PSP , CAS No.3030735-86-2

CAS: 3030735-86-2 Cat. No.: P1449845 Formula: C₃₀H₂₂FN₃O₄ Peso molecolare: 507.51

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

PSP is a two-photon fluorescent probe for selective and sensitive detection of H 2 S n in live cells and tumor spheroids (Ex/Em = 440/640 nm). PSP can be utilized in ferroptosis research.

Specifiche

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nomi e identificatori

Peso molecolare

507.51

Application Protocols

No tested or validated application protocols are provided for PSP in the product data.

• Validated applications (WB, IHC, IF, FC, ELISA, enzymatic assays, etc.): Not specified for this item; refer to CoA/Spec Sheet.
• Recommended working concentrations/dilutions: Not specified for this item; refer to CoA/Spec Sheet.
• Positive controls/standards: Not specified for this item; refer to CoA/Spec Sheet.

Please consult the product’s datasheet or contact Aladdin Scientific technical support with the SKU and lot number for any available protocol guidance.

Biological Roles

Item-specific biological roles, targets, or pathways for PSP are not provided in the current product data. Therefore, no claims can be made about mechanisms, pathways, or functions.

General considerations for life-science reagents labeled “PSP” (contextual, not item-specific):

• The abbreviation “PSP” appears in literature across multiple domains (e.g., peptide sequences, polysaccharide preparations, small-molecule probes). Biological roles—if any—are entirely dependent on the precise identity and source, which are not specified here.
• If the product is a biomolecule (peptide/polysaccharide/protein fragment), typical investigations may include binding interactions, enzymatic processing, or structural biophysics (CD, DSC, ITC), all requiring confirmed sequence/structure.
• For small molecules, biological characterization often entails target-binding assays, cell-based phenotyping, and ADME profiling—none of which can be inferred without structure.

Recommendations:

• Obtain the CoA/Spec Sheet to confirm composition and purity.
• Before biological testing, verify identity and integrity by appropriate analytical methods (LC–MS, HRMS, NMR, SDS-PAGE/Western for proteins, carbohydrate analysis for polysaccharides).
• Establish baseline stability in the intended assay buffer (pH, ionic strength, temperature) and assess adsorption to plastics or filters when working at low concentrations.

Note: This product is for research use only. No medical, diagnostic, or therapeutic claims are made or implied.

Buffer Applications

Specific buffer systems validated for PSP are not provided. The following guidance outlines a general approach to buffer selection and preparation for life-science reagents when identity and pKa are not yet known.

General strategy:

• Begin with neutral buffers: PBS (10–50 mM phosphate, 150 mM NaCl, pH 7.2–7.4), HEPES (10–50 mM, pH 7.0–7.5), or Tris (10–50 mM, pH 7.2–7.8). Evaluate solubility and stability over 24–72 h at 2–8°C and room temperature.
• If poor solubility: adjust pH by ±0.5–1.0 units; introduce co-solvent (5–20% v/v EtOH or MeOH) if compatible with downstream assays; or add salt (50–150 mM NaCl) to reduce aggregation for peptide/biopolymer candidates.
• For LC–MS–compatible workflows: use volatile buffers (e.g., 10–50 mM ammonium formate/acetate, pH 3.5–6.5) and avoid non-volatile salts/surfactants.

Practical tips:

• Filter buffers (0.22 μm) and degas when air sensitivity or foaming affects performance.
• Document precipitation/clarity upon dilution, and consider slow addition of buffer to concentrated stock to avoid salting-out.
• Avoid strong chelators (EDTA) or reducing agents (DTT/TCEP) unless clearly needed, as they can interfere with metalloproteins or redox-sensitive analytes.

Quality controls:

• Track pH drift over time; record conductivity and osmolality if relevant to cells or biophysics.
• Establish hold-time studies (e.g., 1 day, 1 week at 2–8°C) to ensure stability of PSP in the chosen buffer before critical experiments.

