  (2026)  Goosecoid promotes hepatic stellate cell epithelial-mesenchymal transition via transcriptional activation of serum- and glucocorticoid-induced protein kinase 1 in liver fibrosis.  BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,  1872  (4): (168169).  [PMID:41581345] [10.1016/j.bbadis.2026.168169]

8. Baoyu Wei, Hailong Shen, Chengtao Sun, Guangzhao Pan, Qianqian Xu, Xiaoqing Guan, Jiangjiang Qin, Guoyin Kai.  (2026)  Tanshinone I as a novel ferritinophagy inducer triggers ferroptosis to suppress gastric cancer growth.  PHYTOMEDICINE,      [PMID:42542057] [10.1016/j.phymed.2026.158645]