Green Alternatives

Without a defined structure or declared application for PSP, greener choices focus on solvent selection, energy input, and waste minimization rather than reagent substitution.

Greener solvent hierarchy (general):

• Prefer water and buffered aqueous media when feasible.
• Use bio-based alcohols (EtOH, i-PrOH) over higher-toxicity polar aprotics when solubility allows.
• Minimize DMSO/DMF/NMP; if required, keep volumes low and implement closed handling with dedicated waste streams.

Comparison snapshots (general literature guidance):

| Context | Conventional | Greener option | Trade-offs | |---|---|---|---| | Stock solutions for assays | DMSO | EtOH, glycerol, aqueous co-solvent | Lower solubility for very nonpolar analytes | | Peptide handling | HFIP/TFA-rich systems | Aqueous buffers, volatile salts (NH4HCO3) | May need optimization to avoid aggregation | | Chromatography | ACN-rich gradients | MeOH-rich gradients, water-rich methods | Viscosity/backpressure, selectivity shifts |

Operational improvements:

• Right-sizing: Prepare only the quantity needed; aliquot to reduce waste from freeze–thaw cycles.
• Energy: Favor ambient temperature protocols; avoid unnecessary long heating/stirring.
• Analytical first: Use microscale solubility and stability screens before scaling up.

Documentation and disposal:

• Maintain segregated waste for halogenated and high-boiling solvents. Explore solvent recycling where validated.

All green recommendations should be reconciled with performance requirements and the verified identity/compatibility of PSP.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or formulation use is specified for PSP in this listing. The product is designated for research use only and is not intended for human or veterinary applications.

General, non-clinical formulation context (not item-specific):

• If PSP is later confirmed to be a small molecule, early preformulation studies might include solubility profiling (pH 2–9), intrinsic dissolution, and compatibility with common excipients (lactose, MCC, mannitol) solely for laboratory feasibility assessments.
• If PSP is a peptide/biopolymer, exploratory formulation might assess buffer systems (histidine, citrate, phosphate), tonicity agents (NaCl, sucrose), and stabilizers (polysorbates, amino acids) to understand basic stability drivers (aggregation, oxidation, deamidation). These activities are strictly preclinical research.

Good practices for research formulation work:

• Use analytical characterization (HPLC/UPLC, LC–MS, DSC, CD, SEC) to monitor stability under stress (freeze–thaw, agitation, light).
• Employ small-scale DoE screens to map pH, ionic strength, and excipient effects prior to any scale-up.

Compliance note:

• Do not incorporate PSP into products for human or veterinary use. For any regulated development, obtain comprehensive specifications, residual impurity profiles, and stability data for the specific lot, and follow relevant regulatory guidance.

Physical Properties

Item-specific data:

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting/boiling point, density, refractive index, pKa, logP, UV cutoff, water content, residual solvents, metal content: Not specified for this item; refer to CoA/Spec Sheet.

General considerations (literature/practice-based, not item-specific):

• If PSP is a biopolymer/peptide, it will likely be a non-volatile solid with variable hygroscopicity; solubility often improves in aqueous buffers (pH 6–8) or with salts (e.g., 50–150 mM NaCl) and mild warming (≤37°C).
• If PSP is a small organic molecule, initial solubility screening commonly includes water, PBS, MeOH, EtOH, acetonitrile, DMSO, and DMF. Begin with low concentrations (1–5 mg/mL) and increase as needed.
• UV/Vis: For peptides and many organics, a scan from 200–400 nm is informative; nucleophilic residues and aromatic groups (Trp/Tyr/Phe, phenyl/heteroaryl) often show characteristic absorbance bands. Establish an extinction coefficient only after structure is known.
• Hygroscopicity: Unknown. Store closed under dry conditions. Weigh quickly in a low-humidity environment if precise massing is required.
• Thermal behavior: Unknown. Avoid prolonged exposure to elevated temperatures. Follow the provided storage at −20°C.

Practical tips:

• Record a solubility log upon first use, noting solvent, pH, ionic strength, and any need for sonication/filtration. Validate clarity by nephelometry or simple visual inspection.
• Filter aqueous solutions through 0.22 μm if sterility or particulate control is required.

Quality and Grades

Item-specific grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret grades (general guidance for life-science reagents):

• Research Grade: Suitable for exploratory R&D; may include standard identity/assay, moisture, and impurity profiles. UV/Vis background and endotoxin levels may not be controlled unless stated.
• Biochemical/Cell Culture Grade: Typically features tighter controls on bioburden, endotoxin, and trace contaminants. Batch-to-batch functional testing may be provided.
• HPLC/LCMS Grade (for solvents/standards): Low UV absorbance and particulates; often filtered and stabilized. Not applicable unless PSP is supplied as/with a solvent.

CoA/Spec expectations for PSP (what to look for):

• Identity tests: NMR/HRMS/LC-MS, HPLC/UPLC purity or peptide mapping (if applicable).
• Assay/purity: % area by HPLC, or amino acid analysis/sequence verification for peptides.
• Residuals: Water content (KF), residual solvents, inorganic ash, metals (ICP-MS) as applicable.
• Bioburden/endotoxin: If intended for sensitive biological systems.

Batch management and traceability:

• Record lot number and retain a copy of the CoA with your experimental records. For critical applications, perform an incoming QC check (appearance, pH of solution, HPLC profile) to confirm suitability.

Note: This product is marked “For research use only.” It is not intended for human or veterinary applications.

Reaction and Applications

No specific synthetic or biochemical application claims are provided for PSP in the product data. Accordingly, the following guidance is general and should be adapted only after confirming the identity and compatibility from the CoA/Spec Sheet.

Potential laboratory contexts (general):

• Biochemical assays: If PSP is a biomolecule, it may be used as an analyte, ligand, or standard in ELISA-like or binding assays once sequence/structure is confirmed.
• Analytical chemistry: Prepare calibration stocks in validated solvents for HPLC/UPLC or LC–MS. Establish response linearity, stability, and carryover.
• Materials/interaction studies: Assess solubility, aggregation, or binding to matrices (e.g., resins, membranes) using buffer screens.

If PSP were to be used in synthetic contexts (only as general literature guidance):

• For small molecules: Explore derivatization under mild conditions (amide coupling, carbamate/urethane formation, click chemistry) only after understanding functional groups.
• For peptides/biopolymers: Conjugation strategies may include NHS-ester labeling of primary amines, maleimide–thiol coupling for Cys residues, or azide–alkyne click when prefunctionalized.

Good practice:

• Validate identity and purity by LC–MS or orthogonal methods before application.
• Run small-scale pilot reactions/assays to confirm behavior before committing precious sample.
• Record detailed conditions (pH, temperature, time, concentration) for reproducibility and troubleshooting.

Reaction Conditions

No item-specific reaction conditions are provided for PSP. If PSP is to be used in chemical modification or conjugation, the following are general literature-based conditions to consider only after confirming functionality.

General conjugation/derivatization conditions (examples, not specific to this item):

• Amide coupling (carboxyl present): EDC/HOBt or HATU, DIPEA, DMF or aqueous buffer (pH 6–7) for water-soluble substrates; 0–25°C, 0.5–4 h. Quench with water/buffer and purify by RP-HPLC.
• NHS-ester labeling (primary amine present): pH 7.5–8.5 in phosphate/borate buffer; 1–2 eq label, 15–60 min at RT; avoid competing amines (e.g., Tris) during reaction.
• Maleimide–thiol coupling (thiol present): pH 6.5–7.0 in phosphate buffer with 1–5 mM EDTA to limit metal-catalyzed oxidation; 10–60 min, RT; protect from light if chromophore present.
• Click chemistry (azide/alkyne present): CuAAC with CuSO4/sodium ascorbate and TBTA ligand in t-BuOH/H2O or DMSO/H2O, RT–40°C; strain-promoted SPAAC in purely aqueous media if copper-free needed.

Analytical controls:

• Monitor by LC–MS or HPLC; confirm mass shifts and purity. Assess residual copper (if used) for sensitive biological applications.

Caveats:

• Do not apply these conditions to PSP without verifying that the necessary functional groups exist and that the material is stable under the chosen conditions.

Safety and Handling

Hazard information (item-specific):

• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• GHS Classification: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.

General laboratory safety (good practice; defer to SDS for authoritative guidance):

• PPE: Lab coat, safety glasses or chemical splash goggles, and appropriate chemically resistant gloves. Use a certified chemical fume hood when handling powders, aerosols, or volatile solvents.
• Avoid: Inhalation of dust/aerosols, skin/eye contact, ingestion. Do not pipette by mouth. Wash hands thoroughly after handling.
• Storage incompatibilities: Unknown for this item. Until known, segregate from strong acids/bases, oxidizers/reducers, and moisture-sensitive materials. Store closed, dry, and protected from light as prudent.
• Spill response: For solids, gently cover and pick up using dampened disposable towels or HEPA-filtered vacuum; avoid dust generation. For solutions, contain with absorbent. Dispose per institutional and regulatory requirements.
• First aid (general): If inhaled—move to fresh air; if on skin—wash with soap and water; if in eyes—rinse with water for ≥15 minutes; if ingested—rinse mouth. Seek medical attention in all exposure scenarios and provide the SDS.
• Thermal/chemical stability: Unknown; avoid heating and mixing with reactive reagents until compatibility is established.

Always consult the product’s SDS and CoA for definitive safety classifications and handling instructions.

Solvent Selection

Because the structure of PSP is not provided, solvent selection should follow a systematic screening strategy tailored to the intended use (analytical measurement, biochemical assay, or formulation).

Recommended stepwise screen (general guidance):

• Aqueous first: Water, PBS (pH 7.2–7.4), and 10–50 mM HEPES or Tris buffers. Adjust pH incrementally (pH 6–8.5) if ionizable groups are suspected.
• Polar protic: MeOH, EtOH, i-PrOH for small molecules with H-bonding capability; often miscible with water for co-solvent systems (5–30% v/v) to improve solubility while maintaining biocompatibility.
• Polar aprotic: DMSO and DMF as last-resort stock solvents for recalcitrant organics; prepare concentrated stocks (e.g., 10–100 mM) and dilute into aqueous media keeping final DMSO ≤0.5–1% for cell-based assays.
• Nitriles/esters: Acetonitrile or ethyl acetate for chromatography or partitioning evaluations (non-biological contexts).

Practical tips:

• Start at low concentration (1–5 mg/mL). Use brief sonication and gentle warming (≤37°C). Avoid high pH/strong acids unless compatible.
• If precipitation occurs on dilution from DMSO to buffer, dilute more slowly, use surfactants (e.g., 0.01–0.1% polysorbate) only if compatible, or include a small fraction of co-solvent.
• For peptide/biopolymer candidates, include salted buffers (e.g., 150 mM NaCl) to mitigate aggregation and consider chaotropes (urea/guanidine) only for denaturation workflows.

Documentation:

• Record solvent, pH, ionic strength, temperature, and time-to-clarity. Retain this record with your method to ensure reproducibility.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Store at −20°C (as provided in Product Data).
• Shipped In: Ice chest + ice pads (as provided in Product Data).

General guidance (not item-specific; adapt after reviewing the CoA):

• Protection: Store tightly closed in a desiccated environment and protect from light until identity and light-sensitivity are known.
• Aliquoting: Upon first opening, divide into small, single-use aliquots to avoid repeated freeze–thaw cycles.
• Reconstitution solvents: Start with sterile water or buffer (PBS/HEPES/Tris, pH ~7.2–7.5). If insoluble, consider minimal volumes of DMSO or EtOH to prepare a concentrated stock, then dilute into buffer slowly to prevent precipitation. Document final solvent composition.
• Concentration targets: Begin at 1–5 mg/mL (or 1–10 mM for small molecules) and adjust based on solubility and intended use.
• Technique: Use gentle vortexing, brief sonication, and mild warming (≤37°C). Avoid vigorous agitation if foaming or denaturation is a risk (biopolymers/peptides).
• Sterility/clarity: If solutions are for cell-related assays, filter through 0.22 μm after dissolution. Verify absence of particulates.
• Stability assessment: Prepare a small test aliquot and monitor by HPLC/UPLC or LC–MS over several days at 4°C and room temperature to establish working hold times.

Disposal: Follow institutional chemical/biological waste policies. Always refer to the product SDS for definitive handling and stability information.

Structure and Identity

Short description: PSP is listed as a life-science research reagent under SKU P1449845. Structural identifiers are not supplied in the current listing; consult the CoA/Specification Sheet for definitive details.

• SKU: P1449845
• Product Name (catalog): PSP
• CAS: 3030735-86-2 (item-specific)
• Category: Life Science (研究/实验用途)
• Research Use Note: For research use only

Item-specific identifiers (as provided):

• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

General structural notes (contextual, literature/general):

• “PSP” is an abbreviation used in diverse subfields (e.g., peptides, polysaccharide-containing preparations, small-molecule scaffolds). Without a supplied structure, functional groups, ring systems, or stereochemical features cannot be ascertained.
• If the material is a peptide/protein, expect amide backbones and side-chain functionalities; if polysaccharide-derived, expect polyhydroxylated carbohydrate motifs; if a small molecule, features are case-dependent.

Practical identity guidance:

• Use the CAS (3030735-86-2) and batch/lot number on the product label to request the CoA, which should list formula, exact mass, and structural identifiers.
• If you will use PSP in regulated or analytical workflows, confirm identity by orthogonal methods (e.g., HPLC/UPLC retention vs. standard, HRMS, NMR where applicable) after reviewing the CoA.

Synthetic Utility

The product data do not define PSP’s chemical structure, so specific synthetic transformations or retrosynthetic roles cannot be assigned.

General possibilities (literature-informed, not item-specific):

• If PSP is a small organic molecule: Potential utility could include use as a reference standard, a scaffold for SAR exploration, or a coupling partner in mild derivatizations (amide formation, esterification, click chemistry) once functional groups are known.
• If PSP is a peptide/biopolymer: Synthetic utility would be limited to bioconjugation or labeling (e.g., NHS ester reactions with Lys, maleimide–thiol coupling with Cys, oxime ligation with introduced aldehydes), contingent on sequence/composition.

Best practices before any synthetic use:

• Obtain the CoA and verify identity by LC–MS/HRMS/NMR (as applicable) and purity by HPLC/UPLC.
• Conduct small-scale compatibility checks to confirm stability to bases/acids, nucleophiles/electrophiles, heat, and light.
• Establish quench/workup conditions that preserve PSP integrity (neutral quench where possible; minimize exposure to strong acids/bases if peptide-like).

Documentation:

• Record equivalents, concentration, temperature, and time meticulously. Archive chromatograms/spectra for traceability and to support reproducibility.

Target Specificity

Target/epitope information, species reactivity, clone, and isotype are applicable to antibodies and affinity reagents. For PSP (SKU P1449845), no such data are provided.

• Antigen/Target: Not specified for this item; refer to CoA/Spec Sheet.
• Species Reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Clone/Isotype: Not applicable/not specified.

Note: If PSP is used in binding studies or as a ligand, target specificity must be determined experimentally after the structure is confirmed and appropriate assays are developed